ISRel13
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004041 | ND | Rhizobium etli | Rhizobium etli CFN 42 symbiotic plasmid p42d Rhizobium etli Kim 5 |
DNA section
IS Length : 900 bp
Ends
IR Length : 12/16
IRL : GGCGGTGTGGACTCTTGGGATTCCCAAGGCTGAGCAAATCAGATTCAAGA
IRR : GGTCGTGTGGACAGTTATCTGATCCATAGCATTGTCGCCGCGATAGTGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTCAGGCCT | TA | GAGCTTTCGG | 2 |
| ATGCCAGCGC | TA | AGCATGGTGG | 2 |
DNA sequence
GGCGGTGTGGACTCTTGGGATTCCCAAGGCTGAGCAAATCAGATTCAAGACTGTCTTTTGGAGGCAGTCATGGGTGTTTTGGATCGTCTGATCCTTCGGG
ACGAGCAGTGGGAGCGGATATCGCACCACATCATTGGTGACGAGCGCACACGCGGTTCGTCCGGCCGCGACAACCGCATGTTTATGGAAGCGGTGCTTTG
GATTGTGCGAACGGGCTCGCCCTGGCGCGATCTGCCAGAAGTGTTCGGCGAGTGGAACAGTGTCTTCCGTCGTTTCAGCCGCTGGAGCCGGAAGGGCATC
TGGTGGCGCATATTCGAGGCGATGGCGGACGATCCAGACTTCGAGTACTTGATTGTTGATTCCACCATCATCCGCGCCCATCAGCACGCTGCCGGCGCAA
AAAAGGGGCTGAAGATCAAGCCCTTGGCCGCTCCCGCGGCGGATTGAGCACCAAGATCCACATGGCCGTGCGGGGTCTCGGTTGCCCGGTCCGCTTCACA
TTGACCGCAGGCCAGAAGGGCGATGCGCCGCAAGCCGACGCCCTGATCGAGGGCCTACCCGCCGACATCATCATGGCCGATACCGCCTATGACAGCAATC
ACCTGCGCAATGCCATCGCCGATAAGGGCGCCGTGGCAGTGATCCCCAACAATCCTTCGAGTGCGCGAAAATACCCGCTCGACAAACATCTCTATGCTCA
GCGACACCTGATCGAATGCTGCTTTTCTAAACTCAAGCAGTTCAGAAGGGTCGCGACGCGCTTCGAGAAAACCGCCGAAAACTACCTCGCCGTCGTCACT
ATCGCGGCGACAATGCTATGGATCAGATAACTGTCCACACGACC
ACGAGCAGTGGGAGCGGATATCGCACCACATCATTGGTGACGAGCGCACACGCGGTTCGTCCGGCCGCGACAACCGCATGTTTATGGAAGCGGTGCTTTG
GATTGTGCGAACGGGCTCGCCCTGGCGCGATCTGCCAGAAGTGTTCGGCGAGTGGAACAGTGTCTTCCGTCGTTTCAGCCGCTGGAGCCGGAAGGGCATC
TGGTGGCGCATATTCGAGGCGATGGCGGACGATCCAGACTTCGAGTACTTGATTGTTGATTCCACCATCATCCGCGCCCATCAGCACGCTGCCGGCGCAA
AAAAGGGGCTGAAGATCAAGCCCTTGGCCGCTCCCGCGGCGGATTGAGCACCAAGATCCACATGGCCGTGCGGGGTCTCGGTTGCCCGGTCCGCTTCACA
TTGACCGCAGGCCAGAAGGGCGATGCGCCGCAAGCCGACGCCCTGATCGAGGGCCTACCCGCCGACATCATCATGGCCGATACCGCCTATGACAGCAATC
ACCTGCGCAATGCCATCGCCGATAAGGGCGCCGTGGCAGTGATCCCCAACAATCCTTCGAGTGCGCGAAAATACCCGCTCGACAAACATCTCTATGCTCA
GCGACACCTGATCGAATGCTGCTTTTCTAAACTCAAGCAGTTCAGAAGGGTCGCGACGCGCTTCGAGAAAACCGCCGAAAACTACCTCGCCGTCGTCACT
ATCGCGGCGACAATGCTATGGATCAGATAACTGTCCACACGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 378 bp | 125 aa | 70 | 447 | + | No |
Description : First part of the transposase
ORF sequence :
MGVLDRLILRDEQWERISHHIIGDERTRGSSGRDNRMFMEAVLWIVRTGSPWRDLPEVFGEWNSVFRRFSRWSRKGIWWRIFEAMADDPDFEYLIVDSTI
IRAHQHAAGAKKGLKIKPLAAPAAD
IRAHQHAAGAKKGLKIKPLAAPAAD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 471 bp | 156 aa | 360 | 830 | + | No |
Description : Second part of the transposase
ORF sequence :
FHHHPRPSARCRRKKGAEDQALGRSRGGLSTKIHMAVRGLGCPVRFTLTAGQKGDAPQADALIEGLPADIIMADTAYDSNHLRNAIADKGAVAVIPNNPS
SARKYPLDKHLYAQRHLIECCFSKLKQFRRVATRFEKTAENYLAVVTIAATMLWIR
SARKYPLDKHLYAQRHLIECCFSKLKQFRRVATRFEKTAENYLAVVTIAATMLWIR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 761 bp | 253 aa | 70 | 830 | + | Yes |
Chemistry : DDE
ORF sequence :
MGVLDRLILRDEQWERISHHIIGDERTRGSSGRDNRMFMEAVLWIVRTGSPWRDLPEVFGEWNSVFRRFSRWSRKGIWWRIFEAMADDPDFEYLIVDSTI
IRAHQHAAGAKKGAEDQALGRSRGGLSTKIHMAVRGLGCPVRFTLTAGQKGDAPQADALIEGLPADIIMADTAYDSNHLRNAIADKGAVAVIPNNPSSAR
KYPLDKHLYAQRHLIECCFSKLKQFRRVATRFEKTAENYLAVVTIAATMLWIR
IRAHQHAAGAKKGAEDQALGRSRGGLSTKIHMAVRGLGCPVRFTLTAGQKGDAPQADALIEGLPADIIMADTAYDSNHLRNAIADKGAVAVIPNNPSSAR
KYPLDKHLYAQRHLIECCFSKLKQFRRVATRFEKTAENYLAVVTIAATMLWIR
Blast result :
Comments
ISRel13 is 70% aa similar to ISAli9.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References