ISRel6
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004041 | ND | Rhizobium etli | Rhizobium etli CFN 42 symbiotic plasmid p42d Rhizobium etli CFN 42 plasmid p42b Rhizobium etli CFN 42 |
DNA section
IS Length : 1047 bp
Ends
IR Length : 18/26
IRL : GAGCGGATCATTTCTTATCGGAATCGGTGCGGGATTCCCAAATCAGCAGA
IRR : GAGCCTTTCATGTTTTGACGGAAGCGTAGCCAGCATTTGAGAAGTAGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGACTTGCTC | TA | GGTCGCTCGT | 2 |
| AATGAAACGC | TA | GCGTAACGAT | 2 |
| CGCCAACTGC | TA | GGCATCGTCT | 2 |
| CGCATTGCGT | TA | GCCCCTCCCC | 2 |
| CTGGCTTCCT | TA | GGCGGCACGC | 2 |
DNA sequence
GAGCGGATCATTTCTTATCGGAATCGGTGCGGGATTCCCAAATCAGCAGATTTGTGATTCATGATGGATGCTGGAATGGAGGCCAGCATCCATGACGCGA
CCCTATTCGAATGATCTTCGCGAGCGAGTCATTGCAGCGGTTGTGGACGGTCAGAGCTGCCGGGTGGTGGCGGAGCGGTTCAACATAGCTATCTCATCCG
TGGTGAAGTGGTCGCAGCGTTATCGGGCCACCGGTAGTGTGTCGCCCGGTAAGATGGGCGGCCATCGCCGACGTGTGCTGGAGCCGCACCGCGCCTTCAT
TGTCGAGCAGATCGAGCAGACATCACATCTGACGCTACATCGGCTGAAGGATGAGTTAGCCGCCCGCGGTGTGAGCGTCTCCCACAATGCCATCTGGCAA
TTCATGCGCCGCGAGGGCTTGAGCTTTAAAAAAAACGCTGTTCGCCCTTGAGCAGGGCCGCGCCGACATTGCCCGCCGCAGAGCTCGCTGGAAAGCCTGG
CAAGACCGCTTCGATCCGAAGCGGCTCGTCTTTATTGACGAGACCTGGATCCGCACCAACATGGCGCCGTTACGCGGCTGGGGGCCGAGGGGCAAAAGAT
TACGCGGCTTTGCCCCGCATGGCCGCTGGCGCACCCTCACCTTCCTCGGCGCCCTACGCTGCGACAAGCTCACTGCACCCTGCGTCTTCGACGGTCCCAT
CAATGGCGAATGCTTTCACGCCTATGTCCGCCAGCAGTTGATCCCGACCCTCAAACCTGGCGACATCGTCATCCTCGATAATCTCGGCTCTCACAAAGCC
AAGGCCATCCGCGATGCCATCAGGGCCGTCGGCGCCAGGCTCTGGTTCCTGCCGAAATATTCCCCCGACCTTAACCCGATCGAGCAGGCCTTCGCCAAGA
TCAAGCACTGGATGCGCCAGGCCCAAAAGCGAACCATCGAGGAAACCTGGCGCTATCTGGGCATACTCGCCGACACTATCAAGCCAGAGGAATGCGCCAA
CTACTTCTCAAATGCTGGCTACGCTTCCGTCAAAACATGAAAGGCTC
CCCTATTCGAATGATCTTCGCGAGCGAGTCATTGCAGCGGTTGTGGACGGTCAGAGCTGCCGGGTGGTGGCGGAGCGGTTCAACATAGCTATCTCATCCG
TGGTGAAGTGGTCGCAGCGTTATCGGGCCACCGGTAGTGTGTCGCCCGGTAAGATGGGCGGCCATCGCCGACGTGTGCTGGAGCCGCACCGCGCCTTCAT
TGTCGAGCAGATCGAGCAGACATCACATCTGACGCTACATCGGCTGAAGGATGAGTTAGCCGCCCGCGGTGTGAGCGTCTCCCACAATGCCATCTGGCAA
TTCATGCGCCGCGAGGGCTTGAGCTTTAAAAAAAACGCTGTTCGCCCTTGAGCAGGGCCGCGCCGACATTGCCCGCCGCAGAGCTCGCTGGAAAGCCTGG
CAAGACCGCTTCGATCCGAAGCGGCTCGTCTTTATTGACGAGACCTGGATCCGCACCAACATGGCGCCGTTACGCGGCTGGGGGCCGAGGGGCAAAAGAT
TACGCGGCTTTGCCCCGCATGGCCGCTGGCGCACCCTCACCTTCCTCGGCGCCCTACGCTGCGACAAGCTCACTGCACCCTGCGTCTTCGACGGTCCCAT
CAATGGCGAATGCTTTCACGCCTATGTCCGCCAGCAGTTGATCCCGACCCTCAAACCTGGCGACATCGTCATCCTCGATAATCTCGGCTCTCACAAAGCC
AAGGCCATCCGCGATGCCATCAGGGCCGTCGGCGCCAGGCTCTGGTTCCTGCCGAAATATTCCCCCGACCTTAACCCGATCGAGCAGGCCTTCGCCAAGA
TCAAGCACTGGATGCGCCAGGCCCAAAAGCGAACCATCGAGGAAACCTGGCGCTATCTGGGCATACTCGCCGACACTATCAAGCCAGAGGAATGCGCCAA
CTACTTCTCAAATGCTGGCTACGCTTCCGTCAAAACATGAAAGGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 360 bp | 119 aa | 92 | 451 | + | No |
Description : First part of the transposase
ORF sequence :
MTRPYSNDLRERVIAAVVDGQSCRVVAERFNIAISSVVKWSQRYRATGSVSPGKMGGHRRRVLEPHRAFIVEQIEQTSHLTLHRLKDELAARGVSVSHNA
IWQFMRREGLSFKKNAVRP
IWQFMRREGLSFKKNAVRP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 618 bp | 205 aa | 423 | 1040 | + | No |
Description : Second part of the transposase
ORF sequence :
ALKKTLFALEQGRADIARRRARWKAWQDRFDPKRLVFIDETWIRTNMAPLRGWGPRGKRLRGFAPHGRWRTLTFLGALRCDKLTAPCVFDGPINGECFHA
YVRQQLIPTLKPGDIVILDNLGSHKAKAIRDAIRAVGARLWFLPKYSPDLNPIEQAFAKIKHWMRQAQKRTIEETWRYLGILADTIKPEECANYFSNAGY
ASVKT
YVRQQLIPTLKPGDIVILDNLGSHKAKAIRDAIRAVGARLWFLPKYSPDLNPIEQAFAKIKHWMRQAQKRTIEETWRYLGILADTIKPEECANYFSNAGY
ASVKT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 949 bp | 315 aa | 92 | 1040 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRPYSNDLRERVIAAVVDGQSCRVVAERFNIAISSVVKWSQRYRATGSVSPGKMGGHRRRVLEPHRAFIVEQIEQTSHLTLHRLKDELAARGVSVSHNA
IWQFMRREGLSFKKTLFALEQGRADIARRRARWKAWQDRFDPKRLVFIDETWIRTNMAPLRGWGPRGKRLRGFAPHGRWRTLTFLGALRCDKLTAPCVFD
GPINGECFHAYVRQQLIPTLKPGDIVILDNLGSHKAKAIRDAIRAVGARLWFLPKYSPDLNPIEQAFAKIKHWMRQAQKRTIEETWRYLGILADTIKPEE
CANYFSNAGYASVKT
IWQFMRREGLSFKKTLFALEQGRADIARRRARWKAWQDRFDPKRLVFIDETWIRTNMAPLRGWGPRGKRLRGFAPHGRWRTLTFLGALRCDKLTAPCVFD
GPINGECFHAYVRQQLIPTLKPGDIVILDNLGSHKAKAIRDAIRAVGARLWFLPKYSPDLNPIEQAFAKIKHWMRQAQKRTIEETWRYLGILADTIKPEE
CANYFSNAGYASVKT
Blast result :
Comments
ISRle6 is 71% aa similar to ISGdi6.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
References