ISSpu24
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAWY01000042 | ND | Shewanella putrefaciens | Shewanella putrefaciens 200 |
DNA section
IS Length : 2524 bp
Ends
IR Length : 20/22
IRL : GTAACCGTACGGCCACTACATCTTTTATTTAATTATCCTTAAGGCATCCT
IRR : GTAACCGTCCGGCCACTACACCCAGTATCTTCATTTAAGTGTCCACTTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGACCCAAT | GATCCTAA | GAATCATTTG | 8 |
DNA sequence
GTAACCGTACGGCCACTACATCTTTTATTTAATTATCCTTAAGGCATCCTATCCACTTTATCAGCCAGGATATGACCGATGTCAGATATTATTCATCACA
CTAGTCGCCGGATCTTCTCGCCTCAATATAAATTAGAGATCGTGAATGAGGCGTTAACCAAGCAGTTATCAACTGCCCAAATTGCACAAAAGCACCACAT
CAATAACAACCAAGTTTTTCACTGGTGTCGCGAATATAAACTTGGTGTGGCAAGATGGGTAAAAGTAGCAAGCAACAACCCTGTGATTGCGACACAAGAT
CCCCCTGGAGAGACCAAGTTTTTACCCATCACGACTCAATCCAGCTCCAATACTGTCATGCGCAATGATCAACAACAGACGCCAAACTCAAGTACGGTGG
TCGTTGAGCTAGCCAGTGGGCACGTGCTCAAGCTCACACATCCCACGGCTGAGACGATCAAACAGATCTTATTGGTTATCGTATGATCACTTTATCTCAT
GATGTACGGATCTGGTTATGTGCCGGCCACACCGATATGCGTAAAGGCTTTGATAGTTTATCTGCGATTGCGCAGCATATATTACAAAAAGATCCCTTTA
ATGGGCATGTTTTTGTGTTTCGCGGCAAACGTGGTGATCGGGTTAAATTACTATGGTGGGATGGCCAAGGATTATGTCTGTATTATAAACGTTTGGAGCA
CGGACGTTTTGTGTGGCCGAGCGCAACATCCGGTGCTGTTCACTTATCACAAGCCCAATTATCCATGCTATTAGAGGGCATTGATTGGCGCCCACCGGCA
AGGCGATATCGACCGGGAAATAGTGCGTAAAAAACCGTGAATTTATTGCTTTATCCCGTCTTTTTATTGGGCTTGAGTGGTATAATTAAGCTCATGAAAA
CAGGCCGTCAAGCACTTCCAACCGATACTCATGCATTGCAACAAATGGTGTTGCAGATGCAATCTGAGCTGTCTGAAAATAATAAGAAACTGTCCGCTCA
AGACTCCGTTATCGATAACCTACGCCATCAATTAGCGGTATTAAAGCGTGCGCGTTTTGGTCGATCATCAGAGCAAGTTGAGCAAAATATCCATCAGTTG
GAATTACAGCTTGAAGAGCTAGAAATCGCCCAAGCACAGACGGCCCCTGGTAGCCGTGCTGCAGGCACGGTCAAGAAGACATCTCGTCGTCGCATCACAT
TACCTGAACACTTAGCACGAGAAGAATACCGCTTAGATACGCATGAGTCCTGCCCAGACTGCCAAGGTGCGTTGTGCCATATCGGTGATGATGTCTCAGA
AATCTTGGATGTGGTGCCAGCATCGTATCGTGTGATTAAAACCGTGCGACCTAAATACAGCTGTAAGGCCTGTGACACCGTGGTTCAAGCGCCTGCACCG
CAACGAGTGATTAATAAAGGCCTGCCAAGTGCTGGGTTGTTGACACAAGTGATGGTCGATAAATACATGGACCATCAGCCGTTGTACCGTCAATCACAAC
AGATGGCGCGAGAAGGGATTGATGTTGAGCGCTCAACGTTAGCGGATTGGGTGGGGCAAGTGAGTCGCTTACTCACTCCGCTAGCGCAAGCAATAGAGCG
TCATGTCACACAGGCTTCTCACTTACATGCCGATGACACCACAGCACCGACACTATCACCAGGTAAAGGACGTACACAAACGGGACGATATTGGACGTAT
GTTAGGGATGGTCGAGCATGGAATGAAGCTGCGCCGCCTGCGGTCTGGTTACAATACAGTGAAGATAGAAAAAGTATCCATCCCACCATGCATTTACGCC
ACTTTACTGGGGCACTGCAAGCGGATGCGTATGCTGGTTATAATGATGTGTATCGTAACAAGGTGACTTTTGTCGCTTGTTTAGCGCATGTCAGGCGAAA
GTTTTATGATATTACCCAATCGGGCCCAGCGCCTACTGCACAGCAGGCTATCGAATATATCCAACAGCTGTATGCGATTGAAAAAACTATCCGCAATCAA
CCACCGGATAAGCGCTTAGCAATACGACAACAACAATCTGTGCCGATACTGACTGAGTTTTATGATTACTTAGCCACAAGCTTGCGCACCTTATCAAAAG
GCTCGGCGATGTCTAAAGCCGTGGCGTATGCGATAAATCAACAAGATGGGATTATGGCCTATACCCAAGATGGTCGTGCGGCTATTGATAACAACTCGGC
GGAGCGGTCTATCCGTCCCATTGCACTGGGTAGAAAAAATTACTTGTTCGCAGGCTCTAAAGCCGGCGGTGAACGAGCTGCAGTACTGTATACCATTCTC
GGTACAGCGAGATTAAATGATATTAATCCGAATCAATATCTCACAGCAGTCTTAAAGCGCATAGGACAACACCAAATCAATAAAGTCGATGAACTCTTAC
CATGGAACATTGACCTAAGCACACCCACCCAAAACGAAGCTATCTAAATCAGAAGACTAAGTGTCCACTAAAAAACAAGTGGACACTTAAATGAAGATAC
TGGGTGTAGTGGCCGGACGGTTAC
CTAGTCGCCGGATCTTCTCGCCTCAATATAAATTAGAGATCGTGAATGAGGCGTTAACCAAGCAGTTATCAACTGCCCAAATTGCACAAAAGCACCACAT
CAATAACAACCAAGTTTTTCACTGGTGTCGCGAATATAAACTTGGTGTGGCAAGATGGGTAAAAGTAGCAAGCAACAACCCTGTGATTGCGACACAAGAT
CCCCCTGGAGAGACCAAGTTTTTACCCATCACGACTCAATCCAGCTCCAATACTGTCATGCGCAATGATCAACAACAGACGCCAAACTCAAGTACGGTGG
TCGTTGAGCTAGCCAGTGGGCACGTGCTCAAGCTCACACATCCCACGGCTGAGACGATCAAACAGATCTTATTGGTTATCGTATGATCACTTTATCTCAT
GATGTACGGATCTGGTTATGTGCCGGCCACACCGATATGCGTAAAGGCTTTGATAGTTTATCTGCGATTGCGCAGCATATATTACAAAAAGATCCCTTTA
ATGGGCATGTTTTTGTGTTTCGCGGCAAACGTGGTGATCGGGTTAAATTACTATGGTGGGATGGCCAAGGATTATGTCTGTATTATAAACGTTTGGAGCA
CGGACGTTTTGTGTGGCCGAGCGCAACATCCGGTGCTGTTCACTTATCACAAGCCCAATTATCCATGCTATTAGAGGGCATTGATTGGCGCCCACCGGCA
AGGCGATATCGACCGGGAAATAGTGCGTAAAAAACCGTGAATTTATTGCTTTATCCCGTCTTTTTATTGGGCTTGAGTGGTATAATTAAGCTCATGAAAA
CAGGCCGTCAAGCACTTCCAACCGATACTCATGCATTGCAACAAATGGTGTTGCAGATGCAATCTGAGCTGTCTGAAAATAATAAGAAACTGTCCGCTCA
AGACTCCGTTATCGATAACCTACGCCATCAATTAGCGGTATTAAAGCGTGCGCGTTTTGGTCGATCATCAGAGCAAGTTGAGCAAAATATCCATCAGTTG
GAATTACAGCTTGAAGAGCTAGAAATCGCCCAAGCACAGACGGCCCCTGGTAGCCGTGCTGCAGGCACGGTCAAGAAGACATCTCGTCGTCGCATCACAT
TACCTGAACACTTAGCACGAGAAGAATACCGCTTAGATACGCATGAGTCCTGCCCAGACTGCCAAGGTGCGTTGTGCCATATCGGTGATGATGTCTCAGA
AATCTTGGATGTGGTGCCAGCATCGTATCGTGTGATTAAAACCGTGCGACCTAAATACAGCTGTAAGGCCTGTGACACCGTGGTTCAAGCGCCTGCACCG
CAACGAGTGATTAATAAAGGCCTGCCAAGTGCTGGGTTGTTGACACAAGTGATGGTCGATAAATACATGGACCATCAGCCGTTGTACCGTCAATCACAAC
AGATGGCGCGAGAAGGGATTGATGTTGAGCGCTCAACGTTAGCGGATTGGGTGGGGCAAGTGAGTCGCTTACTCACTCCGCTAGCGCAAGCAATAGAGCG
TCATGTCACACAGGCTTCTCACTTACATGCCGATGACACCACAGCACCGACACTATCACCAGGTAAAGGACGTACACAAACGGGACGATATTGGACGTAT
GTTAGGGATGGTCGAGCATGGAATGAAGCTGCGCCGCCTGCGGTCTGGTTACAATACAGTGAAGATAGAAAAAGTATCCATCCCACCATGCATTTACGCC
ACTTTACTGGGGCACTGCAAGCGGATGCGTATGCTGGTTATAATGATGTGTATCGTAACAAGGTGACTTTTGTCGCTTGTTTAGCGCATGTCAGGCGAAA
GTTTTATGATATTACCCAATCGGGCCCAGCGCCTACTGCACAGCAGGCTATCGAATATATCCAACAGCTGTATGCGATTGAAAAAACTATCCGCAATCAA
CCACCGGATAAGCGCTTAGCAATACGACAACAACAATCTGTGCCGATACTGACTGAGTTTTATGATTACTTAGCCACAAGCTTGCGCACCTTATCAAAAG
GCTCGGCGATGTCTAAAGCCGTGGCGTATGCGATAAATCAACAAGATGGGATTATGGCCTATACCCAAGATGGTCGTGCGGCTATTGATAACAACTCGGC
GGAGCGGTCTATCCGTCCCATTGCACTGGGTAGAAAAAATTACTTGTTCGCAGGCTCTAAAGCCGGCGGTGAACGAGCTGCAGTACTGTATACCATTCTC
GGTACAGCGAGATTAAATGATATTAATCCGAATCAATATCTCACAGCAGTCTTAAAGCGCATAGGACAACACCAAATCAATAAAGTCGATGAACTCTTAC
CATGGAACATTGACCTAAGCACACCCACCCAAAACGAAGCTATCTAAATCAGAAGACTAAGTGTCCACTAAAAAACAAGTGGACACTTAAATGAAGATAC
TGGGTGTAGTGGCCGGACGGTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 408 bp | 135 aa | 79 | 486 | + | No |
AG : IS66 TnpA
ORF sequence :
MSDIIHHTSRRIFSPQYKLEIVNEALTKQLSTAQIAQKHHINNNQVFHWCREYKLGVARWVKVASNNPVIATQDPPGETKFLPITTQSSSNTVMRNDQQQ
TPNSSTVVVELASGHVLKLTHPTAETIKQILLVIV
TPNSSTVVVELASGHVLKLTHPTAETIKQILLVIV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 483 | 830 | + | No |
AG : IS66 TnpB
ORF sequence :
MITLSHDVRIWLCAGHTDMRKGFDSLSAIAQHILQKDPFNGHVFVFRGKRGDRVKLLWWDGQGLCLYYKRLEHGRFVWPSATSGAVHLSQAQLSMLLEGI
DWRPPARRYRPGNSA
DWRPPARRYRPGNSA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1554 bp | 517 aa | 894 | 2447 | + | No |
Chemistry : DDE
ORF sequence :
MKTGRQALPTDTHALQQMVLQMQSELSENNKKLSAQDSVIDNLRHQLAVLKRARFGRSSEQVEQNIHQLELQLEELEIAQAQTAPGSRAAGTVKKTSRRR
ITLPEHLAREEYRLDTHESCPDCQGALCHIGDDVSEILDVVPASYRVIKTVRPKYSCKACDTVVQAPAPQRVINKGLPSAGLLTQVMVDKYMDHQPLYRQ
SQQMAREGIDVERSTLADWVGQVSRLLTPLAQAIERHVTQASHLHADDTTAPTLSPGKGRTQTGRYWTYVRDGRAWNEAAPPAVWLQYSEDRKSIHPTMH
LRHFTGALQADAYAGYNDVYRNKVTFVACLAHVRRKFYDITQSGPAPTAQQAIEYIQQLYAIEKTIRNQPPDKRLAIRQQQSVPILTEFYDYLATSLRTL
SKGSAMSKAVAYAINQQDGIMAYTQDGRAAIDNNSAERSIRPIALGRKNYLFAGSKAGGERAAVLYTILGTARLNDINPNQYLTAVLKRIGQHQINKVDE
LLPWNIDLSTPTQNEAI
ITLPEHLAREEYRLDTHESCPDCQGALCHIGDDVSEILDVVPASYRVIKTVRPKYSCKACDTVVQAPAPQRVINKGLPSAGLLTQVMVDKYMDHQPLYRQ
SQQMAREGIDVERSTLADWVGQVSRLLTPLAQAIERHVTQASHLHADDTTAPTLSPGKGRTQTGRYWTYVRDGRAWNEAAPPAVWLQYSEDRKSIHPTMH
LRHFTGALQADAYAGYNDVYRNKVTFVACLAHVRRKFYDITQSGPAPTAQQAIEYIQQLYAIEKTIRNQPPDKRLAIRQQQSVPILTEFYDYLATSLRTL
SKGSAMSKAVAYAINQQDGIMAYTQDGRAAIDNNSAERSIRPIALGRKNYLFAGSKAGGERAAVLYTILGTARLNDINPNQYLTAVLKRIGQHQINKVDE
LLPWNIDLSTPTQNEAI
Blast result :
Comments
ISSpu24 is 29% (ORFA) identical to ISSfl3_aa1, 66% (ORFB) to ISPre3_aa2, and 48% (ORFC, the transposase) to ISCARN48_aa3.
References
1] Romine M. (2010) Direct submission.