ISAmi2
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013093 | ND | Actinosynnema mirum | Actinosynnema mirum DSM 43827 |
DNA section
IS Length : 942 bp
Ends
IR Length : 20/23
IRL : GAGCCCTTTTCATCCTGGGCTGGATGCGTTGCCGTGTAGTGCTTCTTGCT
IRR : GAGCCTTTTTCATCCTGTGGTGGCCTCGTAGTTCTGTAGGACGTGGATGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGAAAGTTC | AGT | GAGCGGGGCG | 3 |
| GACATCGTCC | AGT | GCGTCCAGCA | 3 |
| CTGCGCCTGC | ACT | CAGGCCTCCC | 3 |
| GTACTGGGCC | ATT | GCCTGGGCGT | 3 |
| CCAAGATCCT | ACTGAACCGA | 0 |
DNA sequence
GAGCCCTTTTCATCCTGGGCTGGATGCGTTGCCGTGTAGTGCTTCTTGCTGATCACAGGTTGATGAGCATCGGCGTGGCTCCGCCCGGACATGGCGAAGC
CCCTGGTAGCAGAGCTATCGACCAAGAAAGTTCTGCACCGCCAGGGGCTTCACGTGCTGTTCTACCGTGCCGCGCTGCCGTTGTCACATCAGACCCTGAC
CTTCGTGTCCGGGCTGGTCCGCGCCCACCGCAAGCAGCTCGGATCGGTGTGGCGCAAGCTCAACCCAGGACAACAAGCCCTGCTGGTGCTGGTGTACCTG
CGCAAAGGCGAGACCTTCGCCGATCTCGCCGCCGGGTTCGCGGTCTCGACCTCCACCTGCTGGCGCTATGTCAACGAGACCGTCGAACTGCTCGCCCAGC
GGTCCCCGAAGCTCCGAGAAGCGCTGCGCAAGGCGAAACGCCAGGGCATGGCCCACGTGGTGATCGACGGCACGCTCATCCCGATCGACCGGATCGCCGC
CGACCGCCCCTTCTACTCCGGCAAGCACAAGATCCATGGAGTGAACCTGCAGGTGATCGCGTCCCCGGACGGCACGATCCTGTGGGTGTCCGGCGACTTG
CCCGGCAGCACCCACGACACCGCCGCCGCCCGGATCTGGAACATCCTCGCCGCCCTGCGCCACGCCGGGCTGATCGCCCTGGGCGACAAGGGCTACCACG
CCTACGACCCGACCGACCGCCACGTGATCACCCCTTACAAGGGCCGCAACAAACCTGAATCACAGAAGGACGCCAACCGCGCCCATGCCCGCCTGCGCGG
CCCCGGCGAACGCGCCAACGCCCAGCTCAAGACCTGGCGCATCCTCCGCAAACTCCGCTGCTGCCCCCGCCGCGCCGGCCGACTGGCCAAAGCCATCCAC
GTCCTACAGAACTACGAGGCCACCACAGGATGAAAAAGGCTC
CCCTGGTAGCAGAGCTATCGACCAAGAAAGTTCTGCACCGCCAGGGGCTTCACGTGCTGTTCTACCGTGCCGCGCTGCCGTTGTCACATCAGACCCTGAC
CTTCGTGTCCGGGCTGGTCCGCGCCCACCGCAAGCAGCTCGGATCGGTGTGGCGCAAGCTCAACCCAGGACAACAAGCCCTGCTGGTGCTGGTGTACCTG
CGCAAAGGCGAGACCTTCGCCGATCTCGCCGCCGGGTTCGCGGTCTCGACCTCCACCTGCTGGCGCTATGTCAACGAGACCGTCGAACTGCTCGCCCAGC
GGTCCCCGAAGCTCCGAGAAGCGCTGCGCAAGGCGAAACGCCAGGGCATGGCCCACGTGGTGATCGACGGCACGCTCATCCCGATCGACCGGATCGCCGC
CGACCGCCCCTTCTACTCCGGCAAGCACAAGATCCATGGAGTGAACCTGCAGGTGATCGCGTCCCCGGACGGCACGATCCTGTGGGTGTCCGGCGACTTG
CCCGGCAGCACCCACGACACCGCCGCCGCCCGGATCTGGAACATCCTCGCCGCCCTGCGCCACGCCGGGCTGATCGCCCTGGGCGACAAGGGCTACCACG
CCTACGACCCGACCGACCGCCACGTGATCACCCCTTACAAGGGCCGCAACAAACCTGAATCACAGAAGGACGCCAACCGCGCCCATGCCCGCCTGCGCGG
CCCCGGCGAACGCGCCAACGCCCAGCTCAAGACCTGGCGCATCCTCCGCAAACTCCGCTGCTGCCCCCGCCGCGCCGGCCGACTGGCCAAAGCCATCCAC
GTCCTACAGAACTACGAGGCCACCACAGGATGAAAAAGGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 780 bp | 259 aa | 154 | 933 | + | No |
Chemistry : DDE
ORF sequence :
MLFYRAALPLSHQTLTFVSGLVRAHRKQLGSVWRKLNPGQQALLVLVYLRKGETFADLAAGFAVSTSTCWRYVNETVELLAQRSPKLREALRKAKRQGMA
HVVIDGTLIPIDRIAADRPFYSGKHKIHGVNLQVIASPDGTILWVSGDLPGSTHDTAAARIWNILAALRHAGLIALGDKGYHAYDPTDRHVITPYKGRNK
PESQKDANRAHARLRGPGERANAQLKTWRILRKLRCCPRRAGRLAKAIHVLQNYEATTG
HVVIDGTLIPIDRIAADRPFYSGKHKIHGVNLQVIASPDGTILWVSGDLPGSTHDTAAARIWNILAALRHAGLIALGDKGYHAYDPTDRHVITPYKGRNK
PESQKDANRAHARLRGPGERANAQLKTWRILRKLRCCPRRAGRLAKAIHVLQNYEATTG
Blast result :
Comments
ISAmi2 is 62% aa similar to ISSro1.
References
1] ISfinder annotation (2010).
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.