ISAmde1
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013385 | ND | Ammonifex degensii | Ammonifex degensii KC4 |
DNA section
IS Length : 1827 bp
Ends
IR Length : 14/15
IRL : GGGGTTGTTGAAAAACAAATTGCGACAAACAAGAAAGTTGATTAAACCAT
IRR : GGGGCTGTTGAAAAAGGCCTTCTTTCTACCTTGGACTAAGCTATGGCCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCCCCGGGC | TTAA | AGGAGGGGGA | 4 |
| TTCCCCTGAC | TGAA | GTCAGGGGCT | 4 |
| CTCCCCAGGG | TTAAA | AGCCGGGGCC | 5 |
DNA sequence
GGGGTTGTTGAAAAACAAATTGCGACAAACAAGAAAGTTGATTAAACCATGAGGCATTTATGTTGTAGTTACGGTCGTGTTGTGGTACATTAGTGTTGTA
GGTTGTTTTGAAGGGATGAGAGTAGATGATGGGTAAAAAAGTACGTCAGTGTTCTTTGGTTGCTGGCACAGCCTTATCCCAGAAAACCACTTTCTGCGCC
AGGTAGAAAGGGTAGTCGACTTCTCCTTCATCCGGAAAAAGGTGGCTCATCTGTATTCCCACACCGGCCGGCCTTCCATTGACCCCGAAGTGCTTATCCG
GATACTGCTCATAGGCTATTTTTACGGCATTACTTCCGAACGCGAACTAATGGAGCAGATTCGGGTCAACCTGGCTTTCCGGGAATTCATAGGGTACGAG
CTGGACGAGGAGATTCCCGACCATTCCACCCTGTCGAAGAACCGCCACGGCCGTTTCAAGGGTACTTCTGTCTTTCAGGAGATTTTTGATGAGATTGTTC
GGCAGTGCATGGCAGCGGGTCTAGTAGCCGGCAAGCACCTTTCTTTTGACGGTACTACTATTCAGGCCAACGCTTCCTACGATTCCATGGTACCCAGGCG
GGTAGTTGAGATGTCCCCTGCGGAGTACATCCAAAAAGTAGAACAGGAAAATCCTGTGACACCAGAGGTGGAAGAAGAGCAGCAAATGTCCATGGAAGAG
GGAACTACACTAGATGATTCTGGAACACCTCCCGGCTCGAATGGAAACTTCTGTTCTGTTGCGTCCGGGCCAGAGGATGCCTTGGTAACAGACGACACTG
CGGATACCCCGCAAGAGGGCTTGAGTTTTGCTTCCAAAGCCGGTGGCGGCAAAAAGATGTCGGGCAAGTCAAGAAGGTCAGAGAAGAAGACCAACGCTAC
TCACATAAGCCGCACTGACCCGGACGCCAGGATATTTGCCCGTCCCGGGCAACCGAAAAAGCTGGGCTACATCGAGTGTGTTACAGTAGATAGCCTGTAC
AACATTATCACTGGGGTTGAAGTAATTCCCGGCAACGCTGATGAGGCCCGAGTACTGGTTCCCATGATAACTTCCCAGATGTTCAAGTTTGGCTTTCGGG
TGGACTCCGTATCGGGTGACAAGGCCCACGGTGAATCACGCGTCTACAAAGAACTTCTGGCGCTGGGCATCAAACCTTTCATTCCCCATCAAAACTTTCA
TGAAACCAACCGAAACATCTGGCCCCAGGAAAAGTTCCGGTATGATGCCAAGGAAGACTGCTATATTTGCCCTAACGGCAAAGTTCTCCGGTATCAAGGG
TGTATAGACCGCGGGATTATGAAGGTGTACCGGACAAGGCCCAGAGACTGTAAGGATTGCCCGTATAAATCTCAGTGTACCACTTCTGAGAAGGTAGGTC
GGACTATCAAACGGCATGTAGACGCACGGGTGTTTGAATACGTTGATCGGCTACTCAAGCGCCCTGAAGCCCGGGAGGCTGCAATCTGGCGGCGTACTGG
ACCCGAGACTGTATTTGGGGAAGCTAAGGAATACCACGGGCTTAGGAGGGCTAAGTTTAGAGGTCTTGAACATGTTAAAGAACAGTGCCTGCTAACTGCT
ACGGCCCAGAACATAAAGCGTATGGTCAAGGCATTAACAAGGAGAAAACCTCCCAAAGTAAGGGAGGCTGCTATGCCTGCTAGAACCCTAAAAGCCAACC
AGGTTCAAACCAGGTCGAGTTTTTCTTTGATTCAGTCAAACTTAACAGCTTTGTGCTCCGGCCCGATTGTTTTTCAGCGGGCCATAGCTTAGTCCAAGGT
AGAAAGAAGGCCTTTTTCAACAGCCCC
GGTTGTTTTGAAGGGATGAGAGTAGATGATGGGTAAAAAAGTACGTCAGTGTTCTTTGGTTGCTGGCACAGCCTTATCCCAGAAAACCACTTTCTGCGCC
AGGTAGAAAGGGTAGTCGACTTCTCCTTCATCCGGAAAAAGGTGGCTCATCTGTATTCCCACACCGGCCGGCCTTCCATTGACCCCGAAGTGCTTATCCG
GATACTGCTCATAGGCTATTTTTACGGCATTACTTCCGAACGCGAACTAATGGAGCAGATTCGGGTCAACCTGGCTTTCCGGGAATTCATAGGGTACGAG
CTGGACGAGGAGATTCCCGACCATTCCACCCTGTCGAAGAACCGCCACGGCCGTTTCAAGGGTACTTCTGTCTTTCAGGAGATTTTTGATGAGATTGTTC
GGCAGTGCATGGCAGCGGGTCTAGTAGCCGGCAAGCACCTTTCTTTTGACGGTACTACTATTCAGGCCAACGCTTCCTACGATTCCATGGTACCCAGGCG
GGTAGTTGAGATGTCCCCTGCGGAGTACATCCAAAAAGTAGAACAGGAAAATCCTGTGACACCAGAGGTGGAAGAAGAGCAGCAAATGTCCATGGAAGAG
GGAACTACACTAGATGATTCTGGAACACCTCCCGGCTCGAATGGAAACTTCTGTTCTGTTGCGTCCGGGCCAGAGGATGCCTTGGTAACAGACGACACTG
CGGATACCCCGCAAGAGGGCTTGAGTTTTGCTTCCAAAGCCGGTGGCGGCAAAAAGATGTCGGGCAAGTCAAGAAGGTCAGAGAAGAAGACCAACGCTAC
TCACATAAGCCGCACTGACCCGGACGCCAGGATATTTGCCCGTCCCGGGCAACCGAAAAAGCTGGGCTACATCGAGTGTGTTACAGTAGATAGCCTGTAC
AACATTATCACTGGGGTTGAAGTAATTCCCGGCAACGCTGATGAGGCCCGAGTACTGGTTCCCATGATAACTTCCCAGATGTTCAAGTTTGGCTTTCGGG
TGGACTCCGTATCGGGTGACAAGGCCCACGGTGAATCACGCGTCTACAAAGAACTTCTGGCGCTGGGCATCAAACCTTTCATTCCCCATCAAAACTTTCA
TGAAACCAACCGAAACATCTGGCCCCAGGAAAAGTTCCGGTATGATGCCAAGGAAGACTGCTATATTTGCCCTAACGGCAAAGTTCTCCGGTATCAAGGG
TGTATAGACCGCGGGATTATGAAGGTGTACCGGACAAGGCCCAGAGACTGTAAGGATTGCCCGTATAAATCTCAGTGTACCACTTCTGAGAAGGTAGGTC
GGACTATCAAACGGCATGTAGACGCACGGGTGTTTGAATACGTTGATCGGCTACTCAAGCGCCCTGAAGCCCGGGAGGCTGCAATCTGGCGGCGTACTGG
ACCCGAGACTGTATTTGGGGAAGCTAAGGAATACCACGGGCTTAGGAGGGCTAAGTTTAGAGGTCTTGAACATGTTAAAGAACAGTGCCTGCTAACTGCT
ACGGCCCAGAACATAAAGCGTATGGTCAAGGCATTAACAAGGAGAAAACCTCCCAAAGTAAGGGAGGCTGCTATGCCTGCTAGAACCCTAAAAGCCAACC
AGGTTCAAACCAGGTCGAGTTTTTCTTTGATTCAGTCAAACTTAACAGCTTTGTGCTCCGGCCCGATTGTTTTTCAGCGGGCCATAGCTTAGTCCAAGGT
AGAAAGAAGGCCTTTTTCAACAGCCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1643 bp | 547 aa | 150 | 1792 | + | No |
Chemistry : DDE
ORF sequence :
MFFGCWHSLIPENHFLRQVERVVDFSFIRKKVAHLYSHTGRPSIDPEVLIRILLIGYFYGITSERELMEQIRVNLAFREFIGYELDEEIPDHSTLSKNRH
GRFKGTSVFQEIFDEIVRQCMAAGLVAGKHLSFDGTTIQANASYDSMVPRRVVEMSPAEYIQKVEQENPVTPEVEEEQQMSMEEGTTLDDSGTPPGSNGN
FCSVASGPEDALVTDDTADTPQEGLSFASKAGGGKKMSGKSRRSEKKTNATHISRTDPDARIFARPGQPKKLGYIECVTVDSLYNIITGVEVIPGNADEA
RVLVPMITSQMFKFGFRVDSVSGDKAHGESRVYKELLALGIKPFIPHQNFHETNRNIWPQEKFRYDAKEDCYICPNGKVLRYQGCIDRGIMKVYRTRPRD
CKDCPYKSQCTTSEKVGRTIKRHVDARVFEYVDRLLKRPEAREAAIWRRTGPETVFGEAKEYHGLRRAKFRGLEHVKEQCLLTATAQNIKRMVKALTRRK
PPKVREAAMPARTLKANQVQTRSSFSLIQSNLTALCSGPIVFQRAIA
GRFKGTSVFQEIFDEIVRQCMAAGLVAGKHLSFDGTTIQANASYDSMVPRRVVEMSPAEYIQKVEQENPVTPEVEEEQQMSMEEGTTLDDSGTPPGSNGN
FCSVASGPEDALVTDDTADTPQEGLSFASKAGGGKKMSGKSRRSEKKTNATHISRTDPDARIFARPGQPKKLGYIECVTVDSLYNIITGVEVIPGNADEA
RVLVPMITSQMFKFGFRVDSVSGDKAHGESRVYKELLALGIKPFIPHQNFHETNRNIWPQEKFRYDAKEDCYICPNGKVLRYQGCIDRGIMKVYRTRPRD
CKDCPYKSQCTTSEKVGRTIKRHVDARVFEYVDRLLKRPEAREAAIWRRTGPETVFGEAKEYHGLRRAKFRGLEHVKEQCLLTATAQNIKRMVKALTRRK
PPKVREAAMPARTLKANQVQTRSSFSLIQSNLTALCSGPIVFQRAIA
Blast result :
Comments
ISAmde1 is 50% aa similar to ISOih1.
References
1] ISfinder annotation (2010)
2] Kerfeld,C., Goodner,B., Huber,H., Stetter,K., Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Richardson,P. (2009) Direct submission GenBank.
2] Kerfeld,C., Goodner,B., Huber,H., Stetter,K., Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Richardson,P. (2009) Direct submission GenBank.