ISBpu1
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009848 | ND | Bacillus pumilus | Bacillus pumilus SAFR-032 |
DNA section
IS Length : 1709 bp
Ends
IR Length : 14/15
IRL : CAGCTTGTAGACAAAGTCTACAAGCTTTTTTGTATAATAAGGGTAATAAC
IRR : CAGCGTGTAGACAAACCCTCGCATTCGTTGTCAGTCCTGCGTGCTGGTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACGTACGTAA | AGAGAGCTCACCTATTAAGGTGAGCTCA/T | CTTTCTTGAT | 30 |
DNA sequence
CAGCTTGTAGACAAAGTCTACAAGCTTTTTTGTATAATAAGGGTAATAACAATAATCTGTGGGGGATAAACATGTTATCGAAACGTGAAGAAGAAAGAAG
AAATCAATTAGAAGTAGTGGCTTTAGATGAATTAGTGCCTGAAGATCATCTTGTGCGGAAAATTGAACAAGCAATCGATTTCGATTTCATTTATGATCTT
GTCAAAGATCATTATAGTTCTGATAATGGTAGACCAAGTGTTGATCCTGTTGTTCTCATTAAGATGGTCTTAATCCAATATCTATTTGGCATTCGCTCCA
TGCGTCAAACCATTAAAGAAATTGAAACCAATGTGGCTTATCGTTGGTTTATTGGGTATGGATTTTCGGAGAAAATTCCGCACTTTTCTACCTTCGGTAA
AAACTACGTGAGACGCTTCCACGATACAGATTTATTCGAACAAATTTTTTATCGAATCTTAAGAGAAGCGATGAAAAAAGGGCTCGTTGACCCGTCTATC
GCCTTCATTGATTCGACTCATGTCAAAGCGAACGCCAATAAGAAAAAACTTGAAAAGAAAATCGTTCGAGTTGAGACAAGAAGTTATCAAAATCAGCTTC
ATGAAGAGATCAATATTGATCGTGAAGAACATGGAAAAAAGCCCTTACCCCTACAGAATAAAGAAGAAACAAAAGAAATCAAGGTCAGTACAACTGACCC
AGAGAGTGGCTATTATGTAAAGGATGAACGTGAAAAGTCATTTGCTTATTCATTTCATACAGCGTGTGATTCAAAAGGATTTATTTTAGGCTCGCTGGTC
ACAGGCGGAAATGTTCATGATAGTCGTATGTTGGATTCACTCGTATCAAAAGTGACAGAGAAAGTCGGAAAACCAAATGTAGTCGCAGTAGATGCGGGGT
ATAAAACACCTTACATCGCTAAGTTCTTAATGGATCAAGAGATAAGACCTGTCATGCCCTACACTCGTCCAAGAACAAAAGCTGGTTATTTGAAAAAGAA
TGAATACGTATATGATGCATATTTTGATTGTTACATTTGCCCCAACGGGCAAATTCTCTCTTATCGTACAACGACTAGAGAAGGCTATAAGCAATATGTA
TCCGATTCAGAAGTATGTGAGTCCTGCTCGTTTTTAGATAACTGTACCCAAAGTCAAAACCACACCAAACAAATTCATCGGCATATTTGGCAGGATTATC
TAGATGAAGCAGACAATCTGAGACTCACTGAAATAAATAAGAGCATCTACGCAAAACGGAAAGAAACGATTGAACGCGTTTTTGCGGATGCAAAAGAAAA
GCATGGTATGCGTTGGACGAAACTTCGTGGACTAAAAAAAGTTTCAATGCAAGCGATGCTTACTTTCGCTGCCATGAACCTTAAAAAACTAGCAAACTGG
ACATGGAAAGTACCATGTCCAGCGTAAAAACACATGATGTTTTTCAAAAAAAAATGGTGAAACCCCCACTTTTTTCTCGAAGTTAAGGTAGGGTTTGTCT
CCTGTCTGAGACCTCCTATTAAGGTGAGCTCTCTCAGATTGTTGACAAAGGGCTAAAATGATCTTTATTTTAGCCCTTTGTCTCCTTTTCAGCGTGATAG
AAAACCTTTGAATTCTAGGAAGGACGAGTACTGGCGCGGAGCGAATTTGACATTCGTGAGCACCAGCACGCAGGACTGACAACGAATGCGAGGGTTTGTC
TACACGCTG
AAATCAATTAGAAGTAGTGGCTTTAGATGAATTAGTGCCTGAAGATCATCTTGTGCGGAAAATTGAACAAGCAATCGATTTCGATTTCATTTATGATCTT
GTCAAAGATCATTATAGTTCTGATAATGGTAGACCAAGTGTTGATCCTGTTGTTCTCATTAAGATGGTCTTAATCCAATATCTATTTGGCATTCGCTCCA
TGCGTCAAACCATTAAAGAAATTGAAACCAATGTGGCTTATCGTTGGTTTATTGGGTATGGATTTTCGGAGAAAATTCCGCACTTTTCTACCTTCGGTAA
AAACTACGTGAGACGCTTCCACGATACAGATTTATTCGAACAAATTTTTTATCGAATCTTAAGAGAAGCGATGAAAAAAGGGCTCGTTGACCCGTCTATC
GCCTTCATTGATTCGACTCATGTCAAAGCGAACGCCAATAAGAAAAAACTTGAAAAGAAAATCGTTCGAGTTGAGACAAGAAGTTATCAAAATCAGCTTC
ATGAAGAGATCAATATTGATCGTGAAGAACATGGAAAAAAGCCCTTACCCCTACAGAATAAAGAAGAAACAAAAGAAATCAAGGTCAGTACAACTGACCC
AGAGAGTGGCTATTATGTAAAGGATGAACGTGAAAAGTCATTTGCTTATTCATTTCATACAGCGTGTGATTCAAAAGGATTTATTTTAGGCTCGCTGGTC
ACAGGCGGAAATGTTCATGATAGTCGTATGTTGGATTCACTCGTATCAAAAGTGACAGAGAAAGTCGGAAAACCAAATGTAGTCGCAGTAGATGCGGGGT
ATAAAACACCTTACATCGCTAAGTTCTTAATGGATCAAGAGATAAGACCTGTCATGCCCTACACTCGTCCAAGAACAAAAGCTGGTTATTTGAAAAAGAA
TGAATACGTATATGATGCATATTTTGATTGTTACATTTGCCCCAACGGGCAAATTCTCTCTTATCGTACAACGACTAGAGAAGGCTATAAGCAATATGTA
TCCGATTCAGAAGTATGTGAGTCCTGCTCGTTTTTAGATAACTGTACCCAAAGTCAAAACCACACCAAACAAATTCATCGGCATATTTGGCAGGATTATC
TAGATGAAGCAGACAATCTGAGACTCACTGAAATAAATAAGAGCATCTACGCAAAACGGAAAGAAACGATTGAACGCGTTTTTGCGGATGCAAAAGAAAA
GCATGGTATGCGTTGGACGAAACTTCGTGGACTAAAAAAAGTTTCAATGCAAGCGATGCTTACTTTCGCTGCCATGAACCTTAAAAAACTAGCAAACTGG
ACATGGAAAGTACCATGTCCAGCGTAAAAACACATGATGTTTTTCAAAAAAAAATGGTGAAACCCCCACTTTTTTCTCGAAGTTAAGGTAGGGTTTGTCT
CCTGTCTGAGACCTCCTATTAAGGTGAGCTCTCTCAGATTGTTGACAAAGGGCTAAAATGATCTTTATTTTAGCCCTTTGTCTCCTTTTCAGCGTGATAG
AAAACCTTTGAATTCTAGGAAGGACGAGTACTGGCGCGGAGCGAATTTGACATTCGTGAGCACCAGCACGCAGGACTGACAACGAATGCGAGGGTTTGTC
TACACGCTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1356 bp | 451 aa | 72 | 1427 | + | No |
Chemistry : DDE
ORF sequence :
MLSKREEERRNQLEVVALDELVPEDHLVRKIEQAIDFDFIYDLVKDHYSSDNGRPSVDPVVLIKMVLIQYLFGIRSMRQTIKEIETNVAYRWFIGYGFSE
KIPHFSTFGKNYVRRFHDTDLFEQIFYRILREAMKKGLVDPSIAFIDSTHVKANANKKKLEKKIVRVETRSYQNQLHEEINIDREEHGKKPLPLQNKEET
KEIKVSTTDPESGYYVKDEREKSFAYSFHTACDSKGFILGSLVTGGNVHDSRMLDSLVSKVTEKVGKPNVVAVDAGYKTPYIAKFLMDQEIRPVMPYTRP
RTKAGYLKKNEYVYDAYFDCYICPNGQILSYRTTTREGYKQYVSDSEVCESCSFLDNCTQSQNHTKQIHRHIWQDYLDEADNLRLTEINKSIYAKRKETI
ERVFADAKEKHGMRWTKLRGLKKVSMQAMLTFAAMNLKKLANWTWKVPCPA
KIPHFSTFGKNYVRRFHDTDLFEQIFYRILREAMKKGLVDPSIAFIDSTHVKANANKKKLEKKIVRVETRSYQNQLHEEINIDREEHGKKPLPLQNKEET
KEIKVSTTDPESGYYVKDEREKSFAYSFHTACDSKGFILGSLVTGGNVHDSRMLDSLVSKVTEKVGKPNVVAVDAGYKTPYIAKFLMDQEIRPVMPYTRP
RTKAGYLKKNEYVYDAYFDCYICPNGQILSYRTTTREGYKQYVSDSEVCESCSFLDNCTQSQNHTKQIHRHIWQDYLDEADNLRLTEINKSIYAKRKETI
ERVFADAKEKHGMRWTKLRGLKKVSMQAMLTFAAMNLKKLANWTWKVPCPA
Blast result :
Comments
ISBpu1 is 82% aa similar to ISBcl1.
References
1] ISfinder (2010)
2] Gioia,J., Yerrapragada,S., Qin,X., Jiang,H., Igboeli,O.C., Muzny,D., Dugan-Rocha,S., Ding,Y., Hawes,A., Liu,W., Perez,L., Kovar,C., Dinh,H., Lee,S., Nazareth,L., Blyth,P., Holder,M., Buhay,C., Tirumalai,M.R., Liu,Y., Dasgupta,I., Bokhetache,L., Fujita,M., Karouia,F., Eswara Moorthy,P., Siefert,J., Uzman,A., Buzumbo,P., Verma,A., Zwiya,H., McWilliams,B.D., Olowu,A., Clinkenbeard,K.D., Newcombe,D., Golebiewski,L., Petrosino,J.F., Nicholson,W.L., Fox,G.E., Venkateswaran,K., Highlander,S.K. and Weinstock,G.M. (2007) PLoS ONE 2 (9), E928.
2] Gioia,J., Yerrapragada,S., Qin,X., Jiang,H., Igboeli,O.C., Muzny,D., Dugan-Rocha,S., Ding,Y., Hawes,A., Liu,W., Perez,L., Kovar,C., Dinh,H., Lee,S., Nazareth,L., Blyth,P., Holder,M., Buhay,C., Tirumalai,M.R., Liu,Y., Dasgupta,I., Bokhetache,L., Fujita,M., Karouia,F., Eswara Moorthy,P., Siefert,J., Uzman,A., Buzumbo,P., Verma,A., Zwiya,H., McWilliams,B.D., Olowu,A., Clinkenbeard,K.D., Newcombe,D., Golebiewski,L., Petrosino,J.F., Nicholson,W.L., Fox,G.E., Venkateswaran,K., Highlander,S.K. and Weinstock,G.M. (2007) PLoS ONE 2 (9), E928.