ISAme2

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_009633 ND Alkaliphilus metalliredigens
Alkaliphilus metalliredigens QYMF
DNA section
IS Length : 1838 bp

Ends


IR Length : 10

IRL : GGGACGGTTCGGGATCAGTGAAAAAGTCTCTTATAAAGAGTTAATAATAT
IRR : GGGACGGTTCTTTCGATTAAGTGATGGAGATAAGAGTCTGTACAATACCT

Insertion site


Left flankDirect repeatRight flankDR Length
GAAATCCAATCAAGTGTGATTTCT0

DNA sequence

GGGACGGTTCGGGATCAGTGAAAAAGTCTCTTATAAAGAGTTAATAATATGATACTAGCTTTTGCTTGAAAACGTATAAAAACTAGTGAAAGTATTGAAT
TCACTAGTTTTGATCAGATGAAAACCCCCATATATGGTATAATATAAGTACGACCAAATACAACCAATAGGGGGTAATATGCTTCGACTAAAGCCAAAGC
AAATATCTTTTCACTCATCATTATATAATAAAATACCAGAAAATCATATACTAAAAAGAATAGATTCAGTAGTAGATTTTAGCTTTATCAATGGTTTACT
AGAGAATAGCTATTGCAAAAAATTTGGACGACCTGCGAAAGAACCGGAATTGATGTGCAAGTTACTTTTTTTGCAGCATTTATATAATCTATCAGATGAA
AAGGTCAAAGAAGAAGCGAGTTTAAACCTAGCATATATGTACTTTATTGGTATTAATCCAGAAGATTCTCTTCCGGATAAAAGTCTTTTATCTAAATTCA
GAACACAACGACTTGGACAGTCAACGCTAGATGAAATCACCACAGAAATCGTAAGACAATGTGTAGTAAAAGGAATCGTTGAGGGAAAGAGTGTAAGTAT
TGATGCTACCCATATAGAGGCGAATACAATAAAGAAAACTCCAGAACGATTAATGAAGCATTTAGGAAGAAAAATCATCAAGACATATGGAGAAGAAACA
TCCCAAGATCTAGAAGGTATACCTGAGGTGCCAGACTATAAAGAAATTACAGATCATAAAGAGGCAAAAGCTGCAATGAAAGAATACCTAGAAATTGTAA
TTGATCAAGCAGAGAGAAATATATCTACTAACGAGCAAAAAACAGCAGAAGTAATTAAACAGGCTAAAGATATATTAAATGATCCCAAGTTTATCAATCA
AAAAGGGATACGCTCATTAATAGATGTAGATGCAAGAGTTGGACGTAAAAGTAAAACGCAAGATTTTTTTGTTTAAAACGGAATTTATTATGACAACAGA
TGAACGAATTATCACCTCGATAAATACAGCCGATGGAGCGTATCCAGATGGTAACCATGTAAAAGAAATGCTGGACCAAACAAAGAAAACCGGCATAGCT
GTTGAAGAAGTTTATGGAGATAAAGCATATTTTAGAAAATCAATATTAGATGATATAAAAGAAATGAAAGCAAAAGCTTTCATACCAGTTAGTACATCCG
CTTACAGAATTGATGAAAGTGAGTATACATATAACAAAGACTCAGATGAATGGCAGTGTAGTCAAGGAAATACAACCATAAATAAAAAGCATTATAAATA
TAAAAGTAAAGCAAGAGAAAGAGCAGGTTATAAATACTATTTTGAAATGAGTCAATGCAAAGCATGTCCTAAACATGATGAATGTGCTAAAAAAAGCGCC
AGAAAAATACTTATAGTAGGTTTAAATGCTTCAGAATTTTATGAAATATCGCAGTATCAGAAAACAGATGAGTTCAAGGAACAATATAAAAAGAGAGCTT
CTATTGAAGGTAAAAATGCCGAACTCAAAAGATTTCATGGGCTACATCGAGCAAGAGGATATGGTTTAATAAGTGTGTCAAAACAATCAAAGCTAGCTGC
AATAGCGGTAAATATTAAGAGGATAGCAGCAATAATCTCCTCTTTTTTATCTAATTTTAAGGGTATTTTTGAAATGACAGACTATTTTTCGGATTTGTCG
AAATTTCTTGCGATTTAACGAAAAAAAGGACCAGTTTTTCACTGGTCCCGGATGGTTCTGTTGATTAATCGTTAAAATTTAAGATTGTAGGTATTGTACA
GACTCTTATCTCCATCACTTAATCGAAAGAACCGTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1540 bp513 aa1791718+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLRLKPKQISFHSSLYNKIPENHILKRIDSVVDFSFINGLLENSYCKKFGRPAKEPELMCKLLFLQHLYNLSDEKVKEEASLNLAYMYFIGINPEDSLPD
KSLLSKFRTQRLGQSTLDEITTEIVRQCVVKGIVEGKSVSIDATHIEANTIKKTPERLMKHLGRKIIKTYGEETSQDLEGIPEVPDYKEITDHKEAKAAM
KEYLEIVIDQAERNISTNEQKTAEVIKQAKDILNDPKFINQKGIRSLIDVDARVGRKSKTQDFFLFKTEFIMTTDERIITSINTADGAYPDGNHVKEMLD
QTKKTGIAVEEVYGDKAYFRKSILDDIKEMKAKAFIPVSTSAYRIDESEYTYNKDSDEWQCSQGNTTINKKHYKYKSKARERAGYKYYFEMSQCKACPKH
DECAKKSARKILIVGLNASEFYEISQYQKTDEFKEQYKKRASIEGKNAELKRFHGLHRARGYGLISVSKQSKLAAIAVNIKRIAAIISSFLSNFKGIFEM
TDYFSDLSKFLAI

 

Blast result :
Comments
ISAme2 is 44% aa similar to ISBfun3.
References
1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Ye,Q., Zhou,J., Fields,M. and Richardson,P. (2007) Direct submission GenBank.