ISfinder Mirror Site

ISMae25

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010296	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1246 bp

Ends

IR Length : 26/30

IRL : TGGACTGTTTCAGCTAAAATAGGGCAATAGCTCGAACAAGCAGAGGACAT
IRR : TGGACTGTTTCAGCTAATTTGGTGCAATAGATTCTCCCGATTCCTCCTCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACAATTATTT	GTAG	GATGGGTTAG	4
CTAACGCACC	CTAC	CTACTCATTT	4
TTAACGCACC	CTAC	GAAAGCTTAT	4
CTAACCCATC	CTAC	AAATAATTGT	4
TCAAACCAAT	GTAG	GGGGAAATAA	4

DNA sequence

TGGACTGTTTCAGCTAAAATAGGGCAATAGCTCGAACAAGCAGAGGACATCAATCATGGGAAGAGGTCGGCGAGATAAAGTCAATCTAACAGGGGAACAA
AGAGAAAACCTCGAACAAATCAGTCGTAATGGCTATGCACCAGCTAAAAAAATTCTCCACGCTCGGATTTTGCTGATGTGTGACGAGGGAGAACAGGCGA
AAAGGAAATGGACAGATGAAGAAATAGGCGAAGCTTTAGAAGTTCATAGAAATACAGTGGGACGTATTCGTCAAAGATTTCTTCAAAAAGGCGAAAAACC
AGCATTAGAACGGAAATCGAGAAAAACTCCCCCCACTCCGGCAAAAGTTGATGGAGCCGCCGCCGCCCAAATCATTGCCCTGTGCTGTTCGGAGCCACCA
TCTGGCCGAGCCGAGTGGACAATCCGACTATTAACCTCGGAACTCAAACAAAGACAAATTATCACCGAGATTTCCAGTCCAACGGTGTGGCGTACTCTAA
AAAAAACCAATTACGCCCTTGGAAAACCCAAAGATACTGTATTCCGGAACAGGATTTAGCCCGATTTGTTGCCCAGATGGAAGTTGTCCTTGACCTGTAT
GGCACTCAGCCATCGGAAGAAGAACCGTTAATCGCGATGGATGAAGCATCAAAGCAACTACTTGGAGAAGTTTACCCTCCGATACCCATGCAACCTGGAC
AAGATAAAAAAGAAGACTATCACTATAGTCGTGAAGGGGTTCAAGCCTTGTTCATGTTTTTTGACCCCCATCGAGGATGGAGACGGGTGAGTAACCGAGA
TAGTCGAACCCGAATAGATTGGGCAGAAGAAATTCGTCAATTATTGGATGTGGACTATCCAAAGGCTCGAAAAGTCAAGCTCGTCTGTGATAATCTCAAC
ACTCATAACATAGCCTCGCTTTATGAAGCATTTCCAGCACCCGTTGCTCATCGTTTAGCTAGAAGACTGGAAATTTATTACACACCTCGTAATGGTAGCT
GGTTAAATGTAGCCGAAACTGAACTAAGCGTCTTATCGAGGCAATGTTTAGATAGAAGGATTTCTAGCAAGGAAGAACTGAAAAGGGAGATAGAAACCTG
GCAAAAAGAACGTAATCAGACTGCATCTACAGTAATATGGACGTTTACGACCAGCGATGCTAGGGTCAAGCTGAAACATCTTTATCCTGTGTTTGAGGAG
GAGGAATCGGGAGAATCTATTGCACCAAATTAGCTGAAACAGTCCA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
504 bp	167 aa	56	559	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MGRGRRDKVNLTGEQRENLEQISRNGYAPAKKILHARILLMCDEGEQAKRKWTDEEIGEALEVHRNTVGRIRQRFLQKGEKPALERKSRKTPPTPAKVDG
AAAAQIIALCCSEPPSGRAEWTIRLLTSELKQRQIITEISSPTVWRTLKKTNYALGKPKDTVFRNRI

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
882 bp	293 aa	352	1233	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

WSRRRPNHCPVLFGATIWPSRVDNPTINLGTQTKTNYHRDFQSNGVAYSKKNQLRPWKTQRYCIPEQDLARFVAQMEVVLDLYGTQPSEEEPLIAMDEAS
KQLLGEVYPPIPMQPGQDKKEDYHYSREGVQALFMFFDPHRGWRRVSNRDSRTRIDWAEEIRQLLDVDYPKARKVKLVCDNLNTHNIASLYEAFPAPVAH
RLARRLEIYYTPRNGSWLNVAETELSVLSRQCLDRRISSKEELKREIETWQKERNQTASTVIWTFTTSDARVKLKHLYPVFEEEESGESIAPN

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1178 bp	392 aa	56	1233	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGRGRRDKVNLTGEQRENLEQISRNGYAPAKKILHARILLMCDEGEQAKRKWTDEEIGEALEVHRNTVGRIRQRFLQKGEKPALERKSRKTPPTPAKVDG
AAAAQIIALCCSEPPSGRAEWTIRLLTSELKQRQIITEISSPTVWRTLKKNQLRPWKTQRYCIPEQDLARFVAQMEVVLDLYGTQPSEEEPLIAMDEASK
QLLGEVYPPIPMQPGQDKKEDYHYSREGVQALFMFFDPHRGWRRVSNRDSRTRIDWAEEIRQLLDVDYPKARKVKLVCDNLNTHNIASLYEAFPAPVAHR
LARRLEIYYTPRNGSWLNVAETELSVLSRQCLDRRISSKEELKREIETWQKERNQTASTVIWTFTTSDARVKLKHLYPVFEEEESGESIAPN

Blast result :

Comments

ISMae25 is 60% aa similar to ISArch4.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.

References

1] Modified and submitted by ISfinder (2010)

2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.