ISAcfe2
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013124 | ND | Acidimicrobium ferrooxidans | Acidimicrobium ferrooxidans DSM 10331 |
DNA section
IS Length : 1771 bp
Ends
IR Length : 17/23
IRL : GAGTCCCCTGAACAACTCCTCCACGTAGGGCGGCTACGGGAAGCGCTTCC
IRR : GAGCCCTCTGAACAAGCCCCTCCCTGGTGGTGGCTGAGCACGCCCACCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGTGGTCCG | CTAG | CGCGACCCGC | 4 |
| AGTGCGACTG | CTAG | ACGCGCTCAG | 4 |
| CAGCAGCGGG | CTAG | GGCCGGTCGA | 4 |
| GGTTGACGTT | CTAG | CTGTGGACTT | 4 |
| GCACTCCATG | CTAG | GCACACGCAC | 4 |
| TCGCTCGGCC | CTAG | GTGTCCGCGG | 4 |
| GTGAGCGTGC | CTAG | AGGCCGACGA | 4 |
DNA sequence
GAGTCCCCTGAACAACTCCTCCACGTAGGGCGGCTACGGGAAGCGCTTCCCTCCGCCTGTCAAGCGCCATTTGCCCAGCTCAGCTAGACTTTTTGCATGC
TGGGCACCGAATCAGACCAGGCACACCTCACCGCGACGGAGGCCTTCTGCTCCTCGCTCATCGAGCCAGGGTCCATCTACGCCTTCCTCGCCGAGCACCG
CAGAGAGCTCTTCCCCGACGAGCGCTTCCGCCATCTCTACCCCTCCACGACCGGCAGACCCTCCATCCCCGCCTCGAGGGTGCTCGCGGTCATGGTGCTC
CAGGTGCTCGAGGGGCTCTCGGACACCGAGGCGACAGAGCAGGTCCGCTACAACCTCCGCTGGAAGTACGCCCTTGGTCTTGATCTCGAGGACCCAGGCT
TTCACCCCACGGTGCTCACCTACTGGCGCAGGCGCATCGCCACCTCCGAGACCCCAAGACTCATCGGCGAGCTCGTGGCCGAGGTGATCGGTGCCACGGG
GGTGCTCAAGGGCAAGACGAAGCGCGTCGTCGACTCCACCGTGCTCGCCGATGCGGTTGCCACCCAAGACACCATGACCCAACTTGTCGCCCAGATCAAC
CGGGTACGCAGGCTGATCCCCGAACTACGCGACATTCCTCTGTCACCAGCGATCGACTACACCCGCGCAAAGCCTGCCATCGACTATCGGGACGAAGAGG
CAGTGGTGCGCACGGTGAGTGCCCTCGTCGCCGATGCCACTGCCCACCTCGCCCATGCAGAGAAGCTCGCATCCCTCACCGAGCCACAGACCGAAGCCCT
TGGTCTCCTTGGGCTCGTGGCGGGCCAAGACGTGGAGTGTGTCGATGCCAAGGAAGGTCGCTGGCGCATCGCGAGGCGGGTAGCCACAGACAGGGTCATC
TCCACCGTGGACCCTGACGCTCGCCACGTCCACAAGTCCCGTGCCCGTGCCATCGATGGCTACAAGGGCCACGTGGCCGTCGAGCCAGACAGTGGCATCG
TCACCGCTGCGACCATCACGAAGGGCACCGTGCCGGACGCAGCAGTGGCGAGCGAGCTCCTTCGTGACGAGGACGGCCCGCGCACGGTCTATGGCGACAG
CGCCTATGCCACGAGCGAGGTCTCCAACGAGCTCGCCGCTCGTGGCCACGACGAGGTGATCAAGCCTCGGCCCCTTGCCATGGCGGTCCCTGGTGGCTTT
ACCATCGATGACTTCGTCGTCGAGGAGGGATGGGTCAGCTGTCCCCAAGGACACCGAGTACCCATCTCCGCCAAGGGTCGTGCTTCCTTTGTCAAGCACT
GCACCGGCTGTCCGCTCCGGGATCGCTGCACGCGCTCGAAGCGAGGACGGGTCCTCACCTTCACCCCTGCCACCTGGCACGCAATCAGCCAGCGGGCGCA
CTTTGGGGACCCTGCCGTTCGTCTCGACTATCAGCGCACCCGACCGAACGTCGAGCGGATCCACGCACAGCTCAAACGCAAGCTCTCCGGCGCGAGGCTG
CGCTACCTGGGGCTCGTGCGCAACCGACTCCACTTCGAGCTGCTCTGTGCCACCTGGAACCTGAAGGTGCTCCTTCGCCTGGGGCTCACCCGGGTGGGTG
GTGGCTGGGTGCTTGCCACCTGAGAGGCGCTCGGGTCCTCTCCTCCTCGCCCCACAGGAGGGGCGAGGAGGAGAGGGTGAGGAGCGAGATGGGAACGGGT
CAGCTGGGCTCGTTCCCGGGCTGGGTGGGCGTGCTCAGCCACCACCAGGGAGGGGCTTGTTCAGAGGGCTC
TGGGCACCGAATCAGACCAGGCACACCTCACCGCGACGGAGGCCTTCTGCTCCTCGCTCATCGAGCCAGGGTCCATCTACGCCTTCCTCGCCGAGCACCG
CAGAGAGCTCTTCCCCGACGAGCGCTTCCGCCATCTCTACCCCTCCACGACCGGCAGACCCTCCATCCCCGCCTCGAGGGTGCTCGCGGTCATGGTGCTC
CAGGTGCTCGAGGGGCTCTCGGACACCGAGGCGACAGAGCAGGTCCGCTACAACCTCCGCTGGAAGTACGCCCTTGGTCTTGATCTCGAGGACCCAGGCT
TTCACCCCACGGTGCTCACCTACTGGCGCAGGCGCATCGCCACCTCCGAGACCCCAAGACTCATCGGCGAGCTCGTGGCCGAGGTGATCGGTGCCACGGG
GGTGCTCAAGGGCAAGACGAAGCGCGTCGTCGACTCCACCGTGCTCGCCGATGCGGTTGCCACCCAAGACACCATGACCCAACTTGTCGCCCAGATCAAC
CGGGTACGCAGGCTGATCCCCGAACTACGCGACATTCCTCTGTCACCAGCGATCGACTACACCCGCGCAAAGCCTGCCATCGACTATCGGGACGAAGAGG
CAGTGGTGCGCACGGTGAGTGCCCTCGTCGCCGATGCCACTGCCCACCTCGCCCATGCAGAGAAGCTCGCATCCCTCACCGAGCCACAGACCGAAGCCCT
TGGTCTCCTTGGGCTCGTGGCGGGCCAAGACGTGGAGTGTGTCGATGCCAAGGAAGGTCGCTGGCGCATCGCGAGGCGGGTAGCCACAGACAGGGTCATC
TCCACCGTGGACCCTGACGCTCGCCACGTCCACAAGTCCCGTGCCCGTGCCATCGATGGCTACAAGGGCCACGTGGCCGTCGAGCCAGACAGTGGCATCG
TCACCGCTGCGACCATCACGAAGGGCACCGTGCCGGACGCAGCAGTGGCGAGCGAGCTCCTTCGTGACGAGGACGGCCCGCGCACGGTCTATGGCGACAG
CGCCTATGCCACGAGCGAGGTCTCCAACGAGCTCGCCGCTCGTGGCCACGACGAGGTGATCAAGCCTCGGCCCCTTGCCATGGCGGTCCCTGGTGGCTTT
ACCATCGATGACTTCGTCGTCGAGGAGGGATGGGTCAGCTGTCCCCAAGGACACCGAGTACCCATCTCCGCCAAGGGTCGTGCTTCCTTTGTCAAGCACT
GCACCGGCTGTCCGCTCCGGGATCGCTGCACGCGCTCGAAGCGAGGACGGGTCCTCACCTTCACCCCTGCCACCTGGCACGCAATCAGCCAGCGGGCGCA
CTTTGGGGACCCTGCCGTTCGTCTCGACTATCAGCGCACCCGACCGAACGTCGAGCGGATCCACGCACAGCTCAAACGCAAGCTCTCCGGCGCGAGGCTG
CGCTACCTGGGGCTCGTGCGCAACCGACTCCACTTCGAGCTGCTCTGTGCCACCTGGAACCTGAAGGTGCTCCTTCGCCTGGGGCTCACCCGGGTGGGTG
GTGGCTGGGTGCTTGCCACCTGAGAGGCGCTCGGGTCCTCTCCTCCTCGCCCCACAGGAGGGGCGAGGAGGAGAGGGTGAGGAGCGAGATGGGAACGGGT
CAGCTGGGCTCGTTCCCGGGCTGGGTGGGCGTGCTCAGCCACCACCAGGGAGGGGCTTGTTCAGAGGGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1527 bp | 508 aa | 97 | 1623 | + | No |
Chemistry : DDE
ORF sequence :
MLGTESDQAHLTATEAFCSSLIEPGSIYAFLAEHRRELFPDERFRHLYPSTTGRPSIPASRVLAVMVLQVLEGLSDTEATEQVRYNLRWKYALGLDLEDP
GFHPTVLTYWRRRIATSETPRLIGELVAEVIGATGVLKGKTKRVVDSTVLADAVATQDTMTQLVAQINRVRRLIPELRDIPLSPAIDYTRAKPAIDYRDE
EAVVRTVSALVADATAHLAHAEKLASLTEPQTEALGLLGLVAGQDVECVDAKEGRWRIARRVATDRVISTVDPDARHVHKSRARAIDGYKGHVAVEPDSG
IVTAATITKGTVPDAAVASELLRDEDGPRTVYGDSAYATSEVSNELAARGHDEVIKPRPLAMAVPGGFTIDDFVVEEGWVSCPQGHRVPISAKGRASFVK
HCTGCPLRDRCTRSKRGRVLTFTPATWHAISQRAHFGDPAVRLDYQRTRPNVERIHAQLKRKLSGARLRYLGLVRNRLHFELLCATWNLKVLLRLGLTRV
GGGWVLAT
GFHPTVLTYWRRRIATSETPRLIGELVAEVIGATGVLKGKTKRVVDSTVLADAVATQDTMTQLVAQINRVRRLIPELRDIPLSPAIDYTRAKPAIDYRDE
EAVVRTVSALVADATAHLAHAEKLASLTEPQTEALGLLGLVAGQDVECVDAKEGRWRIARRVATDRVISTVDPDARHVHKSRARAIDGYKGHVAVEPDSG
IVTAATITKGTVPDAAVASELLRDEDGPRTVYGDSAYATSEVSNELAARGHDEVIKPRPLAMAVPGGFTIDDFVVEEGWVSCPQGHRVPISAKGRASFVK
HCTGCPLRDRCTRSKRGRVLTFTPATWHAISQRAHFGDPAVRLDYQRTRPNVERIHAQLKRKLSGARLRYLGLVRNRLHFELLCATWNLKVLLRLGLTRV
GGGWVLAT
Blast result :
Comments
ISAcfe2 is 95% aa similar to ISAcfe1.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Clum,A., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Lang,E., Spring,S., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Clum,A., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Lang,E., Spring,S., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.