ISfinder Mirror Site

ISMae24

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010296	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1084 bp

Ends

IR Length : 19/23

IRL : AGTAGTCATGCAAAATTAATTACCCAAATAAAAAATTAAGAAGTATAATA
IRR : AGTACCTAGGCAAAATTAATTACATACTCGCCTCCAAAATTGTCCAGCAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATAACCAGC	TA	CTTAAATCCC	2
AAAGAATGAT	TA	TTTATTCCCC	2
GAAGACCTGT	TA	TAGCTGGAAC	2
CTTACTCAAA	TA	TCAGTCTAGC	2
GCTCTTCTCC	TA	ATTGCTTTTC	2

DNA sequence

AGTAGTCATGCAAAATTAATTACCCAAATAAAAAATTAAGAAGTATAATACATAAAAAAACGAAGCAGCAGGAAAATACAATGAGATTGATTAGAGACCT
AAACCCCGAGAGCCAGAAAATGCTAGAGAGAATTTATCGAGCTAGTAAACATCATCAAGTAAGAGAGCGAGCGAAATGTATACTCTTAAGTTTTCAGGGA
ACCACGATAGAAGAATTGAGCGGAATATTTGGAGTTACGAGAAAGACCATCTATAATTGGTTGACGGCCTGGGAAGATAGAAAACTAATTGGTTTTTATA
ATCGTCGAGGAAGAGGGAGAAAACCTAAATTGACAGAAGCCCAAGGTCAACAAGTTATTGACTGGGTAAAAGAAGAACCGAAAAGCTTAAAAAAAATCCA
GATAAAAATTGTAGAAGAAGGGAAATTAACCGTAAGCAAAGACACGATAAAAAGACTCATAAAAAAAATCAACATGAGGTGGAAAAGGGTGAGAAGAGGG
GTCGCCAAAACCCCTGATGAGTGGGAGCTTGAGGTCAAACTACCTATTTTAGAAGAACTAAAAAAACAGGAAAAAAGAGGAGAGATTGAGATAGGATATT
TGGATGAAATGGGAGGGGATTCAAAGCCTTGTATTCCTGACGCTTGGCAAGAAGAAAAAACCACGATAAAGTTACCACCAATTGAAGGTAAAAGACTAAA
TATTTTAGGAATAATGAAACGAGATAATCAATTATTTTATGAGACACAGGTCGGAACGGTTACTAGCGAGATAGTTATTAATTTTCTGGATAAATATTGC
CAAAATATACAGAAAAAAACTGTCATAATAATTGACCAAGCTTCCATTCATACCAGCGAGGCATTTATGGAGAAACTTGAGGAATGGGAAAAGAAAAACT
TGAAAATATTTTGGTTGCCCACTTATTCACCTCATTTAAATTTAATTGAAATATTATGGAGATTTTTAAAATATGAATGGATTGAATTTAGCGCCTATAA
AGACCGAAAGAGCCTCCTCGCTTACGTTAAAAAAGTGCTGGACAATTTTGGAGGCGAGTATGTAATTAATTTTGCCTAGGTACT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
999 bp	332 aa	81	1079	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRLIRDLNPESQKMLERIYRASKHHQVRERAKCILLSFQGTTIEELSGIFGVTRKTIYNWLTAWEDRKLIGFYNRRGRGRKPKLTEAQGQQVIDWVKEEP
KSLKKIQIKIVEEGKLTVSKDTIKRLIKKINMRWKRVRRGVAKTPDEWELEVKLPILEELKKQEKRGEIEIGYLDEMGGDSKPCIPDAWQEEKTTIKLPP
IEGKRLNILGIMKRDNQLFYETQVGTVTSEIVINFLDKYCQNIQKKTVIIIDQASIHTSEAFMEKLEEWEKKNLKIFWLPTYSPHLNLIEILWRFLKYEW
IEFSAYKDRKSLLAYVKKVLDNFGGEYVINFA

Blast result :

Comments

ISMae24 is 67% aa similar to ISAcma15.

References

1] Modified and submitted by ISfinder (2010)

2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.