ISAcfe1

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013124 ND Acidimicrobium ferrooxidans
Acidimicrobium ferrooxidans DSM 10331
DNA section
IS Length : 1772 bp

Ends


IR Length : 14/15

IRL : GAGCCTCCTGAACAACTCCTCCACGTAGCGCGGCGACGCACGGCGCTCCT
IRR : GAGCCTTCTGAACAAGCCCCTCCCTGGTAGCGACTGATGCCAGCCACCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CCGTAGGCCTCTAGGACTCGCTCC4

DNA sequence

GAGCCTCCTGAACAACTCCTCCACGTAGCGCGGCGACGCACGGCGCTCCTCCCCTCCTGCCCAGCGCCATTTGCCCAGCTCAGCTAGACTTTTTGCATGT
TGGGCACCGAATCAGACCAGGCACACCTCACCGCGACGGAGGCCTTCTGCTCCTCGCTCATCGAGCCAGGGTCCATCTACGCCTTCCTCGCCGAGCACCG
CAGGGAGCTCTTCCCCGACGAGCGCTTCCGCCACCTCTATCCCTCCACGACCGGCAGACCCTCCATCCCCGCCTCGAGGGTGCTCGCGGTCATGGTGCTC
CAGGTGCTCGAGGGGCTCTCGGACACCGAGGCGACAGAGCAGGTCCGCTACAACCTGCGCTGGAAGTACGCCCTCGGTCTCGACCTCGAGGACCCAGGTT
TTCACCCCACGGTGCTCACCTACTGGCGCAGGCGCATCGCCACCTCCGAGACCCCAAGACTCATCACCGAGCTCGTGGCCGAGGTGATCGGTGCCACGGG
GGTGCTCAAGGGCACGACGAAGCGCGTCGTCGACTCCACCGTGCTCGCCGATGCGGTCGCGACCCAAGACACCATGACCCAACTCGTCGCTCAGATCAAC
CGGGTACGCAGGCTGATCCCCGAGCTCCGCGAAGTTCCTCTGTCACCAGCGATCGACTACACCCGTCCAAAGCCTGCCATCGACTACCGAGACGAAGAAG
CAGTGGTGCGCACGGTGAGCGCCCTCGTCGCCGATGCCACCGCCCACCTCGCCGAGGCCGAGAAGCTCGCGTCTCTCACCGAGCCCCAGCGCGAGGCCCT
GGGCCTCCTTGGGCTCGTGGCGGGCCAGGACGTCGAGTGCACAGATGCCACCGCAGGCCGCTGGCGCATCGCGAGGCGAGTGGCCCGCGACAGGGTCATC
TCCACCGTGGACCCCGACGCTCGCCATGTGCACAAGTCCCGTGCCCGTGCCATCGATGGCTACAAGGGCCACGTGGCCGTCGAGCCAGACAGCGGCATCG
TCACCGCCTCGACCATCACGACGGGTAGCGTGCCTGATGCACAGGTCGTGCCCGAGCTCCTTGGTGACGAGGACGGCCCACGCACGGTCTATGGCGACAG
CGCCTACGCCACGAGCGAGGTCTTCAACGAGCTTGCCGCTCATGGCCACGACGAGGTGATCAAGCCTCGGCCCCTTTCTATGGCGGTCCCTGGTGGCTTC
ACGATCGACGACTTCGTCGTCGAGGAGGGATGGGTTAGCTGTCCTGCGGGGCACCGAGTCCCCATTTCCGCGAAGGGCCGTGCCTCCTTCGCCAAGCACT
GCGATGGCTGTCCGCTCCGGGAGCGCTGCACACGATCGAAGCGCGGACGGGTCCTCACCTTCACCCCTGCCACCTGGCACGGCATCAGCCAGCGGGCGCA
CTTTGGGGACCCGGCTGTCCGTCTCGACTACCAGCGCACCCGACCGAACGTCGAGCGGATCCACGCACAGCTCAAGCGCAAGCTCTCCGGGGCGAGGCTG
CGCTACCGGGGACTCGTGCGCAACCGCCTGCACTTCGAGCTGCTCTGTGCGACCTGGAACCTGAAGGTGCTGCTACGCCTGGGGCTCACCCGAGTGGGTG
GTGGCTGGGTGCTTGCCACCTGAGAGACGCTCGGGCCCTCTCCTCCGCACCCACGAGGAGGGGACGAAGAGGAGAGGGTGAGGAGCTGGGCGAGCACGGG
TCAGCTGAGCTCGTTCCCGGGCTGGGTGGCTGGCATCAGTCGCTACCAGGGAGGGGCTTGTTCAGAAGGCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1527 bp508 aa971623+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLGTESDQAHLTATEAFCSSLIEPGSIYAFLAEHRRELFPDERFRHLYPSTTGRPSIPASRVLAVMVLQVLEGLSDTEATEQVRYNLRWKYALGLDLEDP
GFHPTVLTYWRRRIATSETPRLITELVAEVIGATGVLKGTTKRVVDSTVLADAVATQDTMTQLVAQINRVRRLIPELREVPLSPAIDYTRPKPAIDYRDE
EAVVRTVSALVADATAHLAEAEKLASLTEPQREALGLLGLVAGQDVECTDATAGRWRIARRVARDRVISTVDPDARHVHKSRARAIDGYKGHVAVEPDSG
IVTASTITTGSVPDAQVVPELLGDEDGPRTVYGDSAYATSEVFNELAAHGHDEVIKPRPLSMAVPGGFTIDDFVVEEGWVSCPAGHRVPISAKGRASFAK
HCDGCPLRERCTRSKRGRVLTFTPATWHGISQRAHFGDPAVRLDYQRTRPNVERIHAQLKRKLSGARLRYRGLVRNRLHFELLCATWNLKVLLRLGLTRV
GGGWVLAT

 

Blast result :
Comments
ISAcfe1 is 58% aa similar to ISMgi3.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Clum,A., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Lang,E., Spring,S., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.