ISAmi1

  • Family IS5
  • Group ISL2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_013093 ND Actinosynnema mirum
Actinosynnema mirum DSM 43827
DNA section
IS Length : 883 bp

Ends


IR Length : 17

IRL : AGGGATGTCTCGTAACTCGGTGATCGGTCGCCATGGCCGATCGGCGATCA
IRR : AGGGATGTCTCGTAACTGATCTCGTGAGATGCCCCGGCGGTGCCGGTCAG

Insertion site


Left flankDirect repeatRight flankDR Length
GTGACGCCTAACCAACCAGA0

DNA sequence

AGGGATGTCTCGTAACTCGGTGATCGGTCGCCATGGCCGATCGGCGATCATGGTCGGATGCAGGTCATATCAGCATCCCGATCGGAGTGGATCGTCCCGT
TCACGGGCTTGCAACCAGGCCAGTTCCGTAGATTGGTCCGTGTCGTGGCCGAACGCGGCGGTGACACGATCGCCGACGGTCGCCCCGGTCGGCAATGGCG
GCTCGACCTGGCCGACCGGGTACTGCTGGTCGCGACCTACTGGCGGACCAACCTCACGATGCGCCAGATCGGACCACTGTTCGGGGTATCGCACTCCGCA
GCGCATCGGGTCGTGGACAGCCTGGGCCCGCTGCTGGCCCTGGCGCCGGTGCGGCGCCGCCGGATCGACCAGATCGCCATCGTCGACGGAACCCTCGTGC
CCACTCGTGATCACCGCCTGGCGGCGAGGTCGAAGAACTACCGGTACTCCACCAACCTGCAGGTCGCCATCGACGCCGACACCCGCCTGGTCATCGACAT
CGGCGACCCGCGACCGGGCAACCGCAACGACTGCACCGTCTACCGCGACTCTGGCATCAAGGACACCCTCGCCGGACGACCGGTGATGGCCGACGGCGGC
TACCAGGGCAACACCGGCGTGATCATGCCCTACCGCAAACCCCGCGACGGCAGCGAACTTGCCGACTGGCAGGAAGACCTCAACACCACCCACCGCGCGA
TCCGCGCCCGCGTCGAACACACCTTGGCCCGGATGAAGAACTGGAAGATCCTGCGCGACCATCGCCGCGCCGCCCACACCCTGCACGACACCGCTCTGGG
CATCGCCCATCTTCACAACCTCGCCATCACCGGCTGACCGGCACCGCCGGGGCATCTCACGAGATCAGTTACGAGACATCCCT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
780 bp259 aa58837+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQVISASRSEWIVPFTGLQPGQFRRLVRVVAERGGDTIADGRPGRQWRLDLADRVLLVATYWRTNLTMRQIGPLFGVSHSAAHRVVDSLGPLLALAPVRR
RRIDQIAIVDGTLVPTRDHRLAARSKNYRYSTNLQVAIDADTRLVIDIGDPRPGNRNDCTVYRDSGIKDTLAGRPVMADGGYQGNTGVIMPYRKPRDGSE
LADWQEDLNTTHRAIRARVEHTLARMKNWKILRDHRRAAHTLHDTALGIAHLHNLAITG

 

Blast result :
Comments
ISAmi1 is 70% aa similar to IS112.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank