ISCaa5
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010830 | ND | Candidatus Amoebophilus | Candidatus Amoebophilus asiaticus 5a2 |
DNA section
IS Length : 920 bp
Ends
IR Length : 21/25
IRL : ACCTAAGTTGCTAGTAATAGATAAGGCAAAGAGTAAAAAACCTCCGATCT
IRR : ACCTAAGTTGCTAGTTAAGAAAAGCTTTGTTGATTTGAAAAGCTAGCAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATATAGTTC | TATATA | TACAAAACTA | 6 |
| TCTATTTTTA | TATATA | AGTTAAGAAA | 6 |
| TAAATACCAC | TAGTTA | GCAAAATTCT | 6 |
| AAGTTTTATC | TATATA | TTTGTCAAAA | 6 |
| TTATACCTCA | TAAa/tTA | AGTAACCAAG | 8 |
| ATTTCTTGAG | TAa/tTTA | ATGCAGGGTT | 8 |
| TTGTTTTGTA | TATa/tTA | CATTAACTGT | 8 |
| GGTATTCTCT | TAa/tATA | TTCTAAAGTC | 8 |
| GATAAAGTAT | TAc/tTTA | AGTAAAATAT | 8 |
| CTTTTTTGCT | TAc/ta/tTA | AGGCAATATA | 10 |
DNA sequence
ACCTAAGTTGCTAGTAATAGATAAGGCAAAGAGTAAAAAACCTCCGATCTTGTTTTTGTCTTAAACCAATAAATCGGAGGTCATTATGATTGAAAAATCA
ATAGCAATATACTCATTTATTGATACTTTACTCAAGTATTTACATCATCAAGAAGATAAAAAAAGGAAGTTGTCGGATGCAGAAGTACTCACAACAGCTA
TCATCTCAGCCTTATACTTTGGCGGACATCTTGACAAAGCTCGATCGTTTATGCATTCCACTAAGCTTATCCCTAACATGCTCGATAAAAGTAGGTACAA
TCGCCGCTTGCATGCTATAGGAGAAGAGATAACTTCGCTCTTTTTAGAAATAGGCACTTTAATCAAGCAAGTAGCCACTTGTAAGGACTTTGTATTGGAT
TCATTTCCTGTGCCTGTGTGCGATAACATACGTATTAGCAGATGTAAACTATTACAAAGTGAAGCTTACAGAGGCTACAAAGCCTCTATGCGCCGTTACT
TTTATGGCATTAAAGTGCAGCTTATTACTACTGATTGTGGTATTCCGGTCGAATTCTCAATAGTAGCTGGCAGCCAAGCGGATGTAAAAGGATTGCACCA
ACTGCCCTTTTCTATGCCTGCTGGTAGTGCACTTTATGCTGACTCGGCTTATACTAACTACCATCTAGAAGATATGTTGGCTGATGATAGGATTAAGCTT
TATTCTCAGCGTAAATCTAATGCTCATCGAAAGGATACGCCTTCTCTGGCTTATCTCAAAGAGCGCATGCGAAAAGTGATAGAGACCAGCATTAGTGGCA
TTAAAGGCCTTTTCTTAAGGAAGATTCATGCTGTTACCTTCCAAGGCTTTCTGATCAAAATACTCTTGTTCTTGCTAGCTTTTCAAATCAACAAAGCTTT
TCTTAACTAGCAACTTAGGT
ATAGCAATATACTCATTTATTGATACTTTACTCAAGTATTTACATCATCAAGAAGATAAAAAAAGGAAGTTGTCGGATGCAGAAGTACTCACAACAGCTA
TCATCTCAGCCTTATACTTTGGCGGACATCTTGACAAAGCTCGATCGTTTATGCATTCCACTAAGCTTATCCCTAACATGCTCGATAAAAGTAGGTACAA
TCGCCGCTTGCATGCTATAGGAGAAGAGATAACTTCGCTCTTTTTAGAAATAGGCACTTTAATCAAGCAAGTAGCCACTTGTAAGGACTTTGTATTGGAT
TCATTTCCTGTGCCTGTGTGCGATAACATACGTATTAGCAGATGTAAACTATTACAAAGTGAAGCTTACAGAGGCTACAAAGCCTCTATGCGCCGTTACT
TTTATGGCATTAAAGTGCAGCTTATTACTACTGATTGTGGTATTCCGGTCGAATTCTCAATAGTAGCTGGCAGCCAAGCGGATGTAAAAGGATTGCACCA
ACTGCCCTTTTCTATGCCTGCTGGTAGTGCACTTTATGCTGACTCGGCTTATACTAACTACCATCTAGAAGATATGTTGGCTGATGATAGGATTAAGCTT
TATTCTCAGCGTAAATCTAATGCTCATCGAAAGGATACGCCTTCTCTGGCTTATCTCAAAGAGCGCATGCGAAAAGTGATAGAGACCAGCATTAGTGGCA
TTAAAGGCCTTTTCTTAAGGAAGATTCATGCTGTTACCTTCCAAGGCTTTCTGATCAAAATACTCTTGTTCTTGCTAGCTTTTCAAATCAACAAAGCTTT
TCTTAACTAGCAACTTAGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 825 bp | 274 aa | 86 | 910 | + | No |
Chemistry : DDE
ORF sequence :
MIEKSIAIYSFIDTLLKYLHHQEDKKRKLSDAEVLTTAIISALYFGGHLDKARSFMHSTKLIPNMLDKSRYNRRLHAIGEEITSLFLEIGTLIKQVATCK
DFVLDSFPVPVCDNIRISRCKLLQSEAYRGYKASMRRYFYGIKVQLITTDCGIPVEFSIVAGSQADVKGLHQLPFSMPAGSALYADSAYTNYHLEDMLAD
DRIKLYSQRKSNAHRKDTPSLAYLKERMRKVIETSISGIKGLFLRKIHAVTFQGFLIKILLFLLAFQINKAFLN
DFVLDSFPVPVCDNIRISRCKLLQSEAYRGYKASMRRYFYGIKVQLITTDCGIPVEFSIVAGSQADVKGLHQLPFSMPAGSALYADSAYTNYHLEDMLAD
DRIKLYSQRKSNAHRKDTPSLAYLKERMRKVIETSISGIKGLFLRKIHAVTFQGFLIKILLFLLAFQINKAFLN
Blast result :
Comments
ISCaa5 is 54% aa similar to ISPasp3.
ISCaa5 is present in 10 copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
ISCaa5 is present in 10 copies in the Candidatus Amoebophilus asiaticus 5a2 genome.
References
1] Schmitz-Esser S, Tischler P, Arnold R, Montanaro J, Wagner M, Rattei T, Horn M. (2010) J Bacteriol., 192(4):1045-1057.