ISGau1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP009153 | ND | Gemmatimonas aurantiaca | Gemmatimonas aurantiaca T-27 |
DNA section
IS Length : 1276 bp
Ends
IR Length : 21/27
IRL : TGTCGCGTCCTGAATAACGTCTACACATCCTCTCACCTGTGGAGATGTGC
IRR : TGTCTAGTCCTAGAATTTGTCTACACGAGATGAGGGACTTGGTCGGACTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGCATGACC | GTATCTGTGC | 0 | |
| ACCACAAAAA | TCATGCTGTA | 0 | |
| ACTCTCTAAA | GTTACTCAAA | 0 |
DNA sequence
TGTCGCGTCCTGAATAACGTCTACACATCCTCTCACCTGTGGAGATGTGCTAACGTATGGAACGCAGCCGTCGCCAGTTTAGTCCCGAGTTGAAGCAACG
GATTGTCGAAGAGCTCGAAGCCGGGCACCTGTCGGTGCGGGAAGCGGCCCGGACCGTGCAGACCAGCGCGGCGATGGTGCATCACTGGCTGGAGGAGTTC
GGGCGGTATCGACCGAAACGGGATGTCGTGGAGGTGGTGATGAAGAGTGAACAGGAGCGCATTGCCGCGCTCGAGAAGGCGTTGGCCGAGGCGCATCTGA
CCCTCCAGGTCTACGACGAGCTCATCACCCAAGCGAACCGCGTGTACAAGACCGATCTAAAAAAAAGCTTTGGTACAACACCGCGCGGGCCTGTTGCGAC
GAGCGGGCCCTCCCCGTCCGGGCCGTCTGCGCGCGCCTCGGACGCACCCGCGACGCGTACTACAAACGTGCGGCCCGCGTCGGGGGCGGGGAGCGCGGGG
CGTCGAAAGCGGTAGTGCTCGCGCAGGTGGCCGTGGTGCGCGCGGCACAGCCCCGCGTCGGGACGCGGAAACTGCACACCCATTTGGCCGCCGCCGGCGT
GGCCGTCGGTCGTGATCGGCTCTTCACGTGGCTGCGGGCGGCGGCGGCGCTCGTGCCGCGCAAGCGGCGGCGGACGTTTACCACGTATTCGAAGCACGGG
TATGTCGTGGCGCCGAATCGCTTGAAGGACGCGGAGATCACGGGACCGCGTCAGGCGGTCGTGAGCGACAGTACGTATCTGCGCCTCGCGCCCGATCGCT
TCGCGTATTTGTTTCTCGTCACCGACGTGTATGCCCGCCGCATCGTCGGCTGGCATCTGAGCCAGGATCTCTCGCACCACAGTGCGCTGCAGGCGCTCCA
CCACGCGATCCAGACGATCGGCGCGGCCGATGGCGTGCTGCATCATTCGGACCGCGGGAGTCAGTACTGCTGTCACGCGTATCGCCGGGCTCTCGCCGCC
GCGCGCTTGCTACCCAGCATGACCGATGCGAATCACTGCTATCAGAACGCCATCGCCGAACGCGTCAATGGGATCTTGAAAGACGAATTTGATCTCGACG
CGATCTTCCCGTCTCTCCCAGCGGCTCAGCGGGCCGTCGCCGACGCCATTCACCGCTACAACACCGTCCGCCTCCACGGGAGTCTCCAGCTGCAGACCCC
CGCACACGTATTCACGCACGCAGCCTGAGTCCGACCAAGTCCCTCATCTCGTGTAGACAAATTCTAGGACTAGACA
GATTGTCGAAGAGCTCGAAGCCGGGCACCTGTCGGTGCGGGAAGCGGCCCGGACCGTGCAGACCAGCGCGGCGATGGTGCATCACTGGCTGGAGGAGTTC
GGGCGGTATCGACCGAAACGGGATGTCGTGGAGGTGGTGATGAAGAGTGAACAGGAGCGCATTGCCGCGCTCGAGAAGGCGTTGGCCGAGGCGCATCTGA
CCCTCCAGGTCTACGACGAGCTCATCACCCAAGCGAACCGCGTGTACAAGACCGATCTAAAAAAAAGCTTTGGTACAACACCGCGCGGGCCTGTTGCGAC
GAGCGGGCCCTCCCCGTCCGGGCCGTCTGCGCGCGCCTCGGACGCACCCGCGACGCGTACTACAAACGTGCGGCCCGCGTCGGGGGCGGGGAGCGCGGGG
CGTCGAAAGCGGTAGTGCTCGCGCAGGTGGCCGTGGTGCGCGCGGCACAGCCCCGCGTCGGGACGCGGAAACTGCACACCCATTTGGCCGCCGCCGGCGT
GGCCGTCGGTCGTGATCGGCTCTTCACGTGGCTGCGGGCGGCGGCGGCGCTCGTGCCGCGCAAGCGGCGGCGGACGTTTACCACGTATTCGAAGCACGGG
TATGTCGTGGCGCCGAATCGCTTGAAGGACGCGGAGATCACGGGACCGCGTCAGGCGGTCGTGAGCGACAGTACGTATCTGCGCCTCGCGCCCGATCGCT
TCGCGTATTTGTTTCTCGTCACCGACGTGTATGCCCGCCGCATCGTCGGCTGGCATCTGAGCCAGGATCTCTCGCACCACAGTGCGCTGCAGGCGCTCCA
CCACGCGATCCAGACGATCGGCGCGGCCGATGGCGTGCTGCATCATTCGGACCGCGGGAGTCAGTACTGCTGTCACGCGTATCGCCGGGCTCTCGCCGCC
GCGCGCTTGCTACCCAGCATGACCGATGCGAATCACTGCTATCAGAACGCCATCGCCGAACGCGTCAATGGGATCTTGAAAGACGAATTTGATCTCGACG
CGATCTTCCCGTCTCTCCCAGCGGCTCAGCGGGCCGTCGCCGACGCCATTCACCGCTACAACACCGTCCGCCTCCACGGGAGTCTCCAGCTGCAGACCCC
CGCACACGTATTCACGCACGCAGCCTGAGTCCGACCAAGTCCCTCATCTCGTGTAGACAAATTCTAGGACTAGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 459 bp | 152 aa | 57 | 515 | + | No |
Description : First part of the transposase
ORF sequence :
MERSRRQFSPELKQRIVEELEAGHLSVREAARTVQTSAAMVHHWLEEFGRYRPKRDVVEVVMKSEQERIAALEKALAEAHLTLQVYDELITQANRVYKTD
LKKSFGTTPRGPVATSGPSPSGPSARASDAPATRTTNVRPASGAGSAGRRKR
LKKSFGTTPRGPVATSGPSPSGPSARASDAPATRTTNVRPASGAGSAGRRKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 978 bp | 325 aa | 251 | 1228 | + | No |
Description : Second part of the transposase
ORF sequence :
TGAHCRAREGVGRGASDPPGLRRAHHPSEPRVQDRSKKKLWYNTARACCDERALPVRAVCARLGRTRDAYYKRAARVGGGERGASKAVVLAQVAVVRAAQ
PRVGTRKLHTHLAAAGVAVGRDRLFTWLRAAAALVPRKRRRTFTTYSKHGYVVAPNRLKDAEITGPRQAVVSDSTYLRLAPDRFAYLFLVTDVYARRIVG
WHLSQDLSHHSALQALHHAIQTIGAADGVLHHSDRGSQYCCHAYRRALAAARLLPSMTDANHCYQNAIAERVNGILKDEFDLDAIFPSLPAAQRAVADAI
HRYNTVRLHGSLQLQTPAHVFTHAA
PRVGTRKLHTHLAAAGVAVGRDRLFTWLRAAAALVPRKRRRTFTTYSKHGYVVAPNRLKDAEITGPRQAVVSDSTYLRLAPDRFAYLFLVTDVYARRIVG
WHLSQDLSHHSALQALHHAIQTIGAADGVLHHSDRGSQYCCHAYRRALAAARLLPSMTDANHCYQNAIAERVNGILKDEFDLDAIFPSLPAAQRAVADAI
HRYNTVRLHGSLQLQTPAHVFTHAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1172 bp | 390 aa | 57 | 1228 | + | Yes |
Chemistry : DDE
ORF sequence :
MERSRRQFSPELKQRIVEELEAGHLSVREAARTVQTSAAMVHHWLEEFGRYRPKRDVVEVVMKSEQERIAALEKALAEAHLTLQVYDELITQANRVYKTD
LKKKLWYNTARACCDERALPVRAVCARLGRTRDAYYKRAARVGGGERGASKAVVLAQVAVVRAAQPRVGTRKLHTHLAAAGVAVGRDRLFTWLRAAAALV
PRKRRRTFTTYSKHGYVVAPNRLKDAEITGPRQAVVSDSTYLRLAPDRFAYLFLVTDVYARRIVGWHLSQDLSHHSALQALHHAIQTIGAADGVLHHSDR
GSQYCCHAYRRALAAARLLPSMTDANHCYQNAIAERVNGILKDEFDLDAIFPSLPAAQRAVADAIHRYNTVRLHGSLQLQTPAHVFTHAA
LKKKLWYNTARACCDERALPVRAVCARLGRTRDAYYKRAARVGGGERGASKAVVLAQVAVVRAAQPRVGTRKLHTHLAAAGVAVGRDRLFTWLRAAAALV
PRKRRRTFTTYSKHGYVVAPNRLKDAEITGPRQAVVSDSTYLRLAPDRFAYLFLVTDVYARRIVGWHLSQDLSHHSALQALHHAIQTIGAADGVLHHSDR
GSQYCCHAYRRALAAARLLPSMTDANHCYQNAIAERVNGILKDEFDLDAIFPSLPAAQRAVADAIHRYNTVRLHGSLQLQTPAHVFTHAA
Blast result :
Comments
ISGau1 is 52% aa similar to ISDde2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Ichikawa, N. (2010) Direct submission
2] Oguchi,A., Ankai,A., Yashiro,I., Takahashi,M., Terui,Y., Fukui,S., Yokoyama,H., Ichikawa,N., Takasaki,K., Miura,H., Matsushita,S., Watanabe,Y., Sekiguchi,Y., Nakamura,K., Tanikawa,S., Hanada,S., Kamagata,Y., Fujita,N. and Kikuchi,H. (2006) Direct submission Genbank
2] Oguchi,A., Ankai,A., Yashiro,I., Takahashi,M., Terui,Y., Fukui,S., Yokoyama,H., Ichikawa,N., Takasaki,K., Miura,H., Matsushita,S., Watanabe,Y., Sekiguchi,Y., Nakamura,K., Tanikawa,S., Hanada,S., Kamagata,Y., Fujita,N. and Kikuchi,H. (2006) Direct submission Genbank