ISPha1
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| GU826204 | Y | Paracoccus halophilus | Paracoccus halophilus JCM 14014 |
DNA section
IS Length : 842 bp
Ends
IR Length : 12/13
IRL : GGTCAGGACCCATAAGGCCGCTTGAACCCTTTGGATAGTCGTGATTCACG
IRR : GGTCAGGACTCATTTTGGCAGCCAAAAGATGACGATGGCGGCGATGCAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGGCCACTGA | CTAG | CGATAACTTT | 4 |
DNA sequence
GGTCAGGACCCATAAGGCCGCTTGAACCCTTTGGATAGTCGTGATTCACGCCCGCAAACTACGGGGGCAACATGAGCGACTACTACTGGTTGACGGAGGC
GCAGATGGAGCGTCTTCGACCGCACTTTCCGAAATCACGTGGCGTACCAAGGGTCGATGATCGGCGGGTGCTGAGTGGGATCATCTACGTCAAACGGAAC
GGTTTGCAGTGGCGCGACGCGCCATCGGTGTATGGCCCGCACAAGACGCTCTACAACCGGTTCGTCAGATGGTCTCGTATGGGTGTGTTCGCACGCATCT
TCAAGGACTTGGCGCAACCAGGGCGAGACGGAGACACCATCATGATAGACAGTACCCACATGAAGGCGCACCGCACGGCGGCAAGCCTCTCAAAAAGGGG
GATCAAGCCGAGGGCCATCGGGCGGACCAAAGGCGGGCTGAACTCAAAGCTGCACATGGTGTCGGACGGTCGTGGCCGCCCGCTGACGTTCTTTCTGTCG
CCGGGCCAGATGAGTGATGCGCGTGGCGCTGTCGTCCTGTTGGGTGATCTGCCGAAGGCGAAACGGCTGCTCGGTGACAAAGGGTACGATGCCGACTGGT
TTCGCGAAGAACTGAAGCGCATGGGTATGCGCACCTGCATCCCGCCTCGGACAAAGCGAAAGAGGCCCGCAACCTACAACAAGCGCCTCTACAGAAAGCG
TCACCGTATCGAGAATGCCTTTGCCCGCTTGAAAGATTGGCGGCGTATCGCAACCCGTTATGACAGATGCGGAGACCTGTTCCTCTCCGCCATCTGCATC
GCCGCCATCGTCATCTTTTGGCTGCCAAAATGAGTCCTGACC
GCAGATGGAGCGTCTTCGACCGCACTTTCCGAAATCACGTGGCGTACCAAGGGTCGATGATCGGCGGGTGCTGAGTGGGATCATCTACGTCAAACGGAAC
GGTTTGCAGTGGCGCGACGCGCCATCGGTGTATGGCCCGCACAAGACGCTCTACAACCGGTTCGTCAGATGGTCTCGTATGGGTGTGTTCGCACGCATCT
TCAAGGACTTGGCGCAACCAGGGCGAGACGGAGACACCATCATGATAGACAGTACCCACATGAAGGCGCACCGCACGGCGGCAAGCCTCTCAAAAAGGGG
GATCAAGCCGAGGGCCATCGGGCGGACCAAAGGCGGGCTGAACTCAAAGCTGCACATGGTGTCGGACGGTCGTGGCCGCCCGCTGACGTTCTTTCTGTCG
CCGGGCCAGATGAGTGATGCGCGTGGCGCTGTCGTCCTGTTGGGTGATCTGCCGAAGGCGAAACGGCTGCTCGGTGACAAAGGGTACGATGCCGACTGGT
TTCGCGAAGAACTGAAGCGCATGGGTATGCGCACCTGCATCCCGCCTCGGACAAAGCGAAAGAGGCCCGCAACCTACAACAAGCGCCTCTACAGAAAGCG
TCACCGTATCGAGAATGCCTTTGCCCGCTTGAAAGATTGGCGGCGTATCGCAACCCGTTATGACAGATGCGGAGACCTGTTCCTCTCCGCCATCTGCATC
GCCGCCATCGTCATCTTTTGGCTGCCAAAATGAGTCCTGACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 762 bp | 253 aa | 72 | 833 | + | No |
Chemistry : DDE
ORF sequence :
MSDYYWLTEAQMERLRPHFPKSRGVPRVDDRRVLSGIIYVKRNGLQWRDAPSVYGPHKTLYNRFVRWSRMGVFARIFKDLAQPGRDGDTIMIDSTHMKAH
RTAASLSKRGIKPRAIGRTKGGLNSKLHMVSDGRGRPLTFFLSPGQMSDARGAVVLLGDLPKAKRLLGDKGYDADWFREELKRMGMRTCIPPRTKRKRPA
TYNKRLYRKRHRIENAFARLKDWRRIATRYDRCGDLFLSAICIAAIVIFWLPK
RTAASLSKRGIKPRAIGRTKGGLNSKLHMVSDGRGRPLTFFLSPGQMSDARGAVVLLGDLPKAKRLLGDKGYDADWFREELKRMGMRTCIPPRTKRKRPA
TYNKRLYRKRHRIENAFARLKDWRRIATRYDRCGDLFLSAICIAAIVIFWLPK
Blast result :
Comments
ISPha1 has been identified by its transposition into entrapment vector pMEC1 (cI-tetA cassette). ISPha1 is 63% (ORF1) aa identical to ISPaes2. As judged from DNA hybridization analysis ISPha1 is present in 6 copies in the genome of P. halophilus JCM 14014. Homologous sequence was also detected by DNA hybridization in P. homiensis DSM 17862.
References
1] Dziewit, L. and Bartosik, D. (2010) Direct submission
2] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277
2] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277