ISPa30
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DQ315788 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa |
DNA section
IS Length : 2436 bp
Ends
IR Length : 20/24
IRL : GTAAGCGTAACACGAACCCCACATTCCAGTTTTAGCTAATCCACCATACC
IRR : GTAAGCGTAACAACAACCACACCTTGCGGCATGGTTGTTGTTTCGTTTAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTGTGTTGGTG | AAATAAAA | CGACATCCAGTT | 8 |
DNA sequence
GTAAGCGTAACACGAACCCCACATTCCAGTTTTAGCTAATCCACCATACCCTGTTTGTATCATTCACCAAGCAGGGTTTCTTATGATCCGCAGAACGTCC
AAACAATGGCTGGCGCTGTTTGACCAGTGCAAAGCCAGCGGTTTAACTCAGACCGAATTTGCTAAGCAGCATGGTATCGACCCAAGTTACTTTAGCTTTC
GCAAGCGGCAGTTGCTTGCCTGGCAGCAGGACGACAATGACGGCTTTGTCGAATTAACACCGCACGGCATGATGCTTTGCCAGCCGATGTTGCTCAGGAA
GCGGGATATTGAACTGCACCTGCCTGCTGGCACCGATGCCGTGTGGCTCGCCACCTTGCTCAAGGCGATGTCGTGATGAAGATGTTTATCGATGTGCCAA
AAATCTATCTGCACAAACAGCCTGTCGATTTTAGAAAATCCATCAATGGCTTGTCCTTGTTGGTTGAGCAGGAAATGCAGCTGTCGCCCTTTAGCGGGGC
GCTCTTTGTATTCTGTAACCAACAGCGTTCGCGCTTGAAAGTGCTGTACTGGGACCAGACTGGCTTTTGCCTGTGGTACAAACGATTGGAAAAAGACAAA
TTCCGCTGGCCACGTCTGATGTCGGGTAAGGTAGTCTCCATCAGCGAACAGCAGTGGCACTGGCTGCTACAAGGCATTGATATCCAAAAACTAACTCCCC
ATCAGCCGCTGAGTTACCAAAGCGCGGGCTGATATGCAGATCCGCTATAAATCCTTCGGATCGCAGTTGAAACAATCTAAATCAGCACAAGCACTTAGCC
AAACGGGTCGTTACAATAACGGCCATGAAAAAGAAATCTACACCACTTCCGGACGATGTTGAACTGCTCAAGAAAATGCTGCTTGAGCAACAGGCCAAAT
TAGAACGCCAACTCGCCCAGAAAGACAAACGCATCGTCAAATTAGAGACGCTCAATCAAAAACTGACTGAGCGCATTCAGCGCTTTGAAGAAGTCCTGAA
TCTGGCGCAGCACAAGCGGTTTAGCCCCAGCAGCGAAACCTACGAAGGCGATGGTCAGGTCTTTAACGAAGCGGAACACATTGCTGAGACTGACGTTGAG
CAGCCATCGGAAACACAAGCGCCAGCCGACGAAACGACAGATAAACAGCCTGCCAAACCGCGTCGTCCTCGCATTACCCCAGAGTTGCCGCGTATTGAAG
TGCTGCACGATATCGACACCAAAACCTGCACCTGCTGCGGGAATGAATTGCATCGGATGGGCGAGGAAGTCAGCGAGCAGATTGAGTTTATCCCCGCCGA
AATCCGCGTGATAAAACACGTACGTCCAAAATACAGCTGCCGCACCTGTGAGCGTGAAGGCACTGAAGTGGCGATACAGATTGCCGATGTCCCTGACACC
CTCTTCCCCAAAAGTATGGCGACCCCCAGCTTAGTGGCTCAAATCATCAGCGCGAAAATGCACTATGGCTTGCCTCTGTACCGGCAGGAAAAGATGTTTA
GCGAGGCAGGCATTGAGCTGAGCCGCCAGACCATGAGCCGTTGGCTGATTAGTTGCGCCGACAAGCTGGAACCGTTGTTAGCGCTGATGAAAACGGAACT
GTTAAAGCAACCGGCACTGTGGGCCGACGAGACCACATTAGATGTGCTGGATGTGGATAAAAGCACCTGTTACATGTGGGTGTACGGCTGTGGCGTAGAA
GGCTCAGCCGGTGCAAAACTGGTGTTGTTCGATTACCAGGATGGCCGCAGCGGTCAGCATGCCGTGCAGTTCCTTGATGGCTACAACGGCTATCTACAAG
TCGATGGGTATGCCGGTTATGAACAAACATCCGCTACCTTAGCTGGTTGTTGGGCACATGCGCGTCGTAAGTTTATCGAAGCGCAGCAGGCGCAAGGCAA
AGGAAAAACGGGCAAAGCCGACTGGGCCATCAACCACATCCAGAAGTTATATGCACTGGAGAGCAAACTGAAAACCCAGTCAGCACAAATAAAACAGCAG
CAACGCCAACAGCTTGCTGCACCGCTCCTGAAACAACTCTGGGATTGGCTGGAAAAATCCAAAGACACCATCCCCAAAGAAAGTCTGGTCAGCAAAGCCA
TCAGCTACACACAAAAACAGTGGCCGAATTTGATACGCTATCTGGACGATGGCCAACTGAACATCGACAACAACAGAGCCGAGCGGGCAGTGAAGCCCTT
CGTGATTGGTCGTAAAGCCTGGTTGTTTGCCAACACCAAAACCGGAGCCAAAGCCAGTGCTGCACTGTACAGTCTGGTCGAAACGGCCAAAATCAACGGC
TTAGAGCCTTATCACTATCTAACACAGCTGTTTGAGCAACTGCCAACAGCAAACACGCCTGAAGCATTAGCTAAACTGCTGCCCTACTAAACGAAACAAC
AACCATGCCGCAAGGTGTGGTTGTTGTTACGCTTAC
AAACAATGGCTGGCGCTGTTTGACCAGTGCAAAGCCAGCGGTTTAACTCAGACCGAATTTGCTAAGCAGCATGGTATCGACCCAAGTTACTTTAGCTTTC
GCAAGCGGCAGTTGCTTGCCTGGCAGCAGGACGACAATGACGGCTTTGTCGAATTAACACCGCACGGCATGATGCTTTGCCAGCCGATGTTGCTCAGGAA
GCGGGATATTGAACTGCACCTGCCTGCTGGCACCGATGCCGTGTGGCTCGCCACCTTGCTCAAGGCGATGTCGTGATGAAGATGTTTATCGATGTGCCAA
AAATCTATCTGCACAAACAGCCTGTCGATTTTAGAAAATCCATCAATGGCTTGTCCTTGTTGGTTGAGCAGGAAATGCAGCTGTCGCCCTTTAGCGGGGC
GCTCTTTGTATTCTGTAACCAACAGCGTTCGCGCTTGAAAGTGCTGTACTGGGACCAGACTGGCTTTTGCCTGTGGTACAAACGATTGGAAAAAGACAAA
TTCCGCTGGCCACGTCTGATGTCGGGTAAGGTAGTCTCCATCAGCGAACAGCAGTGGCACTGGCTGCTACAAGGCATTGATATCCAAAAACTAACTCCCC
ATCAGCCGCTGAGTTACCAAAGCGCGGGCTGATATGCAGATCCGCTATAAATCCTTCGGATCGCAGTTGAAACAATCTAAATCAGCACAAGCACTTAGCC
AAACGGGTCGTTACAATAACGGCCATGAAAAAGAAATCTACACCACTTCCGGACGATGTTGAACTGCTCAAGAAAATGCTGCTTGAGCAACAGGCCAAAT
TAGAACGCCAACTCGCCCAGAAAGACAAACGCATCGTCAAATTAGAGACGCTCAATCAAAAACTGACTGAGCGCATTCAGCGCTTTGAAGAAGTCCTGAA
TCTGGCGCAGCACAAGCGGTTTAGCCCCAGCAGCGAAACCTACGAAGGCGATGGTCAGGTCTTTAACGAAGCGGAACACATTGCTGAGACTGACGTTGAG
CAGCCATCGGAAACACAAGCGCCAGCCGACGAAACGACAGATAAACAGCCTGCCAAACCGCGTCGTCCTCGCATTACCCCAGAGTTGCCGCGTATTGAAG
TGCTGCACGATATCGACACCAAAACCTGCACCTGCTGCGGGAATGAATTGCATCGGATGGGCGAGGAAGTCAGCGAGCAGATTGAGTTTATCCCCGCCGA
AATCCGCGTGATAAAACACGTACGTCCAAAATACAGCTGCCGCACCTGTGAGCGTGAAGGCACTGAAGTGGCGATACAGATTGCCGATGTCCCTGACACC
CTCTTCCCCAAAAGTATGGCGACCCCCAGCTTAGTGGCTCAAATCATCAGCGCGAAAATGCACTATGGCTTGCCTCTGTACCGGCAGGAAAAGATGTTTA
GCGAGGCAGGCATTGAGCTGAGCCGCCAGACCATGAGCCGTTGGCTGATTAGTTGCGCCGACAAGCTGGAACCGTTGTTAGCGCTGATGAAAACGGAACT
GTTAAAGCAACCGGCACTGTGGGCCGACGAGACCACATTAGATGTGCTGGATGTGGATAAAAGCACCTGTTACATGTGGGTGTACGGCTGTGGCGTAGAA
GGCTCAGCCGGTGCAAAACTGGTGTTGTTCGATTACCAGGATGGCCGCAGCGGTCAGCATGCCGTGCAGTTCCTTGATGGCTACAACGGCTATCTACAAG
TCGATGGGTATGCCGGTTATGAACAAACATCCGCTACCTTAGCTGGTTGTTGGGCACATGCGCGTCGTAAGTTTATCGAAGCGCAGCAGGCGCAAGGCAA
AGGAAAAACGGGCAAAGCCGACTGGGCCATCAACCACATCCAGAAGTTATATGCACTGGAGAGCAAACTGAAAACCCAGTCAGCACAAATAAAACAGCAG
CAACGCCAACAGCTTGCTGCACCGCTCCTGAAACAACTCTGGGATTGGCTGGAAAAATCCAAAGACACCATCCCCAAAGAAAGTCTGGTCAGCAAAGCCA
TCAGCTACACACAAAAACAGTGGCCGAATTTGATACGCTATCTGGACGATGGCCAACTGAACATCGACAACAACAGAGCCGAGCGGGCAGTGAAGCCCTT
CGTGATTGGTCGTAAAGCCTGGTTGTTTGCCAACACCAAAACCGGAGCCAAAGCCAGTGCTGCACTGTACAGTCTGGTCGAAACGGCCAAAATCAACGGC
TTAGAGCCTTATCACTATCTAACACAGCTGTTTGAGCAACTGCCAACAGCAAACACGCCTGAAGCATTAGCTAAACTGCTGCCCTACTAAACGAAACAAC
AACCATGCCGCAAGGTGTGGTTGTTGTTACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 83 | 376 | + | No |
AG : IS66 TnpA
ORF sequence :
MIRRTSKQWLALFDQCKASGLTQTEFAKQHGIDPSYFSFRKRQLLAWQQDDNDGFVELTPHGMMLCQPMLLRKRDIELHLPAGTDAVWLATLLKAMS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 357 bp | 118 aa | 376 | 732 | + | No |
AG : IS66 TnpB
ORF sequence :
MKMFIDVPKIYLHKQPVDFRKSINGLSLLVEQEMQLSPFSGALFVFCNQQRSRLKVLYWDQTGFCLWYKRLEKDKFRWPRLMSGKVVSISEQQWHWLLQG
IDIQKLTPHQPLSYQSAG
IDIQKLTPHQPLSYQSAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1566 bp | 521 aa | 825 | 2390 | + | No |
Chemistry : DDE
ORF sequence :
MKKKSTPLPDDVELLKKMLLEQQAKLERQLAQKDKRIVKLETLNQKLTERIQRFEEVLNLAQHKRFSPSSETYEGDGQVFNEAEHIAETDVEQPSETQAP
ADETTDKQPAKPRRPRITPELPRIEVLHDIDTKTCTCCGNELHRMGEEVSEQIEFIPAEIRVIKHVRPKYSCRTCEREGTEVAIQIADVPDTLFPKSMAT
PSLVAQIISAKMHYGLPLYRQEKMFSEAGIELSRQTMSRWLISCADKLEPLLALMKTELLKQPALWADETTLDVLDVDKSTCYMWVYGCGVEGSAGAKLV
LFDYQDGRSGQHAVQFLDGYNGYLQVDGYAGYEQTSATLAGCWAHARRKFIEAQQAQGKGKTGKADWAINHIQKLYALESKLKTQSAQIKQQQRQQLAAP
LLKQLWDWLEKSKDTIPKESLVSKAISYTQKQWPNLIRYLDDGQLNIDNNRAERAVKPFVIGRKAWLFANTKTGAKASAALYSLVETAKINGLEPYHYLT
QLFEQLPTANTPEALAKLLPY
ADETTDKQPAKPRRPRITPELPRIEVLHDIDTKTCTCCGNELHRMGEEVSEQIEFIPAEIRVIKHVRPKYSCRTCEREGTEVAIQIADVPDTLFPKSMAT
PSLVAQIISAKMHYGLPLYRQEKMFSEAGIELSRQTMSRWLISCADKLEPLLALMKTELLKQPALWADETTLDVLDVDKSTCYMWVYGCGVEGSAGAKLV
LFDYQDGRSGQHAVQFLDGYNGYLQVDGYAGYEQTSATLAGCWAHARRKFIEAQQAQGKGKTGKADWAINHIQKLYALESKLKTQSAQIKQQQRQQLAAP
LLKQLWDWLEKSKDTIPKESLVSKAISYTQKQWPNLIRYLDDGQLNIDNNRAERAVKPFVIGRKAWLFANTKTGAKASAALYSLVETAKINGLEPYHYLT
QLFEQLPTANTPEALAKLLPY
Blast result :
Comments
ISPa30 is 51% (ORFA), 78% (ORFB) and 63% (ORFC : the transposase) aa similar to ISVsa2.
ISPa30 is disrupted by insertion of ISPa31. The sequence was reconstructed in silico by deletion of ISPa31.
ISPa30 is disrupted by insertion of ISPa31. The sequence was reconstructed in silico by deletion of ISPa31.
References
1] Naas,T., Poirel,L. and Nordmann,P. (1999) Biochim. Biophys. Acta 1489 (2-3), 445-451
2] Naas,T., Aubert,D., Lambert,T. and Nordmann,P. (2006) Antimicrob. Agents Chemother. 50 (5), 1745-1752
2] Naas,T., Aubert,D., Lambert,T. and Nordmann,P. (2006) Antimicrob. Agents Chemother. 50 (5), 1745-1752