ISAcsp1
- Family Tn3
- Group
Isoform Synonym(s) TnAcsp1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Acinetobacter species | Acinetobacter species EB104 plasmid pAC450 |
DNA section
IS Length : 3736 bp
Ends
IR Length : 45/46
IRL : GGGGTCGTCTCAGGAAACGGAAAATATAGCACGCTAAGGATTTTCACTCC
IRR : GGGGTCGTCTCAGGAAACGGAAAATATAGCACGCTAAGGTTTTTCAATTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGAATACGCTTATT | TATATATACAGGCTT | 0 |
DNA sequence
GGGGTCGTCTCAGGAAACGGAAAATATAGCACGCTAAGGATTTTCACTCCGACTTCTTTTAACGATATCCAACACGGTATTTTTGCTTAGATTGACCAGC
CGACCGACTTGTCGATAAGAGTGTCCAGCAGACACCAACTCCAACACTTTAGGTGCCAGACGATCTGACTTGGTACGTTGACCAGGACGACGACCAAACA
CAACGCCCCGCGCTTGTGCCGCTGCTACACCAGAGCGCACACGTTCACGTAGTAAATCTCCCTCAAACTCTGCTAATGCAGACATCACAGAAGCCATGAG
CTTTCCCTGAGATGTGGAAAGGTCAAACTGCAAACCTGTTTGTGCCACCAGAGATACCCCGAAATGCCCAAGATCTTGTAATGACTGTACGAGATCCACG
GTGCTTCTGCCCCAGCGTGTCAATTCCGTCACCAAGATGGCATCGATTTCACGAGATTGCGCCATGGTCATCAAGTATTTTCTTTGCAATCTCTCATCCT
TACTGCCTGATGCAGTTTCTTTCCAAACACCGACAACATCGTAGCCGCAACGATTGGCGTAGGCACGTAGGTCATATTCCTGTCGTTCGCAGGACTGATC
GTTCGTTGATACTCGGCAATAAATGGCCGCATTCTGTCCCAATTGAACCTCCTTGCGAAATTGCAGCGCAAAGCTAAGCGTGGCGGTACAAAATAATGTA
CCTTTAATAGTTTAGTTTATTTGGGACTAATGAGCCATGCCGCGCCATTCTATTTTGTCGTCCACGGAATTGGGAAGCCTGTTCGAAATTCCAGATGATA
GAGCTTTTATGCTTCAGCATTACACTCTGAGTGATTCTGATAAATCCATAGTCCATCAACATCGAGGGGCATCCAACAAGCTAGGCTTTGCAATTCAACT
TTGCTACATGCGTTACCCTGGAGTGATATTGGGTATTAACGACGAACCATTCAAACCCTTACTTTTCATGATGGCTGCTCAACTTAGTATATCAGTGGAC
TGCTGGAATGATTATGGTCAGCGCGAGCAAACAAGACGAGAACATATTGCCGAATTGAAAAAAGTTTTTGGGTTCAAACTATTCACCTTGAGTGACTATC
GGCAATCGGTCAATATGATGGTGGATTTGTCCCTTCAGACAGATAAAGGTCTTATTTTAGCCACAACTCTCGTTGAAAATTTACGACAAAAATCAGTCCT
TTTGCCAGCTATAAATGCAATTGATAGTATTTGCGCAGAAGCAATCACATCTGCCAACCGCATAATTTATACTGCTTTGACGAGCTCACTTTCAGAAACT
CATCGTCAAAATATAGATGCTTTGCTGAACCGTAAAGAGGGCAGCAAACTGACAATATTGGGGTGGCTTAGACAGTCACCAGCTAAACCAAACTCAAAGT
ACATGCTTGAGCACATTGAGCGGCTGAAGTGCTTACAAGCCATCGATCTACCTGAAGACATTGGAAAACACGTTCATCAAAATCGCTTACTGAAAATTGC
TCGCGAAGGTGGGCAAATGACTCCTGCTGATTTGGCCAGATTTGAATCTCAACGTCGATATGCCACTTTGGTAGCAATCGTTGTGGAGAGTATGGCCACG
ATCACCGACGAAATTATTGACCTGCATGATCGCATTATTGGAAAGCTATTTGCTATCGCAAAAAATAAACACCAGCAACAATTTCAATCATCAGGTAAAG
CGATCAACGACAAAGTGCGTTTGTATGGGCTTATCGGGAAAGCCTTGCTAGATGCCAAGCAAAATGGCAGTGATCCGTTTGCCGCCATCGAAACTGTTAT
TTCATGGGATGCTTTTGCAGCAAGTATTACTGAGGCAGAAAAACTGGCGCAACCCGAAGACTTTGATTTTTTACCACGAATTGGCGAGAGCTATGCAACT
TTACGCCGTTACGCGCCAGAACTTCTTGCCATACTTAAATTGCGTGCTGCCCCAGCAGCAAAAGATATGCTTGCCGCTGTAGAGTTGCTCCGCAGCATGA
ATGTTGATAATACACGAAAAATCCCCTCTAATGCTCCAATAGCGTTTATCAAAAAACGGTGGGCAAGACTAGTCTTTACCGATGATGGTATTGATCGCCG
CTATTACGAAATATGTGTGTTATCTGAACTTAAAAACTCGCTACGGGCTGGGGATCTCTGGGTACAAGGCTCTCGCCAATTCAAAGACTTCGATGAGTAT
TTAGTGACTGCTGATAAATTTAATCAGCTAAAACAGTCCAATGAGTTACCTCTAACCATTACTACTGATTGTGATCTATATTTGCAAAGTCGCCTTTCAC
TATTGGAGCAGAAATTAGCAATCATTAATCGCATGGCAGCGACCAACGATTTGCCTGATGCGATGATCAATCAATCTGGTCTAAAAATAACACCTCTCGA
TGCCGTGGCTCCTGATACAGCCCAAACTTTAATTGACCAAACAGCAATGCTGTTACCACATATCAAGATCACCGAATTATTAATGGAGGTCGATGAATGG
ACAGGCTTTACTCGACACTTCACTCATTTGAAAACGGATGACACTGCGAAGGATAAAGCGTTATTGCTCACGACCATACTGGCTGATGCAATTAATTTAG
GGCTGACGAAAATGGCCGAATCATGTCCTGGTACAACTTATGCCAAACTCTCATGGCTACAGGCTTGGCATATCCGTGATGAAACTTATTCAACGGCATT
GGCCGAATTGGTGAATGCTCAATTGAAACAACCTTTCGCTGAAAACTGGGGAGATGGCACTACCTCCTCATCAGATGGCCAACGCTTCCGCGCAGGTGGC
AGAGCTGAAAGTACAGGACATATCAATCCAAAATATGGCGCAGAGCCTGGAAGGTTATTTTATACACACATTTCTGATCAATACGCACCATTTAGCAGTA
AGCTGATCAATGTGGGCGTTCGTGATTCAACCTATGTTCTTGATGGGCTGCTGTATCACGAGTCTGATTTACGCATCGAGGAACACTACACCGATACGGC
TGGATTCACCGACCATGTTTTTGCACTCATGCACATGCTAGGGTTTCGATTTGCACCACGAATTCGTGATCTGGGCGATACGAAACTCTATATTCCAAAG
TCAGACATAGACTATGCCGCACTTAAACCCATGATTGGCGGCACACTGAATATCAAACAGATTCGTACTCATTGGGACGATATTTTGCGTCTGGCCGCAT
CAATCAAGCAAGGGACTGTCACAGCGTCGCTAATGCTACGTAAACTCGGTAGTTATCCACGACAGAATGGTTTGGCATTGGCATTACGAGAGCTGGGGCG
TATTGAGCGCACACTGTTTATCTTGGATTGGTTGCAAAGCGTAGAACTACGCCGTCGGGTTCAAGCTGGATTAAATAAAGGTGAAGCCCGTAATGCATTA
GCGCGCGCCGTTTTCTTTTATCGATTGGGTGAGATTCGAGATCGAAGCTTTGAGCAACAGCGTTATCGAGCCAGTGGCTTGAATCTAGTGACCGCCGCCA
TTGTATTGTGGAACACGGTTTATTTAGAGAGAGCGACTAACGCCCTACGAGGGCATGGTCGCACAGTCGATGATACCTTGCTGCAATATCTGTCACCGCT
GGGGTGGGAGCACATCAATTTAACTGGGGATTATCTATGGCGAAGCAAAGCCAAGATAGGTGGTGGAAAATTTAGGCCATTGCGCGCAATTGAAAAACCT
TAGCGTGCTATATTTTCCGTTTCCTGAGACGACCCC
CGACCGACTTGTCGATAAGAGTGTCCAGCAGACACCAACTCCAACACTTTAGGTGCCAGACGATCTGACTTGGTACGTTGACCAGGACGACGACCAAACA
CAACGCCCCGCGCTTGTGCCGCTGCTACACCAGAGCGCACACGTTCACGTAGTAAATCTCCCTCAAACTCTGCTAATGCAGACATCACAGAAGCCATGAG
CTTTCCCTGAGATGTGGAAAGGTCAAACTGCAAACCTGTTTGTGCCACCAGAGATACCCCGAAATGCCCAAGATCTTGTAATGACTGTACGAGATCCACG
GTGCTTCTGCCCCAGCGTGTCAATTCCGTCACCAAGATGGCATCGATTTCACGAGATTGCGCCATGGTCATCAAGTATTTTCTTTGCAATCTCTCATCCT
TACTGCCTGATGCAGTTTCTTTCCAAACACCGACAACATCGTAGCCGCAACGATTGGCGTAGGCACGTAGGTCATATTCCTGTCGTTCGCAGGACTGATC
GTTCGTTGATACTCGGCAATAAATGGCCGCATTCTGTCCCAATTGAACCTCCTTGCGAAATTGCAGCGCAAAGCTAAGCGTGGCGGTACAAAATAATGTA
CCTTTAATAGTTTAGTTTATTTGGGACTAATGAGCCATGCCGCGCCATTCTATTTTGTCGTCCACGGAATTGGGAAGCCTGTTCGAAATTCCAGATGATA
GAGCTTTTATGCTTCAGCATTACACTCTGAGTGATTCTGATAAATCCATAGTCCATCAACATCGAGGGGCATCCAACAAGCTAGGCTTTGCAATTCAACT
TTGCTACATGCGTTACCCTGGAGTGATATTGGGTATTAACGACGAACCATTCAAACCCTTACTTTTCATGATGGCTGCTCAACTTAGTATATCAGTGGAC
TGCTGGAATGATTATGGTCAGCGCGAGCAAACAAGACGAGAACATATTGCCGAATTGAAAAAAGTTTTTGGGTTCAAACTATTCACCTTGAGTGACTATC
GGCAATCGGTCAATATGATGGTGGATTTGTCCCTTCAGACAGATAAAGGTCTTATTTTAGCCACAACTCTCGTTGAAAATTTACGACAAAAATCAGTCCT
TTTGCCAGCTATAAATGCAATTGATAGTATTTGCGCAGAAGCAATCACATCTGCCAACCGCATAATTTATACTGCTTTGACGAGCTCACTTTCAGAAACT
CATCGTCAAAATATAGATGCTTTGCTGAACCGTAAAGAGGGCAGCAAACTGACAATATTGGGGTGGCTTAGACAGTCACCAGCTAAACCAAACTCAAAGT
ACATGCTTGAGCACATTGAGCGGCTGAAGTGCTTACAAGCCATCGATCTACCTGAAGACATTGGAAAACACGTTCATCAAAATCGCTTACTGAAAATTGC
TCGCGAAGGTGGGCAAATGACTCCTGCTGATTTGGCCAGATTTGAATCTCAACGTCGATATGCCACTTTGGTAGCAATCGTTGTGGAGAGTATGGCCACG
ATCACCGACGAAATTATTGACCTGCATGATCGCATTATTGGAAAGCTATTTGCTATCGCAAAAAATAAACACCAGCAACAATTTCAATCATCAGGTAAAG
CGATCAACGACAAAGTGCGTTTGTATGGGCTTATCGGGAAAGCCTTGCTAGATGCCAAGCAAAATGGCAGTGATCCGTTTGCCGCCATCGAAACTGTTAT
TTCATGGGATGCTTTTGCAGCAAGTATTACTGAGGCAGAAAAACTGGCGCAACCCGAAGACTTTGATTTTTTACCACGAATTGGCGAGAGCTATGCAACT
TTACGCCGTTACGCGCCAGAACTTCTTGCCATACTTAAATTGCGTGCTGCCCCAGCAGCAAAAGATATGCTTGCCGCTGTAGAGTTGCTCCGCAGCATGA
ATGTTGATAATACACGAAAAATCCCCTCTAATGCTCCAATAGCGTTTATCAAAAAACGGTGGGCAAGACTAGTCTTTACCGATGATGGTATTGATCGCCG
CTATTACGAAATATGTGTGTTATCTGAACTTAAAAACTCGCTACGGGCTGGGGATCTCTGGGTACAAGGCTCTCGCCAATTCAAAGACTTCGATGAGTAT
TTAGTGACTGCTGATAAATTTAATCAGCTAAAACAGTCCAATGAGTTACCTCTAACCATTACTACTGATTGTGATCTATATTTGCAAAGTCGCCTTTCAC
TATTGGAGCAGAAATTAGCAATCATTAATCGCATGGCAGCGACCAACGATTTGCCTGATGCGATGATCAATCAATCTGGTCTAAAAATAACACCTCTCGA
TGCCGTGGCTCCTGATACAGCCCAAACTTTAATTGACCAAACAGCAATGCTGTTACCACATATCAAGATCACCGAATTATTAATGGAGGTCGATGAATGG
ACAGGCTTTACTCGACACTTCACTCATTTGAAAACGGATGACACTGCGAAGGATAAAGCGTTATTGCTCACGACCATACTGGCTGATGCAATTAATTTAG
GGCTGACGAAAATGGCCGAATCATGTCCTGGTACAACTTATGCCAAACTCTCATGGCTACAGGCTTGGCATATCCGTGATGAAACTTATTCAACGGCATT
GGCCGAATTGGTGAATGCTCAATTGAAACAACCTTTCGCTGAAAACTGGGGAGATGGCACTACCTCCTCATCAGATGGCCAACGCTTCCGCGCAGGTGGC
AGAGCTGAAAGTACAGGACATATCAATCCAAAATATGGCGCAGAGCCTGGAAGGTTATTTTATACACACATTTCTGATCAATACGCACCATTTAGCAGTA
AGCTGATCAATGTGGGCGTTCGTGATTCAACCTATGTTCTTGATGGGCTGCTGTATCACGAGTCTGATTTACGCATCGAGGAACACTACACCGATACGGC
TGGATTCACCGACCATGTTTTTGCACTCATGCACATGCTAGGGTTTCGATTTGCACCACGAATTCGTGATCTGGGCGATACGAAACTCTATATTCCAAAG
TCAGACATAGACTATGCCGCACTTAAACCCATGATTGGCGGCACACTGAATATCAAACAGATTCGTACTCATTGGGACGATATTTTGCGTCTGGCCGCAT
CAATCAAGCAAGGGACTGTCACAGCGTCGCTAATGCTACGTAAACTCGGTAGTTATCCACGACAGAATGGTTTGGCATTGGCATTACGAGAGCTGGGGCG
TATTGAGCGCACACTGTTTATCTTGGATTGGTTGCAAAGCGTAGAACTACGCCGTCGGGTTCAAGCTGGATTAAATAAAGGTGAAGCCCGTAATGCATTA
GCGCGCGCCGTTTTCTTTTATCGATTGGGTGAGATTCGAGATCGAAGCTTTGAGCAACAGCGTTATCGAGCCAGTGGCTTGAATCTAGTGACCGCCGCCA
TTGTATTGTGGAACACGGTTTATTTAGAGAGAGCGACTAACGCCCTACGAGGGCATGGTCGCACAGTCGATGATACCTTGCTGCAATATCTGTCACCGCT
GGGGTGGGAGCACATCAATTTAACTGGGGATTATCTATGGCGAAGCAAAGCCAAGATAGGTGGTGGAAAATTTAGGCCATTGCGCGCAATTGAAAAACCT
TAGCGTGCTATATTTTCCGTTTCCTGAGACGACCCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 615 bp | 204 aa | 648 | 34 | - | No |
AG : Tn3 resolvase
ORF sequence :
VQLGQNAAIYCRVSTNDQSCERQEYDLRAYANRCGYDVVGVWKETASGSKDERLQRKYLMTMAQSREIDAILVTELTRWGRSTVDLVQSLQDLGHFGVSL
VAQTGLQFDLSTSQGKLMASVMSALAEFEGDLLRERVRSGVAAAQARGVVFGRRPGQRTKSDRLAPKVLELVSAGHSYRQVGRLVNLSKNTVLDIVKRSR
SENP
VAQTGLQFDLSTSQGKLMASVMSALAEFEGDLLRERVRSGVAAAQARGVVFGRRPGQRTKSDRLAPKVLELVSAGHSYRQVGRLVNLSKNTVLDIVKRSR
SENP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 2967 bp | 989 aa | 737 | 3703 | + | No |
Chemistry : DDE
ORF sequence :
MPRHSILSSTELGSLFEIPDDRAFMLQHYTLSDSDKSIVHQHRGASNKLGFAIQLCYMRYPGVILGINDEPFKPLLFMMAAQLSISVDCWNDYGQREQTR
REHIAELKKVFGFKLFTLSDYRQSVNMMVDLSLQTDKGLILATTLVENLRQKSVLLPAINAIDSICAEAITSANRIIYTALTSSLSETHRQNIDALLNRK
EGSKLTILGWLRQSPAKPNSKYMLEHIERLKCLQAIDLPEDIGKHVHQNRLLKIAREGGQMTPADLARFESQRRYATLVAIVVESMATITDEIIDLHDRI
IGKLFAIAKNKHQQQFQSSGKAINDKVRLYGLIGKALLDAKQNGSDPFAAIETVISWDAFAASITEAEKLAQPEDFDFLPRIGESYATLRRYAPELLAIL
KLRAAPAAKDMLAAVELLRSMNVDNTRKIPSNAPIAFIKKRWARLVFTDDGIDRRYYEICVLSELKNSLRAGDLWVQGSRQFKDFDEYLVTADKFNQLKQ
SNELPLTITTDCDLYLQSRLSLLEQKLAIINRMAATNDLPDAMINQSGLKITPLDAVAPDTAQTLIDQTAMLLPHIKITELLMEVDEWTGFTRHFTHLKT
DDTAKDKALLLTTILADAINLGLTKMAESCPGTTYAKLSWLQAWHIRDETYSTALAELVNAQLKQPFAENWGDGTTSSSDGQRFRAGGRAESTGHINPKY
GAEPGRLFYTHISDQYAPFSSKLINVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHMLGFRFAPRIRDLGDTKLYIPKSDIDYAALKPMI
GGTLNIKQIRTHWDDILRLAASIKQGTVTASLMLRKLGSYPRQNGLALALRELGRIERTLFILDWLQSVELRRRVQAGLNKGEARNALARAVFFYRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERATNALRGHGRTVDDTLLQYLSPLGWEHINLTGDYLWRSKAKIGGGKFRPLRAIEKP
REHIAELKKVFGFKLFTLSDYRQSVNMMVDLSLQTDKGLILATTLVENLRQKSVLLPAINAIDSICAEAITSANRIIYTALTSSLSETHRQNIDALLNRK
EGSKLTILGWLRQSPAKPNSKYMLEHIERLKCLQAIDLPEDIGKHVHQNRLLKIAREGGQMTPADLARFESQRRYATLVAIVVESMATITDEIIDLHDRI
IGKLFAIAKNKHQQQFQSSGKAINDKVRLYGLIGKALLDAKQNGSDPFAAIETVISWDAFAASITEAEKLAQPEDFDFLPRIGESYATLRRYAPELLAIL
KLRAAPAAKDMLAAVELLRSMNVDNTRKIPSNAPIAFIKKRWARLVFTDDGIDRRYYEICVLSELKNSLRAGDLWVQGSRQFKDFDEYLVTADKFNQLKQ
SNELPLTITTDCDLYLQSRLSLLEQKLAIINRMAATNDLPDAMINQSGLKITPLDAVAPDTAQTLIDQTAMLLPHIKITELLMEVDEWTGFTRHFTHLKT
DDTAKDKALLLTTILADAINLGLTKMAESCPGTTYAKLSWLQAWHIRDETYSTALAELVNAQLKQPFAENWGDGTTSSSDGQRFRAGGRAESTGHINPKY
GAEPGRLFYTHISDQYAPFSSKLINVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHMLGFRFAPRIRDLGDTKLYIPKSDIDYAALKPMI
GGTLNIKQIRTHWDDILRLAASIKQGTVTASLMLRKLGSYPRQNGLALALRELGRIERTLFILDWLQSVELRRRVQAGLNKGEARNALARAVFFYRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERATNALRGHGRTVDDTLLQYLSPLGWEHINLTGDYLWRSKAKIGGGKFRPLRAIEKP
Blast result :
Comments
ISAcsp1 is 53% (ORFA : the resolvase) aa similar to ISSba14 and 83% (ORFB : the transposase) to ISSod9.
IS is part of TnAcsp1 and located on the transferrable plasmid pAC450 that provides the strain EB104 with an additional metabolic feature not observed at other Acinetobacter strains, thus the plasmid is acquired from another (original) host, which could not be determined so far.
Right IR contains at the outer end the insertion of an additional base before the last base.
Start position of ORF1 was assumed from the (C-terminal truncated) TnpR of Tn5047 (acc.No AJ251307) due to 99% nucleotide sequence identity.
Position 368-3739 and the consecutive 214bp downstream of the left IS of TnAcsp1 (but not of the corresponding region upstream of the right IS)shows 99% nucleotide sequence identity to a part of Tn5047 of pKLH201 (acc.No see above) and the 214bp downstream of that transposon (unfortunately this plasmid is not sequenced further downstream).
The region of position 1-374 is with 1 mismatch inverted repeated 704bp downstream of the left IS (491bp downstream of the end of homology to pKLH201). It contains possibly just the C-terminal part of the TnpR, which is truncated in Tn5047. The repeat possesses the according IR, but it contains the additional base found at the right IR. My question: cold one refer to the whole region (left ISAcsp1, the 214bp of pKLH201-homology, the 451bp spacer and the 374bp repeated from left end of IS) as a prolonged ISAcsp1? In my opinion, this "element" could be transferrable as well due to the flanking IR (but I do not know so well the ropes of IS elements).
Found in TnAcsp1.
IS is part of TnAcsp1 and located on the transferrable plasmid pAC450 that provides the strain EB104 with an additional metabolic feature not observed at other Acinetobacter strains, thus the plasmid is acquired from another (original) host, which could not be determined so far.
Right IR contains at the outer end the insertion of an additional base before the last base.
Start position of ORF1 was assumed from the (C-terminal truncated) TnpR of Tn5047 (acc.No AJ251307) due to 99% nucleotide sequence identity.
Position 368-3739 and the consecutive 214bp downstream of the left IS of TnAcsp1 (but not of the corresponding region upstream of the right IS)shows 99% nucleotide sequence identity to a part of Tn5047 of pKLH201 (acc.No see above) and the 214bp downstream of that transposon (unfortunately this plasmid is not sequenced further downstream).
The region of position 1-374 is with 1 mismatch inverted repeated 704bp downstream of the left IS (491bp downstream of the end of homology to pKLH201). It contains possibly just the C-terminal part of the TnpR, which is truncated in Tn5047. The repeat possesses the according IR, but it contains the additional base found at the right IR. My question: cold one refer to the whole region (left ISAcsp1, the 214bp of pKLH201-homology, the 451bp spacer and the 374bp repeated from left end of IS) as a prolonged ISAcsp1? In my opinion, this "element" could be transferrable as well due to the flanking IR (but I do not know so well the ropes of IS elements).
Found in TnAcsp1.
References
1] Ludewig, M (2003) Direct submission.