ISPpa7

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
EU909901 ND Paracoccus pantotrophus
Paracoccus pantotrophus DSM 65
DNA section
IS Length : 2731 bp

Ends


IR Length : 26/35

IRL : GTATGCGCCGTCTCCAGCCCATTGATATCGCGGGTTTCCTGCGAGCTGCG
IRR : GTAAGCGCCGTCCCCGTCCCATTGGGATTTGGGGTGCGCTCGGGAGCGCG

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GTATGCGCCGTCTCCAGCCCATTGATATCGCGGGTTTCCTGCGAGCTGCGTAGCAGCGAGCCTCTTTGTGGTTTTCTGCAAGGGGGCGATGATGGCGGAT
GGTGGCGACGGGTTCGTTGGTCGTGTCGAGGTGGTGCGGCGGACGCGGGGGTATCGGCGCTGGCCTGAGGCGGTGAAGGCCCGGATCGTGGCGGAGAGTT
TCCAGCCCGGGGTGCGAGTTGTGGATGTCGCGCAGCGGCATGATCTTGCGCCGCATCAGCTCTCGGACTGGCGGCGCCAGGCGCGACAGGGGCTTCTGGC
GCTGCCGGCGGATGCCATGGAGGCGGTAGGCGCGACCGTGGTGCCGGCGTTCGTGCCGGTGTCGGTAGATGACGAGGTGGATGCCTCGCCTGATGCCGCG
CGTGGTGCCGAAGGGGTCGTCGGGATCGAGATTGGCGACGGGATTATTGTCCGCGTGCCGGGCGACGTGTCGGCGGAGCGAGCGGCGGCGCTTGTGCGTG
CGCTGCGCAGGTCGGCATGATCGTCGCCGGGCAGCGGCTGCCGATCCTGGTGGCGACCCGACCGGTGGACTTCCGTTGCGGTCATCAGGCGCTGGCACTG
ATCGTTCAGAACACGCTGCGGCTCGATCCGCATTCCGGGGTGATGGTGGTCTTCCGATCGAAGCGCGGCGACAGATTGAAGATCCTGGTGTGGGACGGCA
CCGGCATGGTGCTGGTGTATAAGGTCCTGGAGCAGGGCAAGTTCGCCTGGCCGAAGGTTCAGGACGGGGTGATGCGGCTGTCACGGGCGCAATGCGAGGC
GCTGGTCGAGGGCCTGGACTGGCGGCGGGTGATGGCGCAGCGGGTGACGGTGCCGACTGCGGCGGGGTGACTCAGGTCGTCGTTCCGGGGTTGTTTTGTT
GGGCTTCCCTGCCGGGATCGGGTAGAAAGGGCCATGTCGCAGCCCCTCGATCTGAGCCGCTTTCCTGACCTCCCGCCCGAGGTGGTGGCGGCCTTTGCGG
CGCAGCACGAGGCGCTGGAGGCCGCCCGGTTCGAGGCCTCGGTCGAACGCGCGGCACGCCAGCACGAGCAAGCGGTGGTGGCCGAGAAGGAGGCGTTCAT
CGCCGAGCTCAAGGAGCTGGTGGCGACGCTGGAAGGCCAGATCCAGCAGTATCGCCGCGCGAAGTTCGGGCCGAAGTCCGAGAAGCTGGACCCCGCCCAG
CTGGACCTGGCACTGGAAGACCTCGAGACTGCCATCGCCGAGACCGAGGCGCGCATCGCCGCGGTCGAGGAGAAGATCGCCGCCAGCACGCTCGATCCCG
AGAAGAAAGCTCGGCGTAAACCGCGCAAGGCCCGGGCGCTGCCCGAAGGCCTGCCGCATGTGGAGCGGGTGGTCGAGCCCGACAGCATCGCCTGTCCTTG
TGGCTGTGGCGACATGGTCCGGATCGGCGAGGACCGCAGCAAGCGGCTGGATTGCATCCCCGCGCGGTATCAGGTGATCGTCACGGTGCGACCCAGATAC
GCCTGCCCCAAGGGCCGGGCCGGTGTGGTGCAGGCAAAAGCCCCGGCGCATCTGCTCGAAGGCAGCTGGCCTACCGAGGCGCTGCTCGCGCAGATCGCGG
TCGCCAAGCATTCTGAACACATGCCCTTGAACCGGCAGTCGCTGGTCATGGCCCGGCACGGGGTGCCCATCGACCGCTCGGTGCTGGCCGACTGGATGGG
CCGGACCGGCGCGCTGATCGCGCCCGTGGTCGAGCGCATGACGGTGCTTCTGAAGACCGGCGGCACCCGCCTCTATGTCGACGAGACCACAGCCCCGGTC
CTGGACCCAGGGCGCGGCAAGACGAAGACCGGATATCTCTGGGCGGTGCTACGCGACGATCGCGGTTGGGGCGGTCCCGCCCCGCCCGGCGTGGTGTTCC
ACTATCGCCCGGGCCGCGCCGGCGAGAACGCCGACGAGATCCTCGACGGGTTCGACGGGACGATTCAGGTCGACGCCTATGGCGGCTACACCCATCTGGC
CAAGCCCGACCGGAAGGGCGGCAAGCCGCTTGCGCTGGCGTTCTGCTGGTCGCACGGCCGCAGGAAGCTCATCGCGGCCAGACCGAAGGCCGGCTCGCCC
ATTGTCGACGAGGCGCTGGCGCGCATCGCCGCGCTCTATCGGATCGAGGCCGCCATCCGCGGCAAGGATGCCGGCCTGCGCCGCACCATCCGGCAGGAGC
AGTCCCGCCCCCTGGTCGATAAATTCTTCGCCTGGCTCGCCGCCCAGGCCGACCGGACCTCGCGCAAGTCGGATCTCGGCAAGGCCCTGCACTACATGCT
GCGCCGCCAGGACGGCTTCCGGCTGTTCCTCGACGATGGCCATGTCGACATGGACTCCAACCTGGTCGAGAACGCCATCCGCAGCCCGGCCATGAACCGC
CGCAATGCGCTCTTCGCCGGCCACGACGAGGGTGGCCGCAACTGGGCCCGCTTCGCCAGCCTGATCGGCACCTGCAAGCTGAACGACGTGGAGCCTTACG
CCTACCTGCGCGATCTCTTCACAAGCATCGCCACCGGCCACCTCGACAAGGACATAGACGCCTTGATGCCATGGGCCTACGCGGCAGCGACCAAGACCTC
ACAATGAGCACATCCGGCACTCCCGGCTGAGCTCATTGTGAGCTTCACCAACCGGCACTCGCCCCCCCCCCGCACCCAACTCGCGCTCCCGAGCGCACCC
CAAATCCCAATGGGACGGGGACGGCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
432 bp143 aa89520+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MMADGGDGFVGRVEVVRRTRGYRRWPEAVKARIVAESFQPGVRVVDVAQRHDLAPHQLSDWRRQARQGLLALPADAMEAVGATVVPAFVPVSVDDEVDAS
PDAARGAEGVVGIEIGDGIIVRVPGDVSAERAAALVRALRRSA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
354 bp117 aa517870+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIVAGQRLPILVATRPVDFRCGHQALALIVQNTLRLDPHSGVMVVFRSKRGDRLKILVWDGTGMVLVYKVLEQGKFAWPKVQDGVMRLSRAQCEALVEGL
DWRRVMAQRVTVPTAAG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1674 bp557 aa9342607+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQPLDLSRFPDLPPEVVAAFAAQHEALEAARFEASVERAARQHEQAVVAEKEAFIAELKELVATLEGQIQQYRRAKFGPKSEKLDPAQLDLALEDLETA
IAETEARIAAVEEKIAASTLDPEKKARRKPRKARALPEGLPHVERVVEPDSIACPCGCGDMVRIGEDRSKRLDCIPARYQVIVTVRPRYACPKGRAGVVQ
AKAPAHLLEGSWPTEALLAQIAVAKHSEHMPLNRQSLVMARHGVPIDRSVLADWMGRTGALIAPVVERMTVLLKTGGTRLYVDETTAPVLDPGRGKTKTG
YLWAVLRDDRGWGGPAPPGVVFHYRPGRAGENADEILDGFDGTIQVDAYGGYTHLAKPDRKGGKPLALAFCWSHGRRKLIAARPKAGSPIVDEALARIAA
LYRIEAAIRGKDAGLRRTIRQEQSRPLVDKFFAWLAAQADRTSRKSDLGKALHYMLRRQDGFRLFLDDGHVDMDSNLVENAIRSPAMNRRNALFAGHDEG
GRNWARFASLIGTCKLNDVEPYAYLRDLFTSIATGHLDKDIDALMPWAYAAATKTSQ

 

Blast result :
Comments
ISPpa7 is 76% (ORFA), 90% (ORFB) and 87% (ORFC, the transposase) aa similar to iSRosp4.
References
1] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277