ISfinder Mirror Site

ISNemu1B

Family IS5
Group ISL2

MGE type ISRelated element(s) :
Isoform ISNemu1ASynonym(s)

Accession number	Transposition	Origin	Host
NZ_ACDX02000023	ND	Neisseria mucosa	Neisseria mucosa ATCC 25996

DNA section

IS Length : 856 bp

Ends

IR Length : 18/19

IRL : AGACCTCTTGCGAAAGTATCCTGAAGATTTACAATTCCCAAATGAAATAC
IRR : AGACCTCTTGCGAAAATATTCATCCCATTGCATTAATCAGCCCCGCAATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGATTTTGAT	ATT	TTAAGAAAAA	3
TAATAGCGAG	ATT	CTTTTCTATC	3
ATAAGATATT		AATAAACAAG	0

DNA sequence

AGACCTCTTGCGAAAGTATCCTGAAGATTTACAATTCCCAAATGAAATACGAAAACCTCATCCAAAGAAGCGACAGGGAATTCAAACGGCTCACAGGTGT
AACGCCCGTCCTTTTTCACGAAATGCTGCAAATCACCACAGAAGCAGAAAGCCGGAAGGTCAAGTCAGGCAGGCCGCATACGCTCAGCTTGGCAGACCAA
CTGCTGCTTACCCTAAGCTATCTGCGCCATTACCATACCCAACTCGAATTGGCCGCCATCTACGGCCTTTCCGAAAGTAATGTCTGCCGCACCATCCGTA
AAACCGAGGACGCCCTCATCCGTTGCAAACGCTTCTCCCTGCCAAAGCACAAGAATCCGGGCGACCATACGGTCATCATTGACGTTACCGAAAGCCCGAT
TGAACGTCCCAAAAAAACAGCGGCAGTATTACAGCGGCAAGAAAAAGCGGCACACGGTTAAAATCCAGGTCATATACGGCAGAGAAACTGAAAAAATCAT
CAGCATCCGGACGGGGATGGGTGCTCAGCATGATATGCGTTTAGCTAGGAGGCACCTTACAGAGCTTTATCCCTACAAAATAGTCATTGCGGATAAGGGT
TATCAAGGATTGGCTAAAACCGGATTACAGACCCCGAAAAAGAAATCCAAACATCATCTGCTGAACAAACAGGATAAAGAGGCGAATAGGCGGTTAGGCA
AACTCAGAACCGTCATCGAGCACATCAATAGGAAGCTGAAAATATTCAAAATATTGTCTCTACCTTACCGCAACAGGCGGAAACGCTTCGGATTAAGGGC
AAATCTGATTGCGGGGCTGATTAATGCAATGGGATGAATATTTTCGCAAGAGGTCT

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
420 bp	139 aa	42	461	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKYENLIQRSDREFKRLTGVTPVLFHEMLQITTEAESRKVKSGRPHTLSLADQLLLTLSYLRHYHTQLELAAIYGLSESNVCRTIRKTEDALIRCKRFSL
PKHKNPGDHTVIIDVTESPIERPKKTAAVLQRQEKAAHG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
621 bp	206 aa	217	837	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AICAITIPNSNWPPSTAFPKVMSAAPSVKPRTPSSVANASPCQSTRIRATIRSSLTLPKARLNVPKKQRQYYSGKKKRHTVKIQVIYGRETEKIISIRTG
MGAQHDMRLARRHLTELYPYKIVIADKGYQGLAKTGLQTPKKKSKHHLLNKQDKEANRRLGKLRTVIEHINRKLKIFKILSLPYRNRRKRFGLRANLIAG
LINAMG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
796 bp	264 aa	42	837	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKYENLIQRSDREFKRLTGVTPVLFHEMLQITTEAESRKVKSGRPHTLSLADQLLLTLSYLRHYHTQLELAAIYGLSESNVCRTIRKTEDALIRCKRFSL
PKHKNPGDHTVIIDVTESPIERPKKQRQYYSGKKKRHTVKIQVIYGRETEKIISIRTGMGAQHDMRLARRHLTELYPYKIVIADKGYQGLAKTGLQTPKK
KSKHHLLNKQDKEANRRLGKLRTVIEHINRKLKIFKILSLPYRNRRKRFGLRANLIAGLINAMG

Blast result :

Comments

ISNemu1B is 100% aa similar to ISNemu1A.
ISNemu1B is an isoform of ISNemu1A. ISNemu1B carry a frameshift may be induced by a ponctual mutation.

References

1] ISfinder annotation (2010).

2] Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. (2009) Direct submission GenBank.