ISHwa20
- Family IS5
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FR746099 | ND | Haloquadratum walsbyi | Haloquadratum walsbyi |
DNA section
IS Length : 1075 bp
Ends
IR Length : 22/28
IRL : GAGGGTGTCATAGAACAGTTCGCATACCAAAGAGCAGGGTGAACGCTGAG
IRR : GAGGGTGCCATACAAGGGTTCTCACACCGTGATCACGGTACTTCCCGGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAAATTTCAT | TACa/gATCCAC | ACGTGATACT | 10 |
| AGACATACTC | CGGCCA | GTGTATGCAC | 6 |
| CAGCTTTAGA | TAATGTA | GGCTTTCTTT | 7 |
DNA sequence
GAGGGTGTCATAGAACAGTTCGCATACCAAAGAGCAGGGTGAACGCTGAGGTGGAATGAGTTCAGCGACCCTGCAAGATGATCCTTCGGTAGAATCGTTC
TTCAATGTCGTGGAAACGGAGACGTTGGCATTGTTCGAGCATCTCTCCTTCGAGTTTCTTGAAGAGTTCGACGTGTTCGCCCCGGCGAAGATGGGGCGAA
CACGAGATCTTGAGCCGCCCGAGATGATGCGTGGCTTTCTCCATTGCTATTACAAGAACATCTACGGCATCCGTCCGGTTGCACGGAAACTGAACAACAC
CGTTGTCTGGCTCAGCTGTGGCTTCGATCGACCGCTGTCAAGAGATGCGGTCGATCGCTTTCTCACTGATCTTGAGCACGTCGTCGACCGGGTCTTCGAC
CATCTCGTCGAGCAGGTCGCCTTGCGGGGCCTGCTCGAGTTAACGTATTCTATCGATTCAACCGATGTGAGAGCAATGCCCGCCGATCCAGACGCATCGA
AGTGCTCTGATCCAACCGATGACGAGTACTACTACGGCTACGGCTACGGCTGCACGATCGTCTCGACCGGGCAAAAGATCCCGATTGCAGCCGAGTTCAC
CGAGAGCAAACAAGCACCAGAAGAGACGGCGATGCGCGTCACGCGTGACGCACTCGCCGTCGAGAAACCGATCTGGATGCTTGGAGACAGCGCTTACGAC
ACGCTCGAGTGGCACGACCACCTGCTGACCGCAGGGGTCGTGCCAGTCGCTCCGTACAATCCACGAAACACCGACGACCCGAAAAACATCGAGTACGGGG
TAGAAGACCGCATCGAAAAACACAGCAATGACGTTCAGCTGAAGCAATCAACGCTAGACGAGACGTACAACCGCCGGAGTGGCGTCGAACGAACCAACGA
ATCAGTCAAGGGCTGCGGTCTCGGGCGAACGCACGCCCGAGGCCGCGTCCATTCACGAGCGTAGGTGTTCCTCGCGTTGTGTCTGCGCCTCGTCGTCGCA
ATCACCAACTACGAACGAGGAGACAATCCGGGAAGTACCGTGATCACGGTGTGAGAACCCTTGTATGGCACCCTC
TTCAATGTCGTGGAAACGGAGACGTTGGCATTGTTCGAGCATCTCTCCTTCGAGTTTCTTGAAGAGTTCGACGTGTTCGCCCCGGCGAAGATGGGGCGAA
CACGAGATCTTGAGCCGCCCGAGATGATGCGTGGCTTTCTCCATTGCTATTACAAGAACATCTACGGCATCCGTCCGGTTGCACGGAAACTGAACAACAC
CGTTGTCTGGCTCAGCTGTGGCTTCGATCGACCGCTGTCAAGAGATGCGGTCGATCGCTTTCTCACTGATCTTGAGCACGTCGTCGACCGGGTCTTCGAC
CATCTCGTCGAGCAGGTCGCCTTGCGGGGCCTGCTCGAGTTAACGTATTCTATCGATTCAACCGATGTGAGAGCAATGCCCGCCGATCCAGACGCATCGA
AGTGCTCTGATCCAACCGATGACGAGTACTACTACGGCTACGGCTACGGCTGCACGATCGTCTCGACCGGGCAAAAGATCCCGATTGCAGCCGAGTTCAC
CGAGAGCAAACAAGCACCAGAAGAGACGGCGATGCGCGTCACGCGTGACGCACTCGCCGTCGAGAAACCGATCTGGATGCTTGGAGACAGCGCTTACGAC
ACGCTCGAGTGGCACGACCACCTGCTGACCGCAGGGGTCGTGCCAGTCGCTCCGTACAATCCACGAAACACCGACGACCCGAAAAACATCGAGTACGGGG
TAGAAGACCGCATCGAAAAACACAGCAATGACGTTCAGCTGAAGCAATCAACGCTAGACGAGACGTACAACCGCCGGAGTGGCGTCGAACGAACCAACGA
ATCAGTCAAGGGCTGCGGTCTCGGGCGAACGCACGCCCGAGGCCGCGTCCATTCACGAGCGTAGGTGTTCCTCGCGTTGTGTCTGCGCCTCGTCGTCGCA
ATCACCAACTACGAACGAGGAGACAATCCGGGAAGTACCGTGATCACGGTGTGAGAACCCTTGTATGGCACCCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 999 bp | 332 aa | 56 | 1054 | + | No |
Chemistry : DDE
ORF sequence :
MSSATLQDDPSVESFFNVVETETLALFEHLSFEFLEEFDVFAPAKMGRTRDLEPPEMMRGFLHCYYKNIYGIRPVARKLNNTVVWLSCGFDRPLSRDAVD
RFLTDLEHVVDRVFDHLVEQVALRGLLELTYSIDSTDVRAMPADPDASKCSDPTDDEYYYGYGYGCTIVSTGQKIPIAAEFTESKQAPEETAMRVTRDAL
AVEKPIWMLGDSAYDTLEWHDHLLTAGVVPVAPYNPRNTDDPKNIEYGVEDRIEKHSNDVQLKQSTLDETYNRRSGVERTNESVKGCGLGRTHARGRVHS
RA*VFLALCLRLVVAITNYERGDNPGSTVITV
RFLTDLEHVVDRVFDHLVEQVALRGLLELTYSIDSTDVRAMPADPDASKCSDPTDDEYYYGYGYGCTIVSTGQKIPIAAEFTESKQAPEETAMRVTRDAL
AVEKPIWMLGDSAYDTLEWHDHLLTAGVVPVAPYNPRNTDDPKNIEYGVEDRIEKHSNDVQLKQSTLDETYNRRSGVERTNESVKGCGLGRTHARGRVHS
RA*VFLALCLRLVVAITNYERGDNPGSTVITV
Blast result :
Comments
ISHwa20 is 93% aa similar to ISH11.
In strain C23, the transposase gene contains an in-frame stop codon; the element has been lost from strain HBSQ001.
Additional copies of the element are even further disrupted (frameshifts, targetting by other transposons).
The target duplication of this copy of the element shows a one-base mutation.
In strain C23, the transposase gene contains an in-frame stop codon; the element has been lost from strain HBSQ001.
Additional copies of the element are even further disrupted (frameshifts, targetting by other transposons).
The target duplication of this copy of the element shows a one-base mutation.
References
1] Dyall-Smith,M.L., Pfeiffer,F., Klee,K., Palm,P., Gross,K., Schuster,S.C., Rampp, M., and Oesterhelt,D. (2011) PLoS One 6: e20968