ISAtc2

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010600 ND Acidithiobacillus caldus
Acidithiobacillus caldus plasmid pTcM1
DNA section
IS Length : 1304 bp

Ends


IR Length : 20/25

IRL : GGCTCTTCGACATTTTGAGTGGGTAAGGCGGTATGATTGCCTAGTGGAGC
IRR : GGTTCTTCGACGTTTCATGTGGGTAGCCGGAGATCGAGCTTACCCAGGAT

Insertion site


Left flankDirect repeatRight flankDR Length
GGAACCTGGACACAATTCTC0

DNA sequence

GGCTCTTCGACATTTTGAGTGGGTAAGGCGGTATGATTGCCTAGTGGAGCGGTCAGCAAGCCGAGGGGATTTATGGACCAAGGGAATCAGCTGTTCACGC
TGGCATTGGGCCTAGTTCCACCGTGGGCGGTGGACGTGGTTCGGTTTACGGTGGAGGAGAAGCGCCTGGACCTCTATGTGAACTTCTCAAGAGGTAGCCA
CTTCCCTTGTCCGGTCTGCGGTCAGGATTGTCCCGTCCATGACACGCAGGAGAAGGTCTGGCGCCACCTCGACTTCTTCCAGCATGCGGCCTACCTCCAT
GCTCGGGTGCCGCGGGTCCAGTGCCCGGAACACGGTGTGCATTTGGTGCCCGTCCCCTGGGCGCGGGAAGGCTCAGGATTCACACTGCTCTTCGAGGCTC
TCGTCATGGCCATGGTCCGGGAGATGCCGGTGTTGACGGTTTCCCGATTGGTCCGGGAGACGGACCAGCGACTCTGGCGGGTGATCGACCATTATGTCTC
CAAAGCCCGCGAGGCGGTGGACATGTCGGAGGTTCAGGCCATCGGTGTCGACGAAACCAGCAGCCGGCGCGGCCACGACTACATCTCCCTGTTCGTGGAC
CTGGCGGCCAAGCGCTTACTCTTCGCCACCCCAGGCAAGGATGCTCAGACCTTTGAGAAGTTCTGCGAAGATCTGCAGGCCCATGGTGGTAACTCCGAGG
CTATTGCGGAGGTGAGCATGGACCTCTCTCCAGCCTTCCAAAAAGGTGCCGCAGAACACCTACCCAACGCCCAGGTCACCTTCGATCGCTTTCACCTGAT
GAAGCTCATCAATGAGGCCGTGGACGTCGTGCGCAAGGGAGAGGTGTACTCCCAACCCGACCTAAAGAAGAGCCGCTGGCTTTGGCTCAAGAATCCAGGA
AAGCTCTCGGCCAAGCAGAGCGCCAAGCTCCAGGAGCTCCTCAAGAACCAGAACCTCAAGACGGCCCAGGCCTACCAGTTCCGCCTGACCTTCCAAGAAA
TCTTCACGATCCAGAATCGCCACCAGGGCGCTGTGCTCCTCAAGGCCTGGATGGAAAACGCCAAGGCCAGCGGACTACCCCCTCTGGTCAAAGTCACCTA
CACCCTCATGAACCACTGGGATGGCATCCTCCGCTGGTTCGAGAGCCAGATCACCAACGGTATCTTGGAGGGCTTCAACAGCCTCCTGCAGTCTGCCAAG
GCCAAGGCCCGTGGCTACCGCACCCACAAGAACTTCATCAATATGGCCTATCTCATCCTGGGTAAGCTCGATCTCCGGCTACCCACATGAAACGTCGAAG
AACC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1218 bp405 aa731290+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MDQGNQLFTLALGLVPPWAVDVVRFTVEEKRLDLYVNFSRGSHFPCPVCGQDCPVHDTQEKVWRHLDFFQHAAYLHARVPRVQCPEHGVHLVPVPWAREG
SGFTLLFEALVMAMVREMPVLTVSRLVRETDQRLWRVIDHYVSKAREAVDMSEVQAIGVDETSSRRGHDYISLFVDLAAKRLLFATPGKDAQTFEKFCED
LQAHGGNSEAIAEVSMDLSPAFQKGAAEHLPNAQVTFDRFHLMKLINEAVDVVRKGEVYSQPDLKKSRWLWLKNPGKLSAKQSAKLQELLKNQNLKTAQA
YQFRLTFQEIFTIQNRHQGAVLLKAWMENAKASGLPPLVKVTYTLMNHWDGILRWFESQITNGILEGFNSLLQSAKAKARGYRTHKNFINMAYLILGKLD
LRLPT

 

Blast result :
Comments
ISAtc2 is 90% aa similar to ISTfe1.
References
1] ISfinder annotation (2010)
2] van Zyl,L.J., Louw,L.-A., Deane,S.M. and Rawlings,D.E.(2008) Direct submission GenBank.