ISfinder Mirror Site

ISHut1

Family IS5
Group ISH1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013158	ND	Halorhabdus utahensis	Halorhabdus utahensis DSM 12940

DNA section

IS Length : 926 bp

Ends

IR Length : 20/22

IRL : GGCTCTGTAGTTTCGCTAGACGCGACAGCAGCGACTGCGTTATTGCCGTA
IRR : GGCTCTGTAGTTTCGGTACACGGCGGTTGATTTCACGGATATCTCACGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CATAATTACT	CTCATTTT	GGACTAACAG	8

DNA sequence

GGCTCTGTAGTTTCGCTAGACGCGACAGCAGCGACTGCGTTATTGCCGTAGAAAGCGAAGAGACGGAGTATTGTGACTTCGGAATTCCGTCTCTTCACCC
GTGTTACGGTCGCTAAGGCTAAATCAGTTGTCGCTAACCCGGACGAACCCGCCGACCCCGAAGGGGGTGGCGGGTTCGCCGAGTGGGCGATGCTCACGCT
GCATGCACTCCGCATCGAGCTGGGCAAATCCTACCGCGCCGCGGTGGATTTGCTCAGCGAGATGCCAGGCGTCCTCGAAGAAATCGGCCTCACGCGACTG
CCTCACTACACGGTTCTCCGCACGTGGTTTGCCCGGATTCCGACTACAACGTGGCGTGCGTTTCTCGGCGCGTCAGCCGAGAAACGCACTGGCCACGCTG
CTATCGATTCGACTGGCTTCGACCGCGATCAGCCGAGCCGTCACTACGCCAACCGCACGAACTACCGCGTTCGAGCGCTGAAAGTCACTGCTCTCGTGGA
TGTCGAAACGCTGTACATCACCGACATCCACTCGACGACCTCGAAGAAACACGATGCGAAGATCGGCCCGCAGGTCGCCCGGCGCAACGCCGGCGACCTG
CGGAGCCTCGCTGCTGACCGAGGCTATGACGCGAAAACCTTCCGCGACGAACTCCGTGAGAACGGTATCAGACCGCTGATCAAGCACAGGATCATGAACC
CGCTCGATCACGCGCATAACGCCCGCATGGACGGTGATCGGTATCATCAGCGCTCCATGTCAGAAACCGTCTTCTCCTCGATCAAGCGCACGCTCGGCGC
TGCCGTGCGTGCGCGCAGCTGGTGGCTGGAATTCCGTGAAATGCTGTTGAAAGCGACCGTCTACAACCTTCGCCGAAGCGTGAGATATCCGTGAAATCAA
CCGCCGTGTACCGAAACTACAGAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
822 bp	273 aa	73	894	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTSEFRLFTRVTVAKAKSVVANPDEPADPEGGGGFAEWAMLTLHALRIELGKSYRAAVDLLSEMPGVLEEIGLTRLPHYTVLRTWFARIPTTTWRAFLGA
SAEKRTGHAAIDSTGFDRDQPSRHYANRTNYRVRALKVTALVDVETLYITDIHSTTSKKHDAKIGPQVARRNAGDLRSLAADRGYDAKTFRDELRENGIR
PLIKHRIMNPLDHAHNARMDGDRYHQRSMSETVFSSIKRTLGAAVRARSWWLEFREMLLKATVYNLRRSVRYP

Blast result :

Comments

ISHut1 is 73% aa similar to ISHwa1.

References

1] ISfinder annotation (2010).

2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Mikhailova,N., Ovchinnikova,G., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Tindal,B., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.