ISAcp2
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012483 | ND | Acidobacterium capsulatum | Acidobacterium capsulatum ATCC 51196 |
DNA section
IS Length : 1025 bp
Ends
IR Length : 19/27
IRL : TGTGATGTTTCAAGAGGTTGTCCATTCGAAATTTCGCGGAGAAGCTGTTT
IRR : TGTAGAAGGTCAAGAGGTTGTTCCTTCTTGGGATCGGCTGCTTGGCGGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGACACCGG | GGCACC | CGGCAATCAT | 6 |
| AGAGGCTTGAGTGGGGCACC | CCCACCCGCCAAAGAAGGAA | 0 | |
| ACCGGAATGG | GGCACC | CGACCGTGCC | 6 |
| GAATCGAGACGTGGGGCACC | GCCACCCAAAATGCTTCGCT | 0 | |
| ACGGACGTGG | GGCACC | CGAGTTGGGATGAG | 6 |
| CGAGATGTGG | GGCACC | CGGCCTTCAC | 6 |
| ATGGACGTGG | GGCACC | CGGGATGGTT | 6 |
| GCTTGAGTGG | GCCACC | CGATGCATAG | 6 |
| GCTTGAGTGG | GGCACC | CGAGATGTGG | 6 |
| ACGGACGTGG | GGCACC | CGGTTTGTTT | 6 |
| TGAGATGTGG | GGCACC | CGGTTCGCTT | 6 |
DNA sequence
TGTGATGTTTCAAGAGGTTGTCCATTCGAAATTTCGCGGAGAAGCTGTTTGTGGCGAACAAGCAGAGTCTTCGGAGAGATCGAATGGACATTCACCAGAA
TGCTCGTCTAACGCCTTACAGTCGAGAGCAGTTGGCGAGAAAAGTTATTTGTACCGGGTGCACGTTGAAGCTGGCCGCGGCCAGCTTCAACGTGAGCGCA
AAGACGGCCGGCAAATGGGTGCGCCGGTATCGCGCCGAGGGTTCGGATGGCTTGCGGGACCGCAGCTCGCGTCCGCATCGCAGCCCGCGGCGCTTGCCGG
AGGCGTTGCGGCTGAGTGTGATTGAGCTACGGCGGGGTTACATGCCGGGGTATCAGATCGCCCGGCGCAGCGCTGTGAGCGTGTCTTCGGTGAGCCGTAT
TTTGCGGCGCGCGCGGCTGAGCCGATGGCGCGATCTGAACCCGCCTCCGCCGGTGGTTCGCTATGAGCATGCCGCTCCAGGCGACTTGCTGCATCTGGAC
ATCAAAGGCATGACGCGCTTCGGCGAGGTCTCGCTGCGCGGCGACGGCAGGCTGCGGGGCAAGAAGGAACACCCGGGCTTTTTGGCTCTGCATGTGGCCG
TCGACGATCACTCGCGCATGGTCTTCGCCCAGATGCTGGCCGATCAGAAGGCGGAAACCACGATCGGTTTTCTGCACGCGGCGGTTGAGTTTTTCGCCAG
CCATGGTATCGGCATCCGCGCGCTGCTGACCGACAACGGCAGCAGCTACCGCTCTCGCCAGTTCCGTCAGGCTTGCCAGCAGATGGCCATCAAACACAGC
CGCACTCGGCCCTATACCCCCAGAACCAACGGCAAGGCCGAGCGCTTCATCCAGACAGCCATGCGCGAATGGGCTTACGCCAAACACTGGACCGACTCCA
GCCAGAGAGATCAACACTTGCAGTCCTGGATCCACTACTACAACCACGAAAGACCTCATGGTAGCCTCAACTACAAACCGCCAAGCAGCCGATCCCAAGA
AGGAACAACCTCTTGACCTTCTACA
TGCTCGTCTAACGCCTTACAGTCGAGAGCAGTTGGCGAGAAAAGTTATTTGTACCGGGTGCACGTTGAAGCTGGCCGCGGCCAGCTTCAACGTGAGCGCA
AAGACGGCCGGCAAATGGGTGCGCCGGTATCGCGCCGAGGGTTCGGATGGCTTGCGGGACCGCAGCTCGCGTCCGCATCGCAGCCCGCGGCGCTTGCCGG
AGGCGTTGCGGCTGAGTGTGATTGAGCTACGGCGGGGTTACATGCCGGGGTATCAGATCGCCCGGCGCAGCGCTGTGAGCGTGTCTTCGGTGAGCCGTAT
TTTGCGGCGCGCGCGGCTGAGCCGATGGCGCGATCTGAACCCGCCTCCGCCGGTGGTTCGCTATGAGCATGCCGCTCCAGGCGACTTGCTGCATCTGGAC
ATCAAAGGCATGACGCGCTTCGGCGAGGTCTCGCTGCGCGGCGACGGCAGGCTGCGGGGCAAGAAGGAACACCCGGGCTTTTTGGCTCTGCATGTGGCCG
TCGACGATCACTCGCGCATGGTCTTCGCCCAGATGCTGGCCGATCAGAAGGCGGAAACCACGATCGGTTTTCTGCACGCGGCGGTTGAGTTTTTCGCCAG
CCATGGTATCGGCATCCGCGCGCTGCTGACCGACAACGGCAGCAGCTACCGCTCTCGCCAGTTCCGTCAGGCTTGCCAGCAGATGGCCATCAAACACAGC
CGCACTCGGCCCTATACCCCCAGAACCAACGGCAAGGCCGAGCGCTTCATCCAGACAGCCATGCGCGAATGGGCTTACGCCAAACACTGGACCGACTCCA
GCCAGAGAGATCAACACTTGCAGTCCTGGATCCACTACTACAACCACGAAAGACCTCATGGTAGCCTCAACTACAAACCGCCAAGCAGCCGATCCCAAGA
AGGAACAACCTCTTGACCTTCTACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 933 bp | 310 aa | 84 | 1016 | + | No |
Chemistry : DDE
ORF sequence :
MDIHQNARLTPYSREQLARKVICTGCTLKLAAASFNVSAKTAGKWVRRYRAEGSDGLRDRSSRPHRSPRRLPEALRLSVIELRRGYMPGYQIARRSAVSV
SSVSRILRRARLSRWRDLNPPPPVVRYEHAAPGDLLHLDIKGMTRFGEVSLRGDGRLRGKKEHPGFLALHVAVDDHSRMVFAQMLADQKAETTIGFLHAA
VEFFASHGIGIRALLTDNGSSYRSRQFRQACQQMAIKHSRTRPYTPRTNGKAERFIQTAMREWAYAKHWTDSSQRDQHLQSWIHYYNHERPHGSLNYKPP
SSRSQEGTTS
SSVSRILRRARLSRWRDLNPPPPVVRYEHAAPGDLLHLDIKGMTRFGEVSLRGDGRLRGKKEHPGFLALHVAVDDHSRMVFAQMLADQKAETTIGFLHAA
VEFFASHGIGIRALLTDNGSSYRSRQFRQACQQMAIKHSRTRPYTPRTNGKAERFIQTAMREWAYAKHWTDSSQRDQHLQSWIHYYNHERPHGSLNYKPP
SSRSQEGTTS
Blast result :
Comments
ISAcp2 is 66% aa similar to ISRel2.
There is a conflict with the name of this IS, we have renamed it because it used a name (ISAca1) already attributed to another sequence in Acinetobacter calcoaceticus.
There is a conflict with the name of this IS, we have renamed it because it used a name (ISAca1) already attributed to another sequence in Acinetobacter calcoaceticus.
References
1] ISfinder annotation (2010)
2] Ward,N.L., Challacombe,J.F., Janssen,P.H., Henrissat,B., Coutinho,P.M., Wu,M., Xie,G., Haft,D.H., Sait,M., Badger,J., Barabote,R.D., Bradley,B., Brettin,T.S., Brinkac,L.M., Bruce,D., Creasy,T., Daugherty,S.C., Davidsen,T.M., DeBoy,R.T., Detter,J.C., Dodson,R.J., Durkin,A.S., Ganapathy,A., Gwinn-Giglio,M., Han,C.S., Khouri,H., Kiss,H., Kothari,S.P., Madupu,R., Nelson,K.E., Nelson,W.C., Paulsen,I., Penn,K., Ren,Q., Rosovitz,M.J., Selengut,J.D., Shrivastava,S., Sullivan,S.A., Tapia,R., Thompson,L.S., Watkins,K.L., Yang,Q., Yu,C., Zafar,N., Zhou,L. and Kuske,C.R. (2009) Appl. Environ. Microbiol. 75 (7), 2046-2056.
2] Ward,N.L., Challacombe,J.F., Janssen,P.H., Henrissat,B., Coutinho,P.M., Wu,M., Xie,G., Haft,D.H., Sait,M., Badger,J., Barabote,R.D., Bradley,B., Brettin,T.S., Brinkac,L.M., Bruce,D., Creasy,T., Daugherty,S.C., Davidsen,T.M., DeBoy,R.T., Detter,J.C., Dodson,R.J., Durkin,A.S., Ganapathy,A., Gwinn-Giglio,M., Han,C.S., Khouri,H., Kiss,H., Kothari,S.P., Madupu,R., Nelson,K.E., Nelson,W.C., Paulsen,I., Penn,K., Ren,Q., Rosovitz,M.J., Selengut,J.D., Shrivastava,S., Sullivan,S.A., Tapia,R., Thompson,L.S., Watkins,K.L., Yang,Q., Yu,C., Zafar,N., Zhou,L. and Kuske,C.R. (2009) Appl. Environ. Microbiol. 75 (7), 2046-2056.