ISfinder Mirror Site

ISApr13

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Alpha proteobacterium	Alpha proteobacterium BAL199

DNA section

IS Length : 1556 bp

Ends

IR Length : 17

IRL : CAGGCTGTTGAAGAAGTCGATGGAGCGGTAGCTGGTGGCATGATTCACTG
IRR : CAGGCTGTTGAAGAAGTGACGCGTTCGGCTTTGAGGGTAGCTTCGTCGCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCGGAGCGCC	CTAG	AATTTCTTCG	4

DNA sequence

CAGGCTGTTGAAGAAGTCGATGGAGCGGTAGCTGGTGGCATGATTCACTGGATCGGGTAGATGGTCGAGGTGGATCGATGCGCGTCGGTGATGACGGTTC
AGGAGAGTTGTTCAGCTACGTCGACCTTGAGGCTCGGGTTCGCCGGGACCACCCGCTGCGGGCGATCCGGGAGATTGCGAACGCGGCTCTGGATACGCTG
AGCGAGGACTTTGCGGCGCTGTACTGTCGGATGGGCCGTCCGTCGATCGCACCAGAGCGGCTGCTTCGGGCGATGCTTCTGCAGGCGTTCTACTCGATCC
GCTCGGAACGCCAGCTGATGGAGCGGCTGGAATACGACCTTCTGTTCCGCTGGTTCGTCGGGCTCGGTCTGGACGACGCAGTGTGGGACCATTCGACGTT
CTCGAAGAACCGCGACCGGTTGCTGGCGGGAGAGATCGCCGGTCGGTTCCTGGCGGCGGTGCTGGCTCAGCCGCGGGTGAAGCGGCTGCTGTCCAGCGAG
CACTTCTCGGTCGACGGCACGCTGATCGAGGCGTGGGCCAGCTTGAAGAGCTTCAAGCCGAAGGACCCCGGCGCCCCTCCATCCGCGGGCGATGGCAACG
GCGGAGACCGCAACGCATCGGTCGACTTCAAGGGCCAGAAGCGCTCAAACGCGACGCACGCCTCGACGACCGATCCCGATGCGCTGCTGTACCGCAAGGG
ACCGGGGATGGAGGCACGACTGTGCTACATCGGCCACGGGCTCATGGAGAACCGTTCGGGCCTGATCGTCGACACCCGGCTGACCCGGGTATCGGGCCAC
GCCGAGCGGCTGGCGGCGCTGGAGATGATCGAGCCCTGGGGCGACCGTTCGAGTGCCGTCACCCTCGGCGCCGACAAGGGCTACGATGCCCGCGACTTCG
TGATGGAACTGCGCGAGGTGAACGTGCGCCCACATCTGGCGCAGAACACCAGCAACCGGCGTTCCGCGATCGACCGCCGCACCACCCGCCATCCGGGCTA
TGCGGTGAGCCAGCGCATCCGCAAGCGGATCGAAGGTGAAGCGAGACCGTGAGCGAAGCTTGCGTAAGCTCGCTTCACGGCGGCTGGATCAAGACCGTGG
CCGGGATGCGCAAGACCCGGTTCCGCGGCCGGGAGCGCGTCGACTTGGCCTTCACCTTCGCGGCCGCCGCCTACAACTTGGTGCGGCTGCCCAAGTTGCT
GGCCGAGGTGCAGTCATGACCGCGCCGATGGGCTGCCAGTTGATCGGCCGGTGGCGGATCGTTGAAGCCGACATCTGGGATCGCGACTACCTTGACCTGG
TCGAGCCGGCGAAGATGGTCATCGAGGCTGGCGGACACGGCGAGATCGCCTTCGGGGCCATGCAGGCCGGGCTCGACATCGAATACGCCCGCACCCTGGT
GTTCTTCACCTGGGAGGGCTTCGACGAGATGGACCACGTCCGCGGCACCGGATCCGCCGAACTCCAGGACGACGGCACCATCGAGATCGAGTTCGCCTAC
CACCTGGGCGACGAAGCTACCCTCAAAGCCGAACGCGTCACTTCTTCAACAGCCTG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1142 bp	380 aa	78	1219	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRVGDDGSGELFSYVDLEARVRRDHPLRAIREIANAALDTLSEDFAALYCRMGRPSIAPERLLRAMLLQAFYSIRSERQLMERLEYDLLFRWFVGLGLDD
AVWDHSTFSKNRDRLLAGEIAGRFLAAVLAQPRVKRLLSSEHFSVDGTLIEAWASLKSFKPKDPGAPPSAGDGNGGDRNASVDFKGQKRSNATHASTTDP
DALLYRKGPGMEARLCYIGHGLMENRSGLIVDTRLTRVSGHAERLAALEMIEPWGDRSSAVTLGADKGYDARDFVMELREVNVRPHLAQNTSNRRSAIDR
RTTRHPGYAVSQRIRKRIEGEARPMSEACVSSLHGGWIKTVAGMRKTRFRGRERVDLAFTFAAAAYNLVRLPKLLAEVQS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
345 bp	114 aa	1216	1560	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTAPMGCQLIGRWRIVEADIWDRDYLDLVEPAKMVIEAGGHGEIAFGAMQAGLDIEYARTLVFFTWEGFDEMDHVRGTGSAELQDDGTIEIEFAYHLGDE
ATLKAERVTSSTAC

Blast result :

Comments

ISApr13 is 90% aa similar to ISApr12. The transposase was reconstructed in silico, there is a premature STOP codon and a frameshift.
The second ORF is a passenger gene annotated as hypothetical protein.
Genome in progress : accession number : NZ_ABHC01000010.

References

1] ISfinder annotation (2010)
2] Hagstrom,A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.