ISfinder Mirror Site

ISApr12

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_ABHC01000046	ND	Alpha proteobacterium	Alpha proteobacterium BAL199

DNA section

IS Length : 1524 bp

Ends

IR Length : 17

IRL : CAGGCTGTTGAAGAAGTCGGTGGAGGGTCCGCTGGCGACGTAATTCACTC
IRR : CAGGCTGTTGAAGAAGTGACGCGTTCGGCTTTGAGGGTAGCCTCGTCGCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTTCTCGCGG	ATAG	TGGTGAGCGA	4

DNA sequence

CAGGCTGTTGAAGAAGTCGGTGGAGGGTCCGCTGGCGACGTAATTCACTCGATTGGTGAGGTGGTCGAGGTGGATCGATGCGTGGCGGTGACCGTCAACC
GGGTGAGTTGTTCAGCTACATCGACCTTGAGGCTCGGGTGCGTCGGGACCATCCGCTGCGGGCGATCCGGGAGATCGCGAACGCCGCGCTGGAGACGCTG
AGCGGCGACTTTGCGGCGCTGTACTCGCGGATGGGCCGCCCGTCGATCGCGCCGGAGCGGCTGCTTCGGGCGATGCTGTTGCAGGCGTTCTATTCGATCC
GCTCGGAGCGCCAGCTGATGGAGAGGCTGGAGTACGACCTGCTGTTCCGCTGGTTCGTCGGGCTCGGCCTCGACGATGCGGTGTGGGACCACTCGACGTT
CTCGAAGAACCGCGATCGGCTGCTGGCGGGAGAGATCGCCGGGCGGTTCCTGGATGCGGTGCTGGCGCAGCCGCGGGTCAAGCGGCTGCTGTCCAGCGAT
CACTTCTCGGTCGACGGCACGCTGATCCAGGCCTGGGCCAGCCTGAAGAGCTTCAAGCCGAAGGAGGGCGCGCAGCCGCCGTTGGACGGCGACGATGCTG
GCGCCGGCGGCGGGCGCAATTCCGAAGTCGACTTCAAGGGCCAGAAGCGCTCGAACGCGACGCATGCCTCGACGACCGACCCGGATGCGCTGCTGTACCG
CAAGGGGCCGGGGATGGAGGCACGGCTGTGCTACATCGGGCACGGGCTGATGGAGAACCGCTCGGGCCTGATCGTCGACACCAGGCTCACGAGGGTGTCC
GGCCATGCCGAGCGGCTGGCGGCTTTGGAGATGATCGAGCCTTGGGGCGAGCGGAGCCGCGCCATCACCCTCGGCGCCGACAAGGGCTACGACACCCGCG
ACTTCGTTATGGAGTTGCGCGAACTCAACGTGCGTCCGCACCTGGCGCGGAACACCAGTGGCAGGCGGTCGGCGATCGACCGACGCACCACCCGTCACCC
CGGCTATGCCACCAGCCAGCGCGTCCGCAAGCGGATCGAGGAGGCATTCGGCTGGATTAAGACGGTTGCTGGGCTCCGCCAGACGAGGTTCCGCGGTCGG
GAACGGGTCGACCTGGCGTTCACCTTCGCGGCCGCCGCCTACAACCTGGTCCGGCTGCCCAGGCTGCTGGCGGAGGCGCCGCCGTGAGCACACCGATGGA
CTGCCAGTTGATCGGCCGCTGGCGGATCGTCAAGGCTGACATATGGGATCGCGACTACCTGGACCTGGTCGAGCCCGCCATGTTGGTCATCCAGGCCAAC
GGCCACGGCGAGATCGCCTACGGCGCCATGCAGGCGAGCCTCGACCTCGAATACAGCCGAGCGCTGGTGTACTTCACCTGGGAGGGCTTCGACGAGATGG
ACGAGGTGCGCGGCTCCGGCTCCGCCGAACTCCAGGACGACGGCACCCTCGAGATCGAGTTCGCCTACCGCCACGGCGACGAGGCTACCCTCAAAGCCGA
ACGCGTCACTTCTTCAACAGCCTG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1110 bp	369 aa	78	1187	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGGDRQPGELFSYIDLEARVRRDHPLRAIREIANAALETLSGDFAALYSRMGRPSIAPERLLRAMLLQAFYSIRSERQLMERLEYDLLFRWFVGLGLDD
AVWDHSTFSKNRDRLLAGEIAGRFLDAVLAQPRVKRLLSSDHFSVDGTLIQAWASLKSFKPKEGAQPPLDGDDAGAGGGRNSEVDFKGQKRSNATHASTT
DPDALLYRKGPGMEARLCYIGHGLMENRSGLIVDTRLTRVSGHAERLAALEMIEPWGERSRAITLGADKGYDTRDFVMELRELNVRPHLARNTSGRRSAI
DRRTTRHPGYATSQRVRKRIEEAFGWIKTVAGLRQTRFRGRERVDLAFTFAAAAYNLVRLPRLLAEAPP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
330 bp	109 aa	1196	1525	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDCQLIGRWRIVKADIWDRDYLDLVEPAMLVIQANGHGEIAYGAMQASLDLEYSRALVYFTWEGFDEMDEVRGSGSAELQDDGTLEIEFAYRHGDEATLK
AERVTSSTA

Blast result :

Comments

ISApr12 (ORF1, the transposase) is 82% aa similar to ISNisp7. The second ORF is a passenger gene, annotated as hypothetical protein, its STOP codon is in the DR.

References

1] ISfinder annotation (2010)
2] Hagstrom,A., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.