ISAtc5

  • Family IS5
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_ACVD01000027 ND Acidithiobacillus caldus
Acidithiobacillus caldus ATCC 51756
DNA section
IS Length : 1260 bp

Ends


IR Length : 15/18

IRL : GAGGCTGCTGAAAAAGTCGGAGGCATAATGGGCAGAACGAGGCCGGAGAG
IRR : GAGGGTGCGGAAAAAATCCGTAGAAACGCCGTGGCGCTATCCGATTTGGT

Insertion site


Left flankDirect repeatRight flankDR Length
TGCGAGGGGGGTAGAGGTGAGCGA4

DNA sequence

GAGGCTGCTGAAAAAGTCGGAGGCATAATGGGCAGAACGAGGCCGGAGAGATTGGAACGATGCGTGGAGCCAAGGTAGAGACCCAAGGGTTATTCAGCTA
CGTTTCCCCGGAGCAACGGGTGCCCCAGGACCATCCGCTGAGAGCGATCCGGGCTATCGTGGATCGCTCCCTGGCGGAGATGGACAGCCACTTCAGCACG
ATGTACTCGAGCTACGGTCGCCCCTCCATTCCCCCGGAGTTTCTCCTGCGCGCACTGCTCCTGCAGGTCTTCTACAGCATCCGCTCGGAACGGCAGCTGA
TGGAGCAACTCAACTACAACCTGCTGTTCCGCTGGTTTGTCGGTTTGGGAATGGACGACGAGGTCTGGGTGCCCACGGTCTTTACTAAGAATCGCGATCG
GTTGCTGGATCACGGGACGGTACGGGAATTCTTCCGATCCGTACTGGAACAGGCTCGCGGCCAGAATCTGCTCTCCGAAGAGCATTTCTCCGTCGATGGT
ACGCTGTTGGAGGCCTGGGCCTCGCAGAAATCCTTTCAGCCCAAGGATCCCGAAGATCGTAGCGGTGATGGCTCCGACTTCCGGGGCCAGGAGCGCAGCA
ATGAGACCCACGCATCGGTGACGGACCCCGATGCCCGGCTGTACAAGAAGGCTCCCGGAGAGGCGTCCCGTCTGGCCTACCTGGGTCATGTGCTAATGGA
TAATCGGCATGGATTGATTGCCGGGGAACAGGTGACCACCGCCGATGGCACGGCAGAAGTGGATGCCGCTACCCAATTGGTGGACGATCTGGGCGGGAAC
CAGCGCATCACCCTCGGCGCAGATAAGGGTTATGACCGCCACGGCTTCGTCCAGAATCTCCGGGATCGGAACGTGACCCCACATGTAGCGCGCAAGCGCA
AAGGCTCGGCCATCGACGGGCGCACCACCCGCCACGCCGGTTACGCGATGAGCATCCACGTTCGGCGGCGGATCGAGTCCATCTTCGGCTGGATGAAGAC
GGTGGGAGGGATGCGGAAAACCCGATTCCGTGGTTTGGAACGGGTCGGTCTGCACTTTTCCTTGGTCACTGTCGCCTACAACCTGGTCCGCATGGTGCGG
TTGGGGGTGGCCTGATGGGGGTACTGCGTCTGGAATCCGAAAATGGCCCAAAAAAGACTGCAACAGCGGACAAAACAGGACTGATAGGGCTGCCTATTCG
GCAATTTTTGACCAAATCGGATAGCGCCACGGCGTTTCTACGGATTTTTTCCGCACCCTC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1056 bp351 aa601115+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGAKVETQGLFSYVSPEQRVPQDHPLRAIRAIVDRSLAEMDSHFSTMYSSYGRPSIPPEFLLRALLLQVFYSIRSERQLMEQLNYNLLFRWFVGLGMDD
EVWVPTVFTKNRDRLLDHGTVREFFRSVLEQARGQNLLSEEHFSVDGTLLEAWASQKSFQPKDPEDRSGDGSDFRGQERSNETHASVTDPDARLYKKAPG
EASRLAYLGHVLMDNRHGLIAGEQVTTADGTAEVDAATQLVDDLGGNQRITLGADKGYDRHGFVQNLRDRNVTPHVARKRKGSAIDGRTTRHAGYAMSIH
VRRRIESIFGWMKTVGGMRKTRFRGLERVGLHFSLVTVAYNLVRMVRLGVA

 

Blast result :
Comments
ISAtc5 is 69% aa similar to ISGsu2.
References
1] Isfinder annotation (2010)
2] Valdes,J., Quatrini,R., Hallberg,K., Mangold,S., Dopson,M., Valenzuela,P.D. and Holmes,D.S. (2009 ) J. Bacteriol. In press