ISfinder Mirror Site

ISRosp6

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_ABCR01000002	ND	Roseobacter sp.	Roseobacter sp. AzwK-3b

DNA section

IS Length : 1357 bp

Ends

IR Length : 17/18

IRL : CAGGCTGCTGAAATAGTCCTGCCTCGACAACGATTTGAGAACATGATTCA
IRR : CAGTCTGCTGAAATAGTCGCGGAGCTGGAACGCTTTCCCTTCATCAGGTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCTTGTGCCG	TTAG	AGGTCTCTGC	4
TCAGTCCCCA	TTAA	AGCTTTTGCG	4
TCAGTCCCCC	TTAG	GGTCCCCTTC	4

DNA sequence

CAGGCTGCTGAAATAGTCCTGCCTCGACAACGATTTGAGAACATGATTCACCCTTTGCACGATAATGGCGGGGGTGAAGATGCGCGGGACGGACGAGACG
AGCGGGTCACTGTTCAGCTATGTTGATCTTGAGGAGCGCATCCCGCCGCAGCATCCGCTGCGCAAGATCAGGCAGGTGGTCAATGACGCACTCGCCAGCC
TCGATGCGGAGTTTGAGGCGCTTTACACAGATTTTGGCCGACCCTCGATACCGCCGGAACGGTTGATCCGGGCGAGCCTGCTGCAAATCCTGTTTTCGGT
CCGGTCCGAGCGGCAACTGATGGAGCAGATGGATTACAACCTGATGTTTCGCTGGTTTGTGGGGCTGGGCATTGATGACCCCGTGTGGGTGCCAACCGTA
TTCACGAAGAATCGCGACCGGCTGCTGACGACGGAGATGTCGCGCAAGGTGATGGCGGCGATCCTTGCCCATCGCGAGATCGCGCCACTTCTGTCGGACG
AACACTTCTCGGTCGATGGCACGCTGGTGAAAGCCTGGGCCTCAATGAAGAGTTTCCAGCCGAAGGCCGATGCGGCCCCGCCTTATGATGAAGGGCCGGG
CGATCCGCCCTCCCCAAATGCCACACTTGAGCCCACGCCTTCTGAGACCGACCCAATGCCCCGCAACACCAAACCCCACCGCAATGCCGAGGTCGACTTC
AAGGGTGAGAAGCGATCCAACGCCACCCATGCCTCAACCACTGATCCCGATGCGCGCCTTTACAAGAAATCCCCTGGTACGGGTGCCATGCTGTGCTTCA
TGGGGCATACTCTGATGGAGAACCGTAACGGGCTGGTCGTGCAGGGTGATCTAACCTTGGCCGACGGTCATGCCGAGCGCAAGGCAGCGTTGGATATGAT
ACACCGCCATTGCCCCGGATCGACCCGGCGGCTGACGCTAGGTGCCGACAAGGGGTATGACGCGGCCGGGTTCGTGTCCGACCTGCGCAAAGCCTGCGTC
ACGCCCCACGTCGCACAGAAATCGCGATATTCAGCGATCGACGGGCGCACGACCCGACACGAGGGATATGCGGTGTCCCAGAAGCACCGAAAGAAGATCG
AAGAGCCGTTCGGCTGGGCCAAAACTGTCGGCGGTATGACACAGACCATGTATCGCGGCGTTGAACGCGTCCGATCCCGCTTCATCTTGACCATGGCCGC
CAACAATCTTGCCCGGCTACCACGTTTGCTGGGGGCATGATGGATAAGATGAAACCCAGGAGCAAGCCGCGAGGGCAACAACTGACGGGAACCATCAGTC
GAACCCAGACCTGATGAAGGGAAAGCGTTCCAGCTCCGCGACTATTTCAGCAGACTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1176 bp	391 aa	65	1240	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAGVKMRGTDETSGSLFSYVDLEERIPPQHPLRKIRQVVNDALASLDAEFEALYTDFGRPSIPPERLIRASLLQILFSVRSERQLMEQMDYNLMFRWFVG
LGIDDPVWVPTVFTKNRDRLLTTEMSRKVMAAILAHREIAPLLSDEHFSVDGTLVKAWASMKSFQPKADAAPPYDEGPGDPPSPNATLEPTPSETDPMPR
NTKPHRNAEVDFKGEKRSNATHASTTDPDARLYKKSPGTGAMLCFMGHTLMENRNGLVVQGDLTLADGHAERKAALDMIHRHCPGSTRRLTLGADKGYDA
AGFVSDLRKACVTPHVAQKSRYSAIDGRTTRHEGYAVSQKHRKKIEEPFGWAKTVGGMTQTMYRGVERVRSRFILTMAANNLARLPRLLGA

Blast result :

Comments

ISRosp6 is 66% aa similar to ISMex28.

References

1] ISfinder annotation (2010)
2] Francis,C., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.