ISEcB1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012967 | ND | Escherichia coli | Escherichia coli B REL606 Escherichia coli BL21(DE3) |
DNA section
IS Length : 1441 bp
Ends
IR Length : 19/24
IRL : TGAACTGCACCCCAAAAGTTGGACACTCAACGAAGTAAGGTGCAGTTTTT
IRR : TGAACTGACCCCATAATGTTGGACGGTTTATATTAAGCGGCTATCAGGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AACCGCCGAG | CATG | TCGGCGGCTT | 4 |
| CCGCCGAATG | AATTTGGCGG | 0 | |
| AACCGCCGAG | CATG | TCGGCGGCTT | 4 |
| AACCGCCGAG | CATG | TCGGCGGCTT | 4 |
| CCGCCGAATG | AATTTGGCGG | 0 | |
| AACCGCCGAG | CATG | TCGGCGGCTT | 4 |
DNA sequence
TGAACTGCACCCCAAAAGTTGGACACTCAACGAAGTAAGGTGCAGTTTTTTATGAGTAAATATTCTCTCAGCTTTAAGCTTGAGGTTGTGCAGCACTATC
TTTCCGGCATGGAAGGGCAGAAAGCGACTGCAAAGCGTTTTGGTATCGATCACAGTGCCGTTCGTAAGTGGACTGCTGCCTGGAAGCTTCATGGCGAAGC
CGGACTTACCACCCGGCATTTCGCCTATTCTCCCGTCTTTAAAGAGTCCGTTATCCTGCATATGCGTGAGCACCGGTTGTCTGTTCGCGAAGTCTGTGCA
AAATTCACTATTCCGGCCTTCTCCACGGTCCTTCTGTGGGAACGCCTGTATGATGAAGGGGGATTAGAAGCCCTCACTGACAGCCGACGCGGGAGAAAAA
CGATGCCAGAACGCCCTGAAAATAACCCGGCACCTTCTTTACAGGCTCCTCTTAACCATGATGAACGAGAGGAACTTGAACAACTGCGTGTTGAAGTGGC
TTATCTAAAAAAGTTGCAGGCCTTGCTCAGGGAAAAAAGCACGTCAAAACGCGGGATAAAACGCAGATCGTGAATGAGTTAAGGCCGGACTTCCCTCTTG
CGCGCTTGTTAAAAGCGGCAGGGCTGGCGAGAAGTACGTTCTACTGGCAACTGGCGAGCGGTAAAAAACCAGACAAATATGCCGATGATAAGCGGCAGAT
TTTAACGCTGTTCCATAAACATAAAGGACGGTATGGATACAGGCGAATCACGCTGGCTATCCGCCGGGAAGGAAGCTTACTGAACCATAAAAAGGTTCAG
AGGTTAATGAAGGCGTTAGATTTAACCTGCTGTATCAGAAGGAAGAAATACAACTCTTACAGAGGCCGGTATGGGAAAGTGGCGGGTAATGTGCTGAACC
GGCAGTTCAGTGCGGATAAGCCCAACCAGAAGTGGGTAACAGATGTCACGGAATTTAAAATCAGCGGAAAAAAACTGTATCTGTCCCCCGTTCTGGATCT
GTATAACGGAGAAATAGTTGCGTATCAGATGGCGTCCCGTCCTCTTCTGTCGATGGTGGATGACATGCTGTTGAAGGCTGTATCTGTACTGGGTAAAGAC
GAATACCCGCTGCTCCACTCAGATCAGGGCTGGCAATATCAGATGGCGCATTATCAGAACCGGCTTAAGGAGGCTGGTCTGAAACAGAGCATGTCAAGGA
AGGGGAACTGTCATGACAATGCGGTGATGGAAAGCTTCTTCGGCACGCTGAAATCTGAGTGTTTTTACCTGATGGAACACAAGAATATCAGCGAGTTGCG
CATGGCTATCGATAATTACATCCACTATTACAACCATGAAAGAATAAAACAGAAACTGAAAGGCCTGAGTCCGGTAGAGTACCGAACCCAGGCCCTGATA
GCCGCTTAATATAAACCGTCCAACATTATGGGGTCAGTTCA
TTTCCGGCATGGAAGGGCAGAAAGCGACTGCAAAGCGTTTTGGTATCGATCACAGTGCCGTTCGTAAGTGGACTGCTGCCTGGAAGCTTCATGGCGAAGC
CGGACTTACCACCCGGCATTTCGCCTATTCTCCCGTCTTTAAAGAGTCCGTTATCCTGCATATGCGTGAGCACCGGTTGTCTGTTCGCGAAGTCTGTGCA
AAATTCACTATTCCGGCCTTCTCCACGGTCCTTCTGTGGGAACGCCTGTATGATGAAGGGGGATTAGAAGCCCTCACTGACAGCCGACGCGGGAGAAAAA
CGATGCCAGAACGCCCTGAAAATAACCCGGCACCTTCTTTACAGGCTCCTCTTAACCATGATGAACGAGAGGAACTTGAACAACTGCGTGTTGAAGTGGC
TTATCTAAAAAAGTTGCAGGCCTTGCTCAGGGAAAAAAGCACGTCAAAACGCGGGATAAAACGCAGATCGTGAATGAGTTAAGGCCGGACTTCCCTCTTG
CGCGCTTGTTAAAAGCGGCAGGGCTGGCGAGAAGTACGTTCTACTGGCAACTGGCGAGCGGTAAAAAACCAGACAAATATGCCGATGATAAGCGGCAGAT
TTTAACGCTGTTCCATAAACATAAAGGACGGTATGGATACAGGCGAATCACGCTGGCTATCCGCCGGGAAGGAAGCTTACTGAACCATAAAAAGGTTCAG
AGGTTAATGAAGGCGTTAGATTTAACCTGCTGTATCAGAAGGAAGAAATACAACTCTTACAGAGGCCGGTATGGGAAAGTGGCGGGTAATGTGCTGAACC
GGCAGTTCAGTGCGGATAAGCCCAACCAGAAGTGGGTAACAGATGTCACGGAATTTAAAATCAGCGGAAAAAAACTGTATCTGTCCCCCGTTCTGGATCT
GTATAACGGAGAAATAGTTGCGTATCAGATGGCGTCCCGTCCTCTTCTGTCGATGGTGGATGACATGCTGTTGAAGGCTGTATCTGTACTGGGTAAAGAC
GAATACCCGCTGCTCCACTCAGATCAGGGCTGGCAATATCAGATGGCGCATTATCAGAACCGGCTTAAGGAGGCTGGTCTGAAACAGAGCATGTCAAGGA
AGGGGAACTGTCATGACAATGCGGTGATGGAAAGCTTCTTCGGCACGCTGAAATCTGAGTGTTTTTACCTGATGGAACACAAGAATATCAGCGAGTTGCG
CATGGCTATCGATAATTACATCCACTATTACAACCATGAAAGAATAAAACAGAAACTGAAAGGCCTGAGTCCGGTAGAGTACCGAACCCAGGCCCTGATA
GCCGCTTAATATAAACCGTCCAACATTATGGGGTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 522 bp | 173 aa | 52 | 573 | + | No |
Description : First part of the transposase
ORF sequence :
MSKYSLSFKLEVVQHYLSGMEGQKATAKRFGIDHSAVRKWTAAWKLHGEAGLTTRHFAYSPVFKESVILHMREHRLSVREVCAKFTIPAFSTVLLWERLY
DEGGLEALTDSRRGRKTMPERPENNPAPSLQAPLNHDEREELEQLRVEVAYLKKLQALLREKSTSKRGIKRRS
DEGGLEALTDSRRGRKTMPERPENNPAPSLQAPLNHDEREELEQLRVEVAYLKKLQALLREKSTSKRGIKRRS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 495 | 1409 | + | No |
Description : Second part of the transposase
ORF sequence :
SGLSKKVAGLAQGKKHVKTRDKTQIVNELRPDFPLARLLKAAGLARSTFYWQLASGKKPDKYADDKRQILTLFHKHKGRYGYRRITLAIRREGSLLNHKK
VQRLMKALDLTCCIRRKKYNSYRGRYGKVAGNVLNRQFSADKPNQKWVTDVTEFKISGKKLYLSPVLDLYNGEIVAYQMASRPLLSMVDDMLLKAVSVLG
KDEYPLLHSDQGWQYQMAHYQNRLKEAGLKQSMSRKGNCHDNAVMESFFGTLKSECFYLMEHKNISELRMAIDNYIHYYNHERIKQKLKGLSPVEYRTQA
LIAA
VQRLMKALDLTCCIRRKKYNSYRGRYGKVAGNVLNRQFSADKPNQKWVTDVTEFKISGKKLYLSPVLDLYNGEIVAYQMASRPLLSMVDDMLLKAVSVLG
KDEYPLLHSDQGWQYQMAHYQNRLKEAGLKQSMSRKGNCHDNAVMESFFGTLKSECFYLMEHKNISELRMAIDNYIHYYNHERIKQKLKGLSPVEYRTQA
LIAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1358 bp | 452 aa | 52 | 1409 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKYSLSFKLEVVQHYLSGMEGQKATAKRFGIDHSAVRKWTAAWKLHGEAGLTTRHFAYSPVFKESVILHMREHRLSVREVCAKFTIPAFSTVLLWERLY
DEGGLEALTDSRRGRKTMPERPENNPAPSLQAPLNHDEREELEQLRVEVAYLKKVAGLAQGKKHVKTRDKTQIVNELRPDFPLARLLKAAGLARSTFYWQ
LASGKKPDKYADDKRQILTLFHKHKGRYGYRRITLAIRREGSLLNHKKVQRLMKALDLTCCIRRKKYNSYRGRYGKVAGNVLNRQFSADKPNQKWVTDVT
EFKISGKKLYLSPVLDLYNGEIVAYQMASRPLLSMVDDMLLKAVSVLGKDEYPLLHSDQGWQYQMAHYQNRLKEAGLKQSMSRKGNCHDNAVMESFFGTL
KSECFYLMEHKNISELRMAIDNYIHYYNHERIKQKLKGLSPVEYRTQALIAA
DEGGLEALTDSRRGRKTMPERPENNPAPSLQAPLNHDEREELEQLRVEVAYLKKVAGLAQGKKHVKTRDKTQIVNELRPDFPLARLLKAAGLARSTFYWQ
LASGKKPDKYADDKRQILTLFHKHKGRYGYRRITLAIRREGSLLNHKKVQRLMKALDLTCCIRRKKYNSYRGRYGKVAGNVLNRQFSADKPNQKWVTDVT
EFKISGKKLYLSPVLDLYNGEIVAYQMASRPLLSMVDDMLLKAVSVLGKDEYPLLHSDQGWQYQMAHYQNRLKEAGLKQSMSRKGNCHDNAVMESFFGTL
KSECFYLMEHKNISELRMAIDNYIHYYNHERIKQKLKGLSPVEYRTQALIAA
Blast result :
Comments
ISEcB1 is 68% aa similar to ISKpn1. The third ORF is a putative oRFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Jeong,H., Barbe,V., Lee,C.H., Vallenet,D., Yu,D.S., Choi,S.H., Couloux,A., Lee,S.W., Yoon,S.H., Cattolico,L., Hur,C.G., Park,H.S., Segurens,B., Kim,S.C., Oh,T.K., Lenski,R.E., Studier,F.W., Daegelen,P. and Kim,J.F. (2009) J. Mol. Biol. 394 (4), 644-652