ISRm17
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003047 | ND | Sinorhizobium meliloti | Sinorhizobium meliloti 1021
Sinorhizobium meliloti 1021 plasmid pSymA |
DNA section
IS Length : 1664 bp
Ends
IR Length : 21/27
IRL : GTGCCCATCCATAAACAGCAAGTATTTGGAATTATTGCGTAAAACGCGCT
IRR : GTGCCCGTCCATAAACGCCTGTTTTTCAGGGTTTTAGAAGTGTTTTGCTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACTTGTCCTC | TAGCTA | GGGATGACAC | 6 |
| AACTGACGGT | TAGCTA | GCCGGTCACA | 6 |
| TCCTCCAGGC | TAGCTA | GGCGAGATGT | 6 |
| AGCAATTCCC | TAGACTA | GGCACGCCGA | 7 |
| TCCCTCAGGC | TAGTCTA | GCGGTCGCCG | 7 |
DNA sequence
GTGCCCATCCATAAACAGCAAGTATTTGGAATTATTGCGTAAAACGCGCTTTTTGTGCGGGCAAGGCCCGGCGGTTTTAGGTACACTTTAGCATCAAGCC
ACTGATTCTAAAGACAAACCCGCAACCGCCGGAGCCGACAATGCGCCAAGAACGCACCGTCCAGGGCAGCATATTCGATCTTTTCGCCGAGCACGAGATC
GGTCGTGAGTTGGAGGCGATGTCGCAGTGGCTGGACGCCCATCGCGATCTTTTGAATTTGGTGACGTCCGATCTGCGCCGACAGGGGGTCACGGAAACTG
GCCGGCAGGGTTTGCCGTCGGAGGCGGTGCTGCGCTGTGCCCTCCTCAAACAGTACCGCCAGTTGAGCTACGAGGAGTTGGCGTTTCATCTGGAAGATTC
CGCCTCGTTCCGGGCCTTTGCCCGGCTGCCATGGGGATGGAGCCCGAAAAAGTCGGTCTTGCACAAGACGATCAGCGCGATCCGGGCGGACACCTGGGAA
GCGGTCAACAAAATGCTGCTGGCCAGCGCCCGGCAAGAAAGGTTGGAAAGCGGCAGGGTCGTGCGTGTGGACAGCACCGTCACGGCGGCGCTGATCCACG
AACCAAGCGACAGCAGTCTTTTGTGGGACTGCGTGCGGGTGATGGTTCGTCTTCTGCAGCAGGCGGATTCGCTGGGCAGCACCATTCCCTGGCACGATCA
CTGCCGCGCGGCGAAGAAGCGGGCTCGGGTGATCGAGTATACCCGCGGTCGCCCGAAACGGGTTCAGCACTACCGCGCTCTGCTCAGGATCGCCCGGAAC
ACCCTGGATTATCTGCAGCAGGCGGCGGCACAGTTGCCGCTGGCGGCGGGCCCGGCGGGCAAACTCTGGCAGGCCCAGGTTCGCCACTATCAGCCACTGA
TCACGCAGATCATCGCCCAGACCGAGCGGCGGGTGCTAGCCGGCGAAGCAGTGCCGGCCGGCGAGAAGCTTGTCAGCCTGTTCGAACCGCATGCCGACAT
CATCGTCAAGGGCAGCCGCGACGTCGATTACGGACATAAGCTCAATCTGACCACCGGCAGGAGCGGACTGATCCTCGATCTCGTCATCGAAGCCGGCAAT
CCAGCCGACAGCGAGCGGCTCTTGCCCCTACTGGAGCGCCACATCGCCTTCTATGGCGAGGCGCCGCGGCAGGCGGCCGCCGACGGCGGCTATGCAAGCC
GTGAAAACCTGCGCCAGGCCAAGGCCTGGGGTGTACGCGACATGGCCTTCCATAAGAAGAGCGGCCTCAGGATCGAAGACATGGTCAGGAGCCGCTGGGT
GTATCGAAAGCTCAGAAACTTCCGGGCCGGCATCGAGGCCGGCATCTCCTGCCTGAAGCGGACCTATGGCCTGGCCCGCTGCACCTGGCGCGGGCTTGAC
CACTTCAAGACCTACGTCTGGTCCTCGGTGGTTGCCTACAATCTCGCCCTCTTCGCCCGTCTCAGGCCGACCTAACGCCGGTCATCGCGCCGCGTTCCGG
AACACGCCGCGAGCGCCGGCCGATGCCACACGACCCTATCGCCGCCAAATCGCCCTTCGATCACAACCTGCAAGCCCTGTGCCATCACGCCTCGCGAATA
AAGACCGCTTCAGCGAGCAAAACACTTCTAAAACCCTGAAAAACAGGCGTTTATGGACGGGCAC
ACTGATTCTAAAGACAAACCCGCAACCGCCGGAGCCGACAATGCGCCAAGAACGCACCGTCCAGGGCAGCATATTCGATCTTTTCGCCGAGCACGAGATC
GGTCGTGAGTTGGAGGCGATGTCGCAGTGGCTGGACGCCCATCGCGATCTTTTGAATTTGGTGACGTCCGATCTGCGCCGACAGGGGGTCACGGAAACTG
GCCGGCAGGGTTTGCCGTCGGAGGCGGTGCTGCGCTGTGCCCTCCTCAAACAGTACCGCCAGTTGAGCTACGAGGAGTTGGCGTTTCATCTGGAAGATTC
CGCCTCGTTCCGGGCCTTTGCCCGGCTGCCATGGGGATGGAGCCCGAAAAAGTCGGTCTTGCACAAGACGATCAGCGCGATCCGGGCGGACACCTGGGAA
GCGGTCAACAAAATGCTGCTGGCCAGCGCCCGGCAAGAAAGGTTGGAAAGCGGCAGGGTCGTGCGTGTGGACAGCACCGTCACGGCGGCGCTGATCCACG
AACCAAGCGACAGCAGTCTTTTGTGGGACTGCGTGCGGGTGATGGTTCGTCTTCTGCAGCAGGCGGATTCGCTGGGCAGCACCATTCCCTGGCACGATCA
CTGCCGCGCGGCGAAGAAGCGGGCTCGGGTGATCGAGTATACCCGCGGTCGCCCGAAACGGGTTCAGCACTACCGCGCTCTGCTCAGGATCGCCCGGAAC
ACCCTGGATTATCTGCAGCAGGCGGCGGCACAGTTGCCGCTGGCGGCGGGCCCGGCGGGCAAACTCTGGCAGGCCCAGGTTCGCCACTATCAGCCACTGA
TCACGCAGATCATCGCCCAGACCGAGCGGCGGGTGCTAGCCGGCGAAGCAGTGCCGGCCGGCGAGAAGCTTGTCAGCCTGTTCGAACCGCATGCCGACAT
CATCGTCAAGGGCAGCCGCGACGTCGATTACGGACATAAGCTCAATCTGACCACCGGCAGGAGCGGACTGATCCTCGATCTCGTCATCGAAGCCGGCAAT
CCAGCCGACAGCGAGCGGCTCTTGCCCCTACTGGAGCGCCACATCGCCTTCTATGGCGAGGCGCCGCGGCAGGCGGCCGCCGACGGCGGCTATGCAAGCC
GTGAAAACCTGCGCCAGGCCAAGGCCTGGGGTGTACGCGACATGGCCTTCCATAAGAAGAGCGGCCTCAGGATCGAAGACATGGTCAGGAGCCGCTGGGT
GTATCGAAAGCTCAGAAACTTCCGGGCCGGCATCGAGGCCGGCATCTCCTGCCTGAAGCGGACCTATGGCCTGGCCCGCTGCACCTGGCGCGGGCTTGAC
CACTTCAAGACCTACGTCTGGTCCTCGGTGGTTGCCTACAATCTCGCCCTCTTCGCCCGTCTCAGGCCGACCTAACGCCGGTCATCGCGCCGCGTTCCGG
AACACGCCGCGAGCGCCGGCCGATGCCACACGACCCTATCGCCGCCAAATCGCCCTTCGATCACAACCTGCAAGCCCTGTGCCATCACGCCTCGCGAATA
AAGACCGCTTCAGCGAGCAAAACACTTCTAAAACCCTGAAAAACAGGCGTTTATGGACGGGCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 141 | 1475 | + | No |
Description :
ORF sequence :
MRQERTVQGSIFDLFAEHEIGRELEAMSQWLDAHRDLLNLVTSDLRRQGVTETGRQGLPSEAVLRCALLKQYRQLSYEELAFHLEDSASFRAFARLPWGW
SPKKSVLHKTISAIRADTWEAVNKMLLASARQERLESGRVVRVDSTVTAALIHEPSDSSLLWDCVRVMVRLLQQADSLGSTIPWHDHCRAAKKRARVIEY
TRGRPKRVQHYRALLRIARNTLDYLQQAAAQLPLAAGPAGKLWQAQVRHYQPLITQIIAQTERRVLAGEAVPAGEKLVSLFEPHADIIVKGSRDVDYGHK
LNLTTGRSGLILDLVIEAGNPADSERLLPLLERHIAFYGEAPRQAAADGGYASRENLRQAKAWGVRDMAFHKKSGLRIEDMVRSRWVYRKLRNFRAGIEA
GISCLKRTYGLARCTWRGLDHFKTYVWSSVVAYNLALFARLRPT
SPKKSVLHKTISAIRADTWEAVNKMLLASARQERLESGRVVRVDSTVTAALIHEPSDSSLLWDCVRVMVRLLQQADSLGSTIPWHDHCRAAKKRARVIEY
TRGRPKRVQHYRALLRIARNTLDYLQQAAAQLPLAAGPAGKLWQAQVRHYQPLITQIIAQTERRVLAGEAVPAGEKLVSLFEPHADIIVKGSRDVDYGHK
LNLTTGRSGLILDLVIEAGNPADSERLLPLLERHIAFYGEAPRQAAADGGYASRENLRQAKAWGVRDMAFHKKSGLRIEDMVRSRWVYRKLRNFRAGIEA
GISCLKRTYGLARCTWRGLDHFKTYVWSSVVAYNLALFARLRPT
Blast result :
Comments
ISRm17 is 62% aa similar to ISDol1.
There is 3 copies of ISRm17 in Sinorhizobium meliloti 1021 chromosome and 2 copies in Sinorhizobium meliloti 1021 plasmid pSymA.
There is 3 copies of ISRm17 in Sinorhizobium meliloti 1021 chromosome and 2 copies in Sinorhizobium meliloti 1021 plasmid pSymA.
References
1]Capela,D., Barloy-Hubler,F., Gouzy,J., Bothe,G., Ampe,F., Batut,J., Boistard,P., Becker,A., Boutry,M., Cadieu,E., Dreano,S., Gloux,S., Godrie,T., Goffeau,A., Kahn,D., Kiss,E., Lelaure,V., Masuy,D., Pohl,T., Portetelle,D., Puehler,A., Purnelle,B., Ramsperger,U., Renard,C., Thebault,P., Vandenbol,M., Weidner,S. and Galibert,F. (2001) Proc. Natl. Acad. Sci. U.S.A. 98 (17), 9877-9882
2]Galibert,F., Finan,T.M., Long,S.R., Puhler,A., Abola,P., Ampe,F., Barloy-Hubler,F., Barnett,M.J., Becker,A., Boistard,P., Bothe,G., Boutry,M., Bowser,L., Buhrmester,J., Cadieu,E., Capela,D., Chain,P., Cowie,A., Davis,R.W., Dreano,S., Federspiel,N.A., Fisher,R.F., Gloux,S., Godrie,T., Goffeau,A., Golding,B., Gouzy,J., Gurjal,M., Hernandez-Lucas,I., Hong,A., Huizar,L., Hyman,R.W.,Jones,T., Kahn,D., Kahn,M.L., Kalman,S., Keating,D.H., Kiss,E., Komp,C., Lelaure,V., Masuy,D., Palm,C., Peck,M.C., Pohl,T.M., Portetelle,D., Purnelle,B., Ramsperger,U., Surzycki,R., Thebault,P., Vandenbol,M., Vorholter,F.J., Weidner,S., Wells,D.H., Wong,K., Yeh,K.C. and Batut,J. (2001) Science 293 (5530), 668-672.
3]ISfinder annotation (2008).
2]Galibert,F., Finan,T.M., Long,S.R., Puhler,A., Abola,P., Ampe,F., Barloy-Hubler,F., Barnett,M.J., Becker,A., Boistard,P., Bothe,G., Boutry,M., Bowser,L., Buhrmester,J., Cadieu,E., Capela,D., Chain,P., Cowie,A., Davis,R.W., Dreano,S., Federspiel,N.A., Fisher,R.F., Gloux,S., Godrie,T., Goffeau,A., Golding,B., Gouzy,J., Gurjal,M., Hernandez-Lucas,I., Hong,A., Huizar,L., Hyman,R.W.,Jones,T., Kahn,D., Kahn,M.L., Kalman,S., Keating,D.H., Kiss,E., Komp,C., Lelaure,V., Masuy,D., Palm,C., Peck,M.C., Pohl,T.M., Portetelle,D., Purnelle,B., Ramsperger,U., Surzycki,R., Thebault,P., Vandenbol,M., Vorholter,F.J., Weidner,S., Wells,D.H., Wong,K., Yeh,K.C. and Batut,J. (2001) Science 293 (5530), 668-672.
3]ISfinder annotation (2008).