ISRsp17
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_012587 | ND | Rhizobium sp. | Rhizobium sp. NGR234 Rhizobium sp. NGR234 plasmid pNGR234b |
DNA section
IS Length : 1664 bp
Ends
IR Length : 15/17
IRL : GTGCCCGTCCACAAACGGTAAACTGCTGAAATTATTGGGTGAATCGCGAT
IRR : GTGCCCATCCATAAACGCCTGTTTTTCTGCGGTTTTGCAAGGTTGTTTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGAGATCGGC | TAGACTA | GAAGCGACGT | 7 |
| GCATCCGCTC | TAGTCTA | GCGGCACCCCGCA | 7 |
| CAAATCGAAC | TAGCTA | GTTCCATGCGAACG | 6 |
| TGGAAATGGC | TAGACTA | GCGAAGAACA | 7 |
DNA sequence
GTGCCCGTCCACAAACGGTAAACTGCTGAAATTATTGGGTGAATCGCGATATCTGCGCGGGCAAAGCCCGGCGGTTTCAGGTATACTTTAGATCAAGCTA
CTGATTCTAAAGACAAACCCGTAACCGCCGGAGCCGACAATGCGCCAAGAACGCACCGTCCAAGCCAGCATATTTGATCTTTTCGCCGAACACGAGATCG
GTCGTGAACTGAAGGCGATGTCGGAATGGCTGGATGAGCATGGCGAGTTGCTCGGGCTGGTGGCGGGGGACCTGCGCCGGCATGGTCTCAGACAGACCGG
CCGCGAGGGGCTACCAGCCGAGGCTGTGCTGCGCTGCGCGCTGCTCAAGCAGCATCGCCAGTTGAGTTACCAGGAACTGGCCTTCCATCTCGAAGACTCC
GCCTCGTTCCGGGCGTTTGCCCGGCTGCCGTGGGGCTGGAACCCGAAGAAGTCGGTCTTGCACAAGACGATCAGCGCGATCCGGGCGCAAACCTTTGAAG
AGATCAACGGGGTGCTGCTGGCGAGCGCCCGGCAGGAGAAGCTGGAAAGTGGCAAGGTCGTGCGCGTGGACAGCACCGTCACCGCCGCACCGATACATGA
ACCGAGCGACAGCAGCCTGTTATGGGACGCTGTCCGGGTGACGGTGCGGCTGTTGCAGCAGGCCGAGGCGCTGGGTAGCGCCATCCCATGGCACGACCAT
CGCCGCGCGGCAAAGAAGCGGGCTCGGGCGATCGAATATACCCGCGGCCGCCCCAAACGAATCAGGCACTATCGCGAACTGCTCAAGATCACGCACACGA
CTTTGAGTTATCTGCAGCAGGCCAGTGAACAGGTGCCGCTGACGGCGGGCCCGGCCGGCGCACTGTGGCAGGCCCAACTCCGCCATTACCGGCCGCTGAT
CGAGCGGATCATCGCCCAGACCGAACAGCGGGTCCTGGTCGGGGAGCCGGTCCCTGCCGGCGAGAAGCTGGTGAGCCTGTTCGAGCCGCATGCCGACATC
ATCGTCAAGGGCAGCCGCGACGTCGACTACGGCCACAAACTCAATCTGACCACCGGCAGAAGCGGACTGATCCTCGACCTCGTCATCGAAGCCGGCAATC
CGACCGACAGCGAGCGCTTGCTGCCGATGCTGGAACGCCACATCGCCTTTTACGGCGAGCCGCCGCGTCAGGCGGCGGCCGACGGCGGCTATGCCAGCCG
CGAAAACCTGAGCGGAGCCAAAGCCCGCGGCATCCGCGACATGGCCTTCCACAAGAAGCGCGGCCTCAGCATCGAAGACATGGTCAGCAGCCGCTGGGTC
TATCGCAAGCTGCGCAACTTCCGCGCCGGCATCGAGGCCGGCATCTCTTGCCTGAAACGCGCTTATGGCTTGGGGCGCTGCACCTGGCGTGGGCTCGACC
ACTTCAAGGCTTATGTCTGGTCCTCGGTGGTCGCTTACAATCTCGCCCTCTTCGCCCGCCTCAGGCCGACCTGACGCTCCTGATCCGGTGGCGTTCAGAG
CAAGGCCGGCGAGCGTCGGCCGATACCCCATGACCCATTGCCGCCAGATCGCTCTTCGGGCCATAACCTGCGCCGCCTTGTGCCATCACGCGTCCCAAGA
AACGATGTCAGCCGGCAAACAACCTTGCAAAACCGCAGAAAAACAGGCGTTTATGGATGGGCAC
CTGATTCTAAAGACAAACCCGTAACCGCCGGAGCCGACAATGCGCCAAGAACGCACCGTCCAAGCCAGCATATTTGATCTTTTCGCCGAACACGAGATCG
GTCGTGAACTGAAGGCGATGTCGGAATGGCTGGATGAGCATGGCGAGTTGCTCGGGCTGGTGGCGGGGGACCTGCGCCGGCATGGTCTCAGACAGACCGG
CCGCGAGGGGCTACCAGCCGAGGCTGTGCTGCGCTGCGCGCTGCTCAAGCAGCATCGCCAGTTGAGTTACCAGGAACTGGCCTTCCATCTCGAAGACTCC
GCCTCGTTCCGGGCGTTTGCCCGGCTGCCGTGGGGCTGGAACCCGAAGAAGTCGGTCTTGCACAAGACGATCAGCGCGATCCGGGCGCAAACCTTTGAAG
AGATCAACGGGGTGCTGCTGGCGAGCGCCCGGCAGGAGAAGCTGGAAAGTGGCAAGGTCGTGCGCGTGGACAGCACCGTCACCGCCGCACCGATACATGA
ACCGAGCGACAGCAGCCTGTTATGGGACGCTGTCCGGGTGACGGTGCGGCTGTTGCAGCAGGCCGAGGCGCTGGGTAGCGCCATCCCATGGCACGACCAT
CGCCGCGCGGCAAAGAAGCGGGCTCGGGCGATCGAATATACCCGCGGCCGCCCCAAACGAATCAGGCACTATCGCGAACTGCTCAAGATCACGCACACGA
CTTTGAGTTATCTGCAGCAGGCCAGTGAACAGGTGCCGCTGACGGCGGGCCCGGCCGGCGCACTGTGGCAGGCCCAACTCCGCCATTACCGGCCGCTGAT
CGAGCGGATCATCGCCCAGACCGAACAGCGGGTCCTGGTCGGGGAGCCGGTCCCTGCCGGCGAGAAGCTGGTGAGCCTGTTCGAGCCGCATGCCGACATC
ATCGTCAAGGGCAGCCGCGACGTCGACTACGGCCACAAACTCAATCTGACCACCGGCAGAAGCGGACTGATCCTCGACCTCGTCATCGAAGCCGGCAATC
CGACCGACAGCGAGCGCTTGCTGCCGATGCTGGAACGCCACATCGCCTTTTACGGCGAGCCGCCGCGTCAGGCGGCGGCCGACGGCGGCTATGCCAGCCG
CGAAAACCTGAGCGGAGCCAAAGCCCGCGGCATCCGCGACATGGCCTTCCACAAGAAGCGCGGCCTCAGCATCGAAGACATGGTCAGCAGCCGCTGGGTC
TATCGCAAGCTGCGCAACTTCCGCGCCGGCATCGAGGCCGGCATCTCTTGCCTGAAACGCGCTTATGGCTTGGGGCGCTGCACCTGGCGTGGGCTCGACC
ACTTCAAGGCTTATGTCTGGTCCTCGGTGGTCGCTTACAATCTCGCCCTCTTCGCCCGCCTCAGGCCGACCTGACGCTCCTGATCCGGTGGCGTTCAGAG
CAAGGCCGGCGAGCGTCGGCCGATACCCCATGACCCATTGCCGCCAGATCGCTCTTCGGGCCATAACCTGCGCCGCCTTGTGCCATCACGCGTCCCAAGA
AACGATGTCAGCCGGCAAACAACCTTGCAAAACCGCAGAAAAACAGGCGTTTATGGATGGGCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 140 | 1474 | + | No |
Chemistry : DDE
ORF sequence :
MRQERTVQASIFDLFAEHEIGRELKAMSEWLDEHGELLGLVAGDLRRHGLRQTGREGLPAEAVLRCALLKQHRQLSYQELAFHLEDSASFRAFARLPWGW
NPKKSVLHKTISAIRAQTFEEINGVLLASARQEKLESGKVVRVDSTVTAAPIHEPSDSSLLWDAVRVTVRLLQQAEALGSAIPWHDHRRAAKKRARAIEY
TRGRPKRIRHYRELLKITHTTLSYLQQASEQVPLTAGPAGALWQAQLRHYRPLIERIIAQTEQRVLVGEPVPAGEKLVSLFEPHADIIVKGSRDVDYGHK
LNLTTGRSGLILDLVIEAGNPTDSERLLPMLERHIAFYGEPPRQAAADGGYASRENLSGAKARGIRDMAFHKKRGLSIEDMVSSRWVYRKLRNFRAGIEA
GISCLKRAYGLGRCTWRGLDHFKAYVWSSVVAYNLALFARLRPT
NPKKSVLHKTISAIRAQTFEEINGVLLASARQEKLESGKVVRVDSTVTAAPIHEPSDSSLLWDAVRVTVRLLQQAEALGSAIPWHDHRRAAKKRARAIEY
TRGRPKRIRHYRELLKITHTTLSYLQQASEQVPLTAGPAGALWQAQLRHYRPLIERIIAQTEQRVLVGEPVPAGEKLVSLFEPHADIIVKGSRDVDYGHK
LNLTTGRSGLILDLVIEAGNPTDSERLLPMLERHIAFYGEPPRQAAADGGYASRENLSGAKARGIRDMAFHKKRGLSIEDMVSSRWVYRKLRNFRAGIEA
GISCLKRAYGLGRCTWRGLDHFKAYVWSSVVAYNLALFARLRPT
Blast result :
Comments
ISRsp17 is 95% aa similar to ISRle10.
References
1] ISfinder annotation (2010).
2] Schmeisser,C., Liesegang,H., Krysciak,D., Bakkou,N., Le Quere,A., Wollherr,A., Heinemeyer,I., Morgenstern,B., Pommerening-Roser,A., Flores,M., Palacios,R., Brenner,S., Gottschalk,G., Schmitz,R.A., Broughton,W.J., Perret,X., Strittmatter,A.W. and Streit,W.R. (2009) Appl. Environ. Microbiol. 75 (12), 4035-4045.
3] Liesegang,H., Krysziak,D., Schmeisser,C., Bakkou,N., Le Quere,A., Strittmatter,A.W., Wollherr,A., Heinemeyer,I., Morgenstern,B., Gottschalk,G., Schmitz,R.A., Broughton,W.J., Perret,X. and Streit,W.R. (2007) Direct submission GenBank.
2] Schmeisser,C., Liesegang,H., Krysciak,D., Bakkou,N., Le Quere,A., Wollherr,A., Heinemeyer,I., Morgenstern,B., Pommerening-Roser,A., Flores,M., Palacios,R., Brenner,S., Gottschalk,G., Schmitz,R.A., Broughton,W.J., Perret,X., Strittmatter,A.W. and Streit,W.R. (2009) Appl. Environ. Microbiol. 75 (12), 4035-4045.
3] Liesegang,H., Krysziak,D., Schmeisser,C., Bakkou,N., Le Quere,A., Strittmatter,A.W., Wollherr,A., Heinemeyer,I., Morgenstern,B., Gottschalk,G., Schmitz,R.A., Broughton,W.J., Perret,X. and Streit,W.R. (2007) Direct submission GenBank.