ISRjo3
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008269 | ND | Rhodococcus jostii | Rhodococcus jostii RHA1 plasmid pRHL1 Rhodococcus jostii RHA1 plasmid pRHL2 |
DNA section
IS Length : 1638 bp
Ends
IR Length : 15
IRL : CTTCGACCTGAAAAAGTCGGGGTGTGGCGGTACGTGATCAGCCAGTCCTG
IRR : CTTCGACCTGAAAAACGAGGCGCCCCGGTGAGCATGATTCGGCCGTTCTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCGGCCGAA | TAGCTA | GTCTCCGGAC | 6 |
| AATGCAATCC | TAGCTA | GTGGTGTGTC | 6 |
| CAGCCCTCCT | TAGCTA | AGCTAAGTGG | 6 |
DNA sequence
CTTCGACCTGAAAAAGTCGGGGTGTGGCGGTACGTGATCAGCCAGTCCTGAACCGGAACAATGGCCTCAACCCGTTGGGCTAGAAGGGATCGAGGCCATC
GTGTTCCGTACCGTAGGCGATCAACCGTCGTTGTTCGAGTCGGTGCTGCCGCAGGAGTTGCTGCGGCTTCCCGCGGAGCTCGAGCGAGTGGATGCGCTGT
TGGATGATCCAGCGTTCTTCGCACCGTTCGTTCCGTACTTCGACCCGCGGATCGGTCGTCCGTCGACGCCGATGGAGACCTACCTGCGGCTGATGTTCCT
CAAGTTCCGCTACCGGCTCGGCTACGAAAGCCTGTGCCGCGAGGTGAGCGACTCGATCACCTGGCGCCGGTTCTGCCGGATCCCCTTGGACGGGTCGGTG
CCGCACCCGACGACGTTGATGAAGCTGACCACCCGCTGTGGCGCGGCGGCGGTGGACGGGCTGAACGAGGCGCTGCTGGCCAAGGCCACCGAGGCCAAGG
TGCTGCGCACCACCACGTTGCGGGCCGACACCACCGTGGTGCCCAGCAACGTGTCCTACCCCACCGACTCCGGTTTGCTGGCCAAGGCAATCCGACGGAT
CGCGGCCACCGGCAAGCGGATCCAGGCCGCCGGTAGAGCGACACGCACCACGGTCCGGGACCGATCCCGTGCAGCGGGCAAGCGGGCGCACTCGATCGGT
TTCAAGCTGCGGTCGCGCAGCGCCGCCGGACGCGACGAGGCCCTGGCGGCGGTCCGGCGCACCACCGGGGAGCTGGCCGACCTGGCCGAGACCGCCGCCA
CCGATGCCGAACGGCTGCTGACCAACGCGAAACACGCGCTGCGCCGGGCACGCACCAAGGCCACAGCCCGGAAGGCCCAGGGCGAGCACGACGGGGCCGC
GGGCCGCCGGCGCGGACGCCTGGCCCGCGCCATCGACGACCTCGAGGACCTGGTCACCGCCACCCGGCAGATCACCGCGCAGACCCGACAGCGGCTGGCC
GGGCAGACCCCGGACGGAGCCACCCGGCGAGTCAGCCTGCACGACCCCGACGCCCGGCCCATCGCCAAGGGACGGCTCGGCAAACCGGTCGAGTTCGGCC
ACAAGACCCAGCTCGTCGAAGGCGACGACGGCGTGATCGTGGACCACAACGTCGAACGCGGCAACCCGGCCGACGCACCCCAATTGGCACCAGCCGTGGA
TCGGGTCCGCACACGCGCCGGCCGGCCACCCCGCACGGTCACCGCCGACCGCGGCTACGGCGAGAAAGCCGTCGAAGACGACCTACGCGACCTCGGCGTG
CGCCACGTCGTCATCCCCCGCAAGGGCAAACCCTCAGCTGACCGGCGAGCCGAGGAACACCGCCCAGCGTTCCGACGAACAATCAAGTGGCGCACCGGAG
TCGAAGGCAGAATCAGCGCCCTCAAACGTGGATATGGCTGGGACCGCACCCGCATCGACAGCACCGAAGGCGCCCAGATCTGGGTCGGCCACGGGGTCCT
GGCGCACAACCTGGTCAAGATCAGCACCCTGGCAGCGTGAAAGGACGCGGATCGGGGGGACTTGCGGCCAACCCAATCCCGACCGGCCGAGAACGGCCGA
ATCATGCTCACCGGGGCGCCTCGTTTTTCAGGTCGAAG
GTGTTCCGTACCGTAGGCGATCAACCGTCGTTGTTCGAGTCGGTGCTGCCGCAGGAGTTGCTGCGGCTTCCCGCGGAGCTCGAGCGAGTGGATGCGCTGT
TGGATGATCCAGCGTTCTTCGCACCGTTCGTTCCGTACTTCGACCCGCGGATCGGTCGTCCGTCGACGCCGATGGAGACCTACCTGCGGCTGATGTTCCT
CAAGTTCCGCTACCGGCTCGGCTACGAAAGCCTGTGCCGCGAGGTGAGCGACTCGATCACCTGGCGCCGGTTCTGCCGGATCCCCTTGGACGGGTCGGTG
CCGCACCCGACGACGTTGATGAAGCTGACCACCCGCTGTGGCGCGGCGGCGGTGGACGGGCTGAACGAGGCGCTGCTGGCCAAGGCCACCGAGGCCAAGG
TGCTGCGCACCACCACGTTGCGGGCCGACACCACCGTGGTGCCCAGCAACGTGTCCTACCCCACCGACTCCGGTTTGCTGGCCAAGGCAATCCGACGGAT
CGCGGCCACCGGCAAGCGGATCCAGGCCGCCGGTAGAGCGACACGCACCACGGTCCGGGACCGATCCCGTGCAGCGGGCAAGCGGGCGCACTCGATCGGT
TTCAAGCTGCGGTCGCGCAGCGCCGCCGGACGCGACGAGGCCCTGGCGGCGGTCCGGCGCACCACCGGGGAGCTGGCCGACCTGGCCGAGACCGCCGCCA
CCGATGCCGAACGGCTGCTGACCAACGCGAAACACGCGCTGCGCCGGGCACGCACCAAGGCCACAGCCCGGAAGGCCCAGGGCGAGCACGACGGGGCCGC
GGGCCGCCGGCGCGGACGCCTGGCCCGCGCCATCGACGACCTCGAGGACCTGGTCACCGCCACCCGGCAGATCACCGCGCAGACCCGACAGCGGCTGGCC
GGGCAGACCCCGGACGGAGCCACCCGGCGAGTCAGCCTGCACGACCCCGACGCCCGGCCCATCGCCAAGGGACGGCTCGGCAAACCGGTCGAGTTCGGCC
ACAAGACCCAGCTCGTCGAAGGCGACGACGGCGTGATCGTGGACCACAACGTCGAACGCGGCAACCCGGCCGACGCACCCCAATTGGCACCAGCCGTGGA
TCGGGTCCGCACACGCGCCGGCCGGCCACCCCGCACGGTCACCGCCGACCGCGGCTACGGCGAGAAAGCCGTCGAAGACGACCTACGCGACCTCGGCGTG
CGCCACGTCGTCATCCCCCGCAAGGGCAAACCCTCAGCTGACCGGCGAGCCGAGGAACACCGCCCAGCGTTCCGACGAACAATCAAGTGGCGCACCGGAG
TCGAAGGCAGAATCAGCGCCCTCAAACGTGGATATGGCTGGGACCGCACCCGCATCGACAGCACCGAAGGCGCCCAGATCTGGGTCGGCCACGGGGTCCT
GGCGCACAACCTGGTCAAGATCAGCACCCTGGCAGCGTGAAAGGACGCGGATCGGGGGGACTTGCGGCCAACCCAATCCCGACCGGCCGAGAACGGCCGA
ATCATGCTCACCGGGGCGCCTCGTTTTTCAGGTCGAAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1440 bp | 479 aa | 101 | 1540 | + | No |
Chemistry : DDE
ORF sequence :
VFRTVGDQPSLFESVLPQELLRLPAELERVDALLDDPAFFAPFVPYFDPRIGRPSTPMETYLRLMFLKFRYRLGYESLCREVSDSITWRRFCRIPLDGSV
PHPTTLMKLTTRCGAAAVDGLNEALLAKATEAKVLRTTTLRADTTVVPSNVSYPTDSGLLAKAIRRIAATGKRIQAAGRATRTTVRDRSRAAGKRAHSIG
FKLRSRSAAGRDEALAAVRRTTGELADLAETAATDAERLLTNAKHALRRARTKATARKAQGEHDGAAGRRRGRLARAIDDLEDLVTATRQITAQTRQRLA
GQTPDGATRRVSLHDPDARPIAKGRLGKPVEFGHKTQLVEGDDGVIVDHNVERGNPADAPQLAPAVDRVRTRAGRPPRTVTADRGYGEKAVEDDLRDLGV
RHVVIPRKGKPSADRRAEEHRPAFRRTIKWRTGVEGRISALKRGYGWDRTRIDSTEGAQIWVGHGVLAHNLVKISTLAA
PHPTTLMKLTTRCGAAAVDGLNEALLAKATEAKVLRTTTLRADTTVVPSNVSYPTDSGLLAKAIRRIAATGKRIQAAGRATRTTVRDRSRAAGKRAHSIG
FKLRSRSAAGRDEALAAVRRTTGELADLAETAATDAERLLTNAKHALRRARTKATARKAQGEHDGAAGRRRGRLARAIDDLEDLVTATRQITAQTRQRLA
GQTPDGATRRVSLHDPDARPIAKGRLGKPVEFGHKTQLVEGDDGVIVDHNVERGNPADAPQLAPAVDRVRTRAGRPPRTVTADRGYGEKAVEDDLRDLGV
RHVVIPRKGKPSADRRAEEHRPAFRRTIKWRTGVEGRISALKRGYGWDRTRIDSTEGAQIWVGHGVLAHNLVKISTLAA
Blast result :
Comments
ISRjo3 is 55% aa similar to ISCde2.
References
1] ISfinder annotation (2010).
2] McLeod,M.P., Warren,R.L., Hsiao,W.W., Araki,N., Myhre,M., Fernandes,C., Miyazawa,D., Wong,W., Lillquist,A.L., Wang,D., Dosanjh,M., Hara,H., Petrescu,A., Morin,R.D., Yang,G., Stott,J.M., Schein,J.E., Shin,H., Smailus,D., Siddiqui,A.S., Marra,M.A., Jones,S.J., Holt,R., Brinkman,F.S., Miyauchi,K., Fukuda,M., Davies,J.E., Mohn,W.W. and Eltis,L.D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15582-15587.
2] McLeod,M.P., Warren,R.L., Hsiao,W.W., Araki,N., Myhre,M., Fernandes,C., Miyazawa,D., Wong,W., Lillquist,A.L., Wang,D., Dosanjh,M., Hara,H., Petrescu,A., Morin,R.D., Yang,G., Stott,J.M., Schein,J.E., Shin,H., Smailus,D., Siddiqui,A.S., Marra,M.A., Jones,S.J., Holt,R., Brinkman,F.S., Miyauchi,K., Fukuda,M., Davies,J.E., Mohn,W.W. and Eltis,L.D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15582-15587.