ISLrh3
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_013198 | ND | Lactobacillus rhamnosus | Lactobacillus hilgardii ATCC 8290 Lactobacillus rhamnosus GG |
DNA section
IS Length : 1564 bp
Ends
IR Length : 15/19
IRL : GTGGCTGGTGAAAGCAGCATAACTGCAAGCTTTATTGGCATCAAAGAATA
IRR : GTGGCTGGTGAATTAGGCAACATTCCGGCTACCTGAAACGTGAATTTGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATAATCGAAC | TAATTA | GATACCAGTA | 6 |
| TCTTCACAAA | TAAATA | GCAACTTGAT | 6 |
| AAGCTATTTA | TAATTAGACT | 0 |
DNA sequence
GTGGCTGGTGAAAGCAGCATAACTGCAAGCTTTATTGGCATCAAAGAATAGTAAAGATTATAATTAGTGCAAAGTAAACATGGGCTAACGCCTGTTTTCC
TTGCACTTTTTATTTTTCCTGGAGGAAACCATCATGGCTTATCGACATCGTGCTACCCAATTGTCATTTCAATCCTTTAACAACGGCCTTGGTGTGCCAC
TCTCTTCCGACAATGAGTGGGTTCAATTAGCCGACATGCTCCCGTGGCAACAGCTCGATGAAGCCTATCAACTTCTTTTTACTGAGATGGGTGGGCGCGC
TGCTAAACCCTTTCGTCTGCTTTACGGTGCCAGTTTGATCAAGCAAGCAGAACATCTAACCGACCGTTCGGTCGTTACCGCTATTCGTGATACCCCGGCT
TACCAGTACTTTATCGGGCTAGACACCTACACAACCGACCTGCCGTTCAACCACTCAACCTTGGTTTATTTTAGACGACGCATGGGTCAAATAACGGAAC
TAGTGCGAAATATCATCAGTGATACCTTGCGCGAACAAATTCAAAGCTTATTGCCAGATGATGAGCTCCGTGTCTTGATCACAGACGCTACTGCGGTGCC
AATTGAAATTCGTTTTCCACAAGATACTTCGCTGCTAAATCAAGCTCGGCTCAATCTAGAAGAAATGTTATTAGACATGGCTCATCAGTTGCAAATCAAG
CCTCCACGAACCTACAAACGTGAAGCAAAAGCTAAGTGGACCGCTTTTGCTAGAAAACCGCGGCGTTGGGCTAAGGAAACACGCAAGCAGATCAAAGTAC
AGCTCCAGTATGTCCGACGTGATCTACGTTATATCGATGTGCTGTTGGCCCACGGTGCCTCTCTTAACGAAAGGCAAACCAAACGATTGGCTGTGATTCG
TGAACTTTTTGACCAGCAAATGTTCATGTATGAAAATCGGACCCATCGGGTCCCAGGACGGATTGTTAGCTTGGCACAACCGTGGATTCGCCCGATTAAC
CGTGGTAAAGCCAAACAACGAACCGAATTTGGTCCCAAAATTGATGCTTCAATTGCCGATGGCATGATCGATATCGAACGATTTGATTTTAAGGCGTTCA
ATGAAAGCCAAGATTTAGCAACAACCATCGATCATTATTTCGACGTGCATGGTTATTATCCCGATGAAATCTTAGCTGACACACTTTATCGTACCCGCGA
GAATCGTCAGCTCTGTCAACGACTAGGCATTAGGTTGTCGGGACCACGTTTAGGACGTAAGCCTAAAAAGATGGATGCTAAGCAGCGGCAAATTGATCGT
GACGCAGAGAAGCGGCGCGGGAAAATTGAACGCGGTTTTGCCTTCATGAAAGGCCCTTTGGGCCTTTCATTGGTGCGGACTAAAACGGTAGCCAGCATCG
CAGTGACGATTGATATTGCGATCAATCTAGCCAATCTAAAGATGCTATTAAGGCTTTTTAATGGACCAATTTTGATTTTTGTAAAATATAAGCAGGAATC
CATCTTAATTCACTATCAAATTCACGTTTCAGGTAGCCGGAATGTTGCCTAATTCACCAGCCAC
TTGCACTTTTTATTTTTCCTGGAGGAAACCATCATGGCTTATCGACATCGTGCTACCCAATTGTCATTTCAATCCTTTAACAACGGCCTTGGTGTGCCAC
TCTCTTCCGACAATGAGTGGGTTCAATTAGCCGACATGCTCCCGTGGCAACAGCTCGATGAAGCCTATCAACTTCTTTTTACTGAGATGGGTGGGCGCGC
TGCTAAACCCTTTCGTCTGCTTTACGGTGCCAGTTTGATCAAGCAAGCAGAACATCTAACCGACCGTTCGGTCGTTACCGCTATTCGTGATACCCCGGCT
TACCAGTACTTTATCGGGCTAGACACCTACACAACCGACCTGCCGTTCAACCACTCAACCTTGGTTTATTTTAGACGACGCATGGGTCAAATAACGGAAC
TAGTGCGAAATATCATCAGTGATACCTTGCGCGAACAAATTCAAAGCTTATTGCCAGATGATGAGCTCCGTGTCTTGATCACAGACGCTACTGCGGTGCC
AATTGAAATTCGTTTTCCACAAGATACTTCGCTGCTAAATCAAGCTCGGCTCAATCTAGAAGAAATGTTATTAGACATGGCTCATCAGTTGCAAATCAAG
CCTCCACGAACCTACAAACGTGAAGCAAAAGCTAAGTGGACCGCTTTTGCTAGAAAACCGCGGCGTTGGGCTAAGGAAACACGCAAGCAGATCAAAGTAC
AGCTCCAGTATGTCCGACGTGATCTACGTTATATCGATGTGCTGTTGGCCCACGGTGCCTCTCTTAACGAAAGGCAAACCAAACGATTGGCTGTGATTCG
TGAACTTTTTGACCAGCAAATGTTCATGTATGAAAATCGGACCCATCGGGTCCCAGGACGGATTGTTAGCTTGGCACAACCGTGGATTCGCCCGATTAAC
CGTGGTAAAGCCAAACAACGAACCGAATTTGGTCCCAAAATTGATGCTTCAATTGCCGATGGCATGATCGATATCGAACGATTTGATTTTAAGGCGTTCA
ATGAAAGCCAAGATTTAGCAACAACCATCGATCATTATTTCGACGTGCATGGTTATTATCCCGATGAAATCTTAGCTGACACACTTTATCGTACCCGCGA
GAATCGTCAGCTCTGTCAACGACTAGGCATTAGGTTGTCGGGACCACGTTTAGGACGTAAGCCTAAAAAGATGGATGCTAAGCAGCGGCAAATTGATCGT
GACGCAGAGAAGCGGCGCGGGAAAATTGAACGCGGTTTTGCCTTCATGAAAGGCCCTTTGGGCCTTTCATTGGTGCGGACTAAAACGGTAGCCAGCATCG
CAGTGACGATTGATATTGCGATCAATCTAGCCAATCTAAAGATGCTATTAAGGCTTTTTAATGGACCAATTTTGATTTTTGTAAAATATAAGCAGGAATC
CATCTTAATTCACTATCAAATTCACGTTTCAGGTAGCCGGAATGTTGCCTAATTCACCAGCCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1419 bp | 472 aa | 134 | 1552 | + | No |
Chemistry : DDE
ORF sequence :
MAYRHRATQLSFQSFNNGLGVPLSSDNEWVQLADMLPWQQLDEAYQLLFTEMGGRAAKPFRLLYGASLIKQAEHLTDRSVVTAIRDTPAYQYFIGLDTYT
TDLPFNHSTLVYFRRRMGQITELVRNIISDTLREQIQSLLPDDELRVLITDATAVPIEIRFPQDTSLLNQARLNLEEMLLDMAHQLQIKPPRTYKREAKA
KWTAFARKPRRWAKETRKQIKVQLQYVRRDLRYIDVLLAHGASLNERQTKRLAVIRELFDQQMFMYENRTHRVPGRIVSLAQPWIRPINRGKAKQRTEFG
PKIDASIADGMIDIERFDFKAFNESQDLATTIDHYFDVHGYYPDEILADTLYRTRENRQLCQRLGIRLSGPRLGRKPKKMDAKQRQIDRDAEKRRGKIER
GFAFMKGPLGLSLVRTKTVASIAVTIDIAINLANLKMLLRLFNGPILIFVKYKQESILIHYQIHVSGSRNVA
TDLPFNHSTLVYFRRRMGQITELVRNIISDTLREQIQSLLPDDELRVLITDATAVPIEIRFPQDTSLLNQARLNLEEMLLDMAHQLQIKPPRTYKREAKA
KWTAFARKPRRWAKETRKQIKVQLQYVRRDLRYIDVLLAHGASLNERQTKRLAVIRELFDQQMFMYENRTHRVPGRIVSLAQPWIRPINRGKAKQRTEFG
PKIDASIADGMIDIERFDFKAFNESQDLATTIDHYFDVHGYYPDEILADTLYRTRENRQLCQRLGIRLSGPRLGRKPKKMDAKQRQIDRDAEKRRGKIER
GFAFMKGPLGLSLVRTKTVASIAVTIDIAINLANLKMLLRLFNGPILIFVKYKQESILIHYQIHVSGSRNVA
Blast result :
Comments
ISLrh3 is 77% aa similar to ISLca2.
References
1] ISfinder annotation (2010)
2] Kankainen,M., Paulin,L., Tynkkynen,S., von Ossowski,I., Reunanen,J., Partanen,P., Satokari,R., Vesterlund,S., Hendrickx,A.P., Lebeer,S., De Keersmaecker,S.C., Vanderleyden,J., Hamalainen,T., Laukkanen,S., Salovuori,N., Ritari,J., Alatalo,E., Korpela,R., Mattila-Sandholm,T., Lassig,A., Hatakka,K., Kinnunen,K.T., Karjalainen,H., Saxelin,M., Laakso,K., Surakka,A., Palva,A., Salusjarvi,T., Auvinen,P. and de Vos,W.M. (2008) Direct submission GenBank.
3] Qin,X., Bachman,B., Battles,P., Bell,A., Bess,C., Bickham,C., Chaboub,L., Chen,D., Coyle,M., Deiros,D.R., Dinh,H., Forbes,L., Fowler,G., Francisco,L., Fu,Q., Gubbala,S., Hale,W., Han,Y., Hemphill,L., Highlander,S.K., Hirani,K., Hogues,M., Jackson,L., Jakkamsetti,A., Javaid,M., Jiang,H., Korchina,V., Kovar,C., Lara,F., Lee,S., Mata,R., Mathew,T., Moen,C., Morales,K., Munidasa,M., Nazareth,L., Ngo,R., Nguyen,L., Okwuonu,G., Ongeri,F., Patil,S., Petrosino,J., Pham,C., Pham,P., Pu,L.-L., Puazo,M., Raj,R., Reid,J., Rouhana,J., Saada,N., Shang,Y., Simmons,D., Thornton,R., Warren,J., Weissenberger,G., Zhang,J., Zhang,L., Zhou,C., Zhu,D., Muzny,D., Worley,K. and Gibbs,R. (2009) Direct submission Genbank.
2] Kankainen,M., Paulin,L., Tynkkynen,S., von Ossowski,I., Reunanen,J., Partanen,P., Satokari,R., Vesterlund,S., Hendrickx,A.P., Lebeer,S., De Keersmaecker,S.C., Vanderleyden,J., Hamalainen,T., Laukkanen,S., Salovuori,N., Ritari,J., Alatalo,E., Korpela,R., Mattila-Sandholm,T., Lassig,A., Hatakka,K., Kinnunen,K.T., Karjalainen,H., Saxelin,M., Laakso,K., Surakka,A., Palva,A., Salusjarvi,T., Auvinen,P. and de Vos,W.M. (2008) Direct submission GenBank.
3] Qin,X., Bachman,B., Battles,P., Bell,A., Bess,C., Bickham,C., Chaboub,L., Chen,D., Coyle,M., Deiros,D.R., Dinh,H., Forbes,L., Fowler,G., Francisco,L., Fu,Q., Gubbala,S., Hale,W., Han,Y., Hemphill,L., Highlander,S.K., Hirani,K., Hogues,M., Jackson,L., Jakkamsetti,A., Javaid,M., Jiang,H., Korchina,V., Kovar,C., Lara,F., Lee,S., Mata,R., Mathew,T., Moen,C., Morales,K., Munidasa,M., Nazareth,L., Ngo,R., Nguyen,L., Okwuonu,G., Ongeri,F., Patil,S., Petrosino,J., Pham,C., Pham,P., Pu,L.-L., Puazo,M., Raj,R., Reid,J., Rouhana,J., Saada,N., Shang,Y., Simmons,D., Thornton,R., Warren,J., Weissenberger,G., Zhang,J., Zhang,L., Zhou,C., Zhu,D., Muzny,D., Worley,K. and Gibbs,R. (2009) Direct submission Genbank.