ISBco3
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAWV02000001 | ND | Bacillus coagulans | Bacillus coagulans 36D1 |
DNA section
IS Length : 1668 bp
Ends
IR Length : 14/16
IRL : GGGCTTGCTGAATAAACAAAAATTCCAGATTTGACAAGGATTTTGGCCAC
IRR : GGGCTTGCTGAACGAATCAGATTCTGTCAGATACAAGCCAAATTACGGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGTTAAAAAC | CACTTA | TAACTTTCAG | 6 |
DNA sequence
GGGCTTGCTGAATAAACAAAAATTCCAGATTTGACAAGGATTTTGGCCACTTTTGTCGAATATAAAGTGTAAGGATTTTATAGGAAACGTATAAAAAACC
TTGCACTTGGAGGCCGTTACGATGTACAAAAAGACCGAACATCAATTAACTTTTGTAGAAGATTTTTTTCTACCATTCGGTGGAAAATTAAATAAAGAAA
ATCGCTGGGTCCGTCTTGCTGAATTAATCCCTTGGTGGAAGGCTGAAGAAAAGTATGCCAAGTCCTTCAAGAAAAAATTCAAAGGTGAAAAGGCTTATTC
TGTCAGAGTTGCTCTTGGGGCTCTCTATATCAAAGAACGGCTTGGGCTATCTGACCGAGAAACAGTCGAACAGATCACGGAAAACCCGTATCTTCAGTAT
TTTATCGGGTTGCCTGAATTCCAGGAAAAAGCCCCATTCGATCATTCTCTGATGACTCGTTTTAGAAAACGTTTGGGTGCAAACATCATCAATGAACTGA
ATGAATGGATTGTTCTGGAAGAACAAGAACGCCAATCGGAAGAAGCATCTAAGGAAAATGACAACGATGATGATCACCATGATGATGACGATCATAGTAG
TGGTGATCATCAACCTTCCGGTCCTGCTTCGGAAGAGAATTCAACTGGATCGGCTTCTCCTAAAACGAAGAACAAAGGAAAGCTCATTCTGGACGCAACC
TGTGCACCGGCTGATATTGCTTACCCTACTGACCTGGGGCTTTTGAACGAAGCCCGTGAGAAACTGGAACACATCATTGATGTACTGCATGAACCGCACA
AAGGAAAATTACGAAAGCCACGGACCTACAGAAAAAGAGCCCGCAGGGACTATCTTTCTGTCGCTAAGCAACGCAAAGCTGGAGCCCGGAAAATCCGGAA
AGCTGTCGGCAAACAACTTGGATATGTGAAACGGAATCTGGAGATCATTGGAAATCTCATTTCCCAAAGCTCCCTGTCTCTTTTGAGCAAAGATGAATAT
CGACAACTCCTCGTGATTCATGAACTTTACCGCCAACAGGCAGAAATGTACAAAAAGAAAACACATCGGATTGACCACCGGATTGTGAGCATTTCACAGC
CGCATGTCCGTCCAATTGTGCGTGGCAAGGCAAAAGCGAACGTGGAATTCGGCTCAAAAGTCGCCATTAGCGTTGTAGATGGCTATGCCTTGATTGAGCA
ACTCGACTGGGAAAATTACAATGAAGGGATCACCCTTAGAGAATCTATTGAGAACTACTACAACCGGTTCGGATTTTATCCAGAAGCGATTTTAGCAGAT
AAAATCTACCGAAACCGTCACAACCTAGCATACTGTAAATCTCTTGGCATTCGTCTGAGTGGGCCAAGGTTGGGACGCCCATCCAAAACGGAAGACAAAA
CCTTAGAACGCGTGGCAAGGCAGGATGCCTCCGAACGTAATGCCGTTGAAAGCAAATTTGGCGAAGGCAAGCGCAAGTATGGGCTAGGCCTTATTCGAGC
ACGCCTTCAGGAGACCAGTGAAACAGTAGTAGCCCTTCAATTCCTTATAATGAATCTGGAGCGGAAGCTCAGGGTTCTTTTTTTGAAATTTTTGCACAAC
ACTATTTTATATTTTAATAACCGTAATTTGGCTTGTATCTGACAGAATCTGATTCGTTCAGCAAGCCC
TTGCACTTGGAGGCCGTTACGATGTACAAAAAGACCGAACATCAATTAACTTTTGTAGAAGATTTTTTTCTACCATTCGGTGGAAAATTAAATAAAGAAA
ATCGCTGGGTCCGTCTTGCTGAATTAATCCCTTGGTGGAAGGCTGAAGAAAAGTATGCCAAGTCCTTCAAGAAAAAATTCAAAGGTGAAAAGGCTTATTC
TGTCAGAGTTGCTCTTGGGGCTCTCTATATCAAAGAACGGCTTGGGCTATCTGACCGAGAAACAGTCGAACAGATCACGGAAAACCCGTATCTTCAGTAT
TTTATCGGGTTGCCTGAATTCCAGGAAAAAGCCCCATTCGATCATTCTCTGATGACTCGTTTTAGAAAACGTTTGGGTGCAAACATCATCAATGAACTGA
ATGAATGGATTGTTCTGGAAGAACAAGAACGCCAATCGGAAGAAGCATCTAAGGAAAATGACAACGATGATGATCACCATGATGATGACGATCATAGTAG
TGGTGATCATCAACCTTCCGGTCCTGCTTCGGAAGAGAATTCAACTGGATCGGCTTCTCCTAAAACGAAGAACAAAGGAAAGCTCATTCTGGACGCAACC
TGTGCACCGGCTGATATTGCTTACCCTACTGACCTGGGGCTTTTGAACGAAGCCCGTGAGAAACTGGAACACATCATTGATGTACTGCATGAACCGCACA
AAGGAAAATTACGAAAGCCACGGACCTACAGAAAAAGAGCCCGCAGGGACTATCTTTCTGTCGCTAAGCAACGCAAAGCTGGAGCCCGGAAAATCCGGAA
AGCTGTCGGCAAACAACTTGGATATGTGAAACGGAATCTGGAGATCATTGGAAATCTCATTTCCCAAAGCTCCCTGTCTCTTTTGAGCAAAGATGAATAT
CGACAACTCCTCGTGATTCATGAACTTTACCGCCAACAGGCAGAAATGTACAAAAAGAAAACACATCGGATTGACCACCGGATTGTGAGCATTTCACAGC
CGCATGTCCGTCCAATTGTGCGTGGCAAGGCAAAAGCGAACGTGGAATTCGGCTCAAAAGTCGCCATTAGCGTTGTAGATGGCTATGCCTTGATTGAGCA
ACTCGACTGGGAAAATTACAATGAAGGGATCACCCTTAGAGAATCTATTGAGAACTACTACAACCGGTTCGGATTTTATCCAGAAGCGATTTTAGCAGAT
AAAATCTACCGAAACCGTCACAACCTAGCATACTGTAAATCTCTTGGCATTCGTCTGAGTGGGCCAAGGTTGGGACGCCCATCCAAAACGGAAGACAAAA
CCTTAGAACGCGTGGCAAGGCAGGATGCCTCCGAACGTAATGCCGTTGAAAGCAAATTTGGCGAAGGCAAGCGCAAGTATGGGCTAGGCCTTATTCGAGC
ACGCCTTCAGGAGACCAGTGAAACAGTAGTAGCCCTTCAATTCCTTATAATGAATCTGGAGCGGAAGCTCAGGGTTCTTTTTTTGAAATTTTTGCACAAC
ACTATTTTATATTTTAATAACCGTAATTTGGCTTGTATCTGACAGAATCTGATTCGTTCAGCAAGCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1521 bp | 506 aa | 122 | 1642 | + | No |
Chemistry : DDE
ORF sequence :
MYKKTEHQLTFVEDFFLPFGGKLNKENRWVRLAELIPWWKAEEKYAKSFKKKFKGEKAYSVRVALGALYIKERLGLSDRETVEQITENPYLQYFIGLPEF
QEKAPFDHSLMTRFRKRLGANIINELNEWIVLEEQERQSEEASKENDNDDDHHDDDDHSSGDHQPSGPASEENSTGSASPKTKNKGKLILDATCAPADIA
YPTDLGLLNEAREKLEHIIDVLHEPHKGKLRKPRTYRKRARRDYLSVAKQRKAGARKIRKAVGKQLGYVKRNLEIIGNLISQSSLSLLSKDEYRQLLVIH
ELYRQQAEMYKKKTHRIDHRIVSISQPHVRPIVRGKAKANVEFGSKVAISVVDGYALIEQLDWENYNEGITLRESIENYYNRFGFYPEAILADKIYRNRH
NLAYCKSLGIRLSGPRLGRPSKTEDKTLERVARQDASERNAVESKFGEGKRKYGLGLIRARLQETSETVVALQFLIMNLERKLRVLFLKFLHNTILYFNN
RNLACI
QEKAPFDHSLMTRFRKRLGANIINELNEWIVLEEQERQSEEASKENDNDDDHHDDDDHSSGDHQPSGPASEENSTGSASPKTKNKGKLILDATCAPADIA
YPTDLGLLNEAREKLEHIIDVLHEPHKGKLRKPRTYRKRARRDYLSVAKQRKAGARKIRKAVGKQLGYVKRNLEIIGNLISQSSLSLLSKDEYRQLLVIH
ELYRQQAEMYKKKTHRIDHRIVSISQPHVRPIVRGKAKANVEFGSKVAISVVDGYALIEQLDWENYNEGITLRESIENYYNRFGFYPEAILADKIYRNRH
NLAYCKSLGIRLSGPRLGRPSKTEDKTLERVARQDASERNAVESKFGEGKRKYGLGLIRARLQETSETVVALQFLIMNLERKLRVLFLKFLHNTILYFNN
RNLACI
Blast result :
Comments
ISBco3 is 69% aa similar to ISPeth2.
References
1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Chertkov,O., Brettin,T., Han,C., Detter,C., Pitluck,S., Patel,M., Ou,M., Rhee,M.S., Harbrucker,R., Ingram,L.O. and Shanmugam,K.T. (2009) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Chertkov,O., Brettin,T., Han,C., Detter,C., Pitluck,S., Patel,M., Ou,M., Rhee,M.S., Harbrucker,R., Ingram,L.O. and Shanmugam,K.T. (2009) Direct submission GenBank.