ISLru1
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DS180869 | ND | Leptospirillum rubarum | Leptospirillum rubarum |
DNA section
IS Length : 1608 bp
Ends
IR Length : 14/17
IRL : GTTCATACTGAAAAACTAGTCACGTAAGTAATTGACAAATATATATAGTT
IRR : GTTGACGCTGAAAAACTCACTTTTTTTAAAGAGGCCCAAGAATCATGAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGTGTCCTT | TAACTA | AAAGAAAGGT | 6 |
| TTATTTCCTT | TAATTA | AAGAAGACAT | 6 |
DNA sequence
GTTCATACTGAAAAACTAGTCACGTAAGTAATTGACAAATATATATAGTTAAGTTATGATCCCTCTTGGAAATGGAGGAATTTTCACCTTTTTTCCGAGA
GGACGTTCCATGGCGAAATCGCCCCATACCCAACCGAGCCTGTTCCAGTGGATCGCCGACCGGCATGTAAATCCAGAGCATCCTCTGATGAAGCTCTCGG
AGAAGATCGACTGGGCGGCGATCGACGGGGTTTTGGCTCCGTACTATTCTCCCGGGAAGGGACGGCCGGCCAAGCCGACACGGCTTATGGTGGGTCTCAT
GATCCTGAAACACCGGTTCGACCTCTCGGACGAAGCGGTGGTCTCGGGGCTTCACGAGAACTTCGTGTGGATGGCCTTTTGCCGGGTTCCGGCCGACGAA
GCCCAGCACGTCGAGTCCTCCACGCTGTGCAAGTTTCGGAAGCGTCTCGGACCAGAGGGAACGCAGGCAGTGGAGGCGGTCATCCGGGACCAGATGATCC
AGGCCGGAGCGATCCGGCGCCAGGTCATGCGCGTCGACACCACGGCGATGGAGAAGAACATCGCCTACCCGACGGACGTAGGACTGCTTCAAAAGTCCCG
GACGCGGGTGCTTCGGCTGGTCCAAAAAGCCAAGGCGCTGGGAGTGGCTGTCCCCCGAACCCTCCGGACCTTTGTCCGGGTGAGCAAGAAGGTGGTGCTG
TCGGCCGCGAAGTTTGGACGGGACAAGAAGGAACGGTCCCAAAAGGCCGCCAAGAAGCTTTCCTCCATGGGGGAGCACACGCTCCGGCGCGTCGCAGGAA
TCGGGCGGGAAATCGGAAAGAGTCTCCGGAAACCGGCTGTCCGGACCAAGCCATCTGTCCGACGGAGCCTGATCCAGATCCGGGAAGGCCTCCAGAAGGA
GGTCCGGATCCTGGAGCATATCGTGGTCCAGACGAAGGACCGGCTCAAGAATCGTCCGACGAAAAAGCGGATCTACAGCGTCCATGAGCCGGAGGTCACC
TGCATCACGAAAAACAAGGCGGGCAAGCCCCACGAGTACGGGGTCAAGGTGGCCCTGGCGGTTTCGGAGAATGGGATGGTGGTGACCCATGCCGAGTACG
CCGACAACCGGCATGACTCCACAACGCTGGCCGATTCCCTGACGGGATGGGAAGAGGCGACGGGAGGTCTTCCGACGGCGCTTGCGGCGGACCGGGGATA
CGGCACCCAAGACAAGAACACGCCTCCGGCTCTTTCCCGGATCCCGAAGGTGGCCATTCCCAAAAAGGGAAAAATTCGGGGACCGCTGGAACACACCCGG
TGGTTCAAGAGGCTGGTCGCCTCCCGGGCGGGAATCGAGGCGGTGATCGCCCACCTGAAGCAAGATCATGGGATGGGACGATGCCGGTACAAGGGGTTTT
CCGGGGACCAGATGAACGTCTCCTGGGCGGTTCTGGCCTGGAATACCAAAAAGTGGGTGTCCCAGACGACCGGATAAGGGCGAAGGACCTCCCGACCAGG
ACAAAATCGGGGTGTTCCGGAAAGAACGCTTGATTTTATCTGTCCTTCCCCGATCCGGATTCATGATTCTTGGGCCTCTTTAAAAAAAGTGAGTTTTTCA
GCGTCAAC
GGACGTTCCATGGCGAAATCGCCCCATACCCAACCGAGCCTGTTCCAGTGGATCGCCGACCGGCATGTAAATCCAGAGCATCCTCTGATGAAGCTCTCGG
AGAAGATCGACTGGGCGGCGATCGACGGGGTTTTGGCTCCGTACTATTCTCCCGGGAAGGGACGGCCGGCCAAGCCGACACGGCTTATGGTGGGTCTCAT
GATCCTGAAACACCGGTTCGACCTCTCGGACGAAGCGGTGGTCTCGGGGCTTCACGAGAACTTCGTGTGGATGGCCTTTTGCCGGGTTCCGGCCGACGAA
GCCCAGCACGTCGAGTCCTCCACGCTGTGCAAGTTTCGGAAGCGTCTCGGACCAGAGGGAACGCAGGCAGTGGAGGCGGTCATCCGGGACCAGATGATCC
AGGCCGGAGCGATCCGGCGCCAGGTCATGCGCGTCGACACCACGGCGATGGAGAAGAACATCGCCTACCCGACGGACGTAGGACTGCTTCAAAAGTCCCG
GACGCGGGTGCTTCGGCTGGTCCAAAAAGCCAAGGCGCTGGGAGTGGCTGTCCCCCGAACCCTCCGGACCTTTGTCCGGGTGAGCAAGAAGGTGGTGCTG
TCGGCCGCGAAGTTTGGACGGGACAAGAAGGAACGGTCCCAAAAGGCCGCCAAGAAGCTTTCCTCCATGGGGGAGCACACGCTCCGGCGCGTCGCAGGAA
TCGGGCGGGAAATCGGAAAGAGTCTCCGGAAACCGGCTGTCCGGACCAAGCCATCTGTCCGACGGAGCCTGATCCAGATCCGGGAAGGCCTCCAGAAGGA
GGTCCGGATCCTGGAGCATATCGTGGTCCAGACGAAGGACCGGCTCAAGAATCGTCCGACGAAAAAGCGGATCTACAGCGTCCATGAGCCGGAGGTCACC
TGCATCACGAAAAACAAGGCGGGCAAGCCCCACGAGTACGGGGTCAAGGTGGCCCTGGCGGTTTCGGAGAATGGGATGGTGGTGACCCATGCCGAGTACG
CCGACAACCGGCATGACTCCACAACGCTGGCCGATTCCCTGACGGGATGGGAAGAGGCGACGGGAGGTCTTCCGACGGCGCTTGCGGCGGACCGGGGATA
CGGCACCCAAGACAAGAACACGCCTCCGGCTCTTTCCCGGATCCCGAAGGTGGCCATTCCCAAAAAGGGAAAAATTCGGGGACCGCTGGAACACACCCGG
TGGTTCAAGAGGCTGGTCGCCTCCCGGGCGGGAATCGAGGCGGTGATCGCCCACCTGAAGCAAGATCATGGGATGGGACGATGCCGGTACAAGGGGTTTT
CCGGGGACCAGATGAACGTCTCCTGGGCGGTTCTGGCCTGGAATACCAAAAAGTGGGTGTCCCAGACGACCGGATAAGGGCGAAGGACCTCCCGACCAGG
ACAAAATCGGGGTGTTCCGGAAAGAACGCTTGATTTTATCTGTCCTTCCCCGATCCGGATTCATGATTCTTGGGCCTCTTTAAAAAAAGTGAGTTTTTCA
GCGTCAAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1368 bp | 455 aa | 110 | 1477 | + | No |
Chemistry : DDE
ORF sequence :
MAKSPHTQPSLFQWIADRHVNPEHPLMKLSEKIDWAAIDGVLAPYYSPGKGRPAKPTRLMVGLMILKHRFDLSDEAVVSGLHENFVWMAFCRVPADEAQH
VESSTLCKFRKRLGPEGTQAVEAVIRDQMIQAGAIRRQVMRVDTTAMEKNIAYPTDVGLLQKSRTRVLRLVQKAKALGVAVPRTLRTFVRVSKKVVLSAA
KFGRDKKERSQKAAKKLSSMGEHTLRRVAGIGREIGKSLRKPAVRTKPSVRRSLIQIREGLQKEVRILEHIVVQTKDRLKNRPTKKRIYSVHEPEVTCIT
KNKAGKPHEYGVKVALAVSENGMVVTHAEYADNRHDSTTLADSLTGWEEATGGLPTALAADRGYGTQDKNTPPALSRIPKVAIPKKGKIRGPLEHTRWFK
RLVASRAGIEAVIAHLKQDHGMGRCRYKGFSGDQMNVSWAVLAWNTKKWVSQTTG
VESSTLCKFRKRLGPEGTQAVEAVIRDQMIQAGAIRRQVMRVDTTAMEKNIAYPTDVGLLQKSRTRVLRLVQKAKALGVAVPRTLRTFVRVSKKVVLSAA
KFGRDKKERSQKAAKKLSSMGEHTLRRVAGIGREIGKSLRKPAVRTKPSVRRSLIQIREGLQKEVRILEHIVVQTKDRLKNRPTKKRIYSVHEPEVTCIT
KNKAGKPHEYGVKVALAVSENGMVVTHAEYADNRHDSTTLADSLTGWEEATGGLPTALAADRGYGTQDKNTPPALSRIPKVAIPKKGKIRGPLEHTRWFK
RLVASRAGIEAVIAHLKQDHGMGRCRYKGFSGDQMNVSWAVLAWNTKKWVSQTTG
Blast result :
Comments
ISLru1 is 48% aa siilar to ISBpl1.
References
1] ISfinder annotation (2010)
2] Lo,I., Denef,V.J., Verberkmoes,N.C., Shah,M.B., Goltsman,D., DiBartolo,G., Tyson,G.W., Allen,E.E., Ram,R.J., Detter,J.C., Richardson,P., Thelen,M.P., Hettich,R.L. and Banfield,J.F. (2007) Nature 446 (7135), 537-541.
3] Goltsman,D.S., Denef,V.J., Singer,S.W., VerBerkmoes,N.C., Lefsrud,M., Mueller,R.S., Dick,G.J., Sun,C.L., Wheeler,K.E., Zemla,A., Baker,B.J., Hauser,L., Land,M., Shah,M.B., Thelen,M.P., Hettich,R.L. and Banfield,J.F. (2009)Appl. Environ. Microbiol. 75 (13), 4599-4615.
2] Lo,I., Denef,V.J., Verberkmoes,N.C., Shah,M.B., Goltsman,D., DiBartolo,G., Tyson,G.W., Allen,E.E., Ram,R.J., Detter,J.C., Richardson,P., Thelen,M.P., Hettich,R.L. and Banfield,J.F. (2007) Nature 446 (7135), 537-541.
3] Goltsman,D.S., Denef,V.J., Singer,S.W., VerBerkmoes,N.C., Lefsrud,M., Mueller,R.S., Dick,G.J., Sun,C.L., Wheeler,K.E., Zemla,A., Baker,B.J., Hauser,L., Land,M., Shah,M.B., Thelen,M.P., Hettich,R.L. and Banfield,J.F. (2009)Appl. Environ. Microbiol. 75 (13), 4599-4615.