ISBj2_B
- Family IS5
- Group IS5
Isoform Synonym(s) ISBj15
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004463 | ND | Bradyrhizobium japonicum | Bradyrhizobium japonicum USDA 110 |
DNA section
IS Length : 1509 bp
Ends
IR Length : 13/18
IRL : GTTGTCCGTGAACAAGCCTCCAAGCACTTGATTGAGAGTCTGTTTTCCGG
IRR : GTCGATCGTTAATAAGCCGAATTGGGCTGAACTGGACAGTTGAGCGTTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGATCAGCT | TCGGAT | CGCAGGCAGC | 6 |
DNA sequence
GTTGTCCGTGAACAAGCCTCCAAGCACTTGATTGAGAGTCTGTTTTCCGGTTGCGGCGGCATTTGAGATTGCAGGGGATTGGGGCTTCACAGCAAAAACC
TGCAATTTCGATTTTGGATTTCCCTGCCGCTGCGAACCGTGATTCTGTGCTGCATCGGAGGGACCGATGCGACCAAAGAAGCACAGGACGACAGGCTCGA
ACGATCTGTTCCGGTCCCGGCTGGACCAGATCATCAACATGCGACACGAGCTGGTTCTGCTCGCCGGCAGGGTCGATTGGGACTGGATCGACGGCGAGAT
CGCGCCGCTCTACAGCGACAACGGCAGGCCGGGTATCGCGACCCGCTTCATGATCGGATTGCTGCTGCTCAAGCACATCTACGAGGGGGTGTGCGAGCGC
TGGGTCCACGATCCGTACTTCCAGTTCTTCACCGGCGAAGAGTTTTTCCAGCACGTTTTCCCGCATGAGCGCTCAGACCTGAGCCACTGGCGCAAGCGGC
TCGGCGACAAGCTGGAGCGGTTGTTGGCCGAGAGCCTTCGCGTGGCGCACGCGAGCGGTGCGTTGCGCAGCCAGGACCTCAAGCGGGTCACGGTCGACAC
GACCGTCCAACCGAAGGCCATCAGCTTCCCGACCGACGCCAAGCTGCTGCATGCAGCGATCCGCGGGTTGAACCGCCTGGCGAGGAAGCACGGGGTCAGG
CTCCGGCAATCCTATGTTCGCGTGGCCAAAAGCGCGGCGATGATGGCGGGCCGCTACGCTCATGCCAAACAATTCAACCGGCATCAGCGACAGCTGCGCA
TCCTGCGCAGCCGGCTGGGCCGGATCATCCGCGACATTCGCCGCAAGACCGAGGGCCAGGCCGCATTGGAGGGGGCATTCGCCCTGCCGCTGAGCCGAGC
CACCCAGATCGGCTCGCAGCAGCAGCGCCAGCGCGGCTGGAAGTTGTATTCCTTCCATGCTCCGGAGGTCGAGTGCATCGGCAAGGGCAAGGCCGCCGCG
CCCTATGAGTTCTGCGTGAAGGCCTCCATCGTCACCAACAACCAAAGAGCTCCGGGCGGCTTGTTCGTGCTCCACGCCTGCTCGCTGCCGGACAACCCCT
ACGACGGTCACACCCTTCGCAACGTCATCGAGCGCACCGAGAGCCTCACCGGCTGCCCGATCGAACGGGCCTATGTCGACAAGGGATATCGCGGCCACGA
CACACAAAATCCCCGCCGCGTCTTCATCTCGGGCCAGAAGCGCGGCGTCTTCGGCGTCATCAAGCGCGAGCTAGGCCGCCGTTCCGCCATCGAGCCCATC
ATCGGACACATGAAGAACGAAGGTCACCTTGGCCGCTGCTATCTCAAAGGCCGCGCCGGAGATGCCGCTAACGTTCTCCTCTCGGCCGTTGGCCACAACC
TCCGCCGTGTCCTGGCTTGGCTCAGAGACCTTTTGTCCCTCTTCCTGCTCTCACTCTGGCCAACGCTCAACTGTCCAGTTCAGCCCAATTCGGCTTATTA
ACGATCGAC
TGCAATTTCGATTTTGGATTTCCCTGCCGCTGCGAACCGTGATTCTGTGCTGCATCGGAGGGACCGATGCGACCAAAGAAGCACAGGACGACAGGCTCGA
ACGATCTGTTCCGGTCCCGGCTGGACCAGATCATCAACATGCGACACGAGCTGGTTCTGCTCGCCGGCAGGGTCGATTGGGACTGGATCGACGGCGAGAT
CGCGCCGCTCTACAGCGACAACGGCAGGCCGGGTATCGCGACCCGCTTCATGATCGGATTGCTGCTGCTCAAGCACATCTACGAGGGGGTGTGCGAGCGC
TGGGTCCACGATCCGTACTTCCAGTTCTTCACCGGCGAAGAGTTTTTCCAGCACGTTTTCCCGCATGAGCGCTCAGACCTGAGCCACTGGCGCAAGCGGC
TCGGCGACAAGCTGGAGCGGTTGTTGGCCGAGAGCCTTCGCGTGGCGCACGCGAGCGGTGCGTTGCGCAGCCAGGACCTCAAGCGGGTCACGGTCGACAC
GACCGTCCAACCGAAGGCCATCAGCTTCCCGACCGACGCCAAGCTGCTGCATGCAGCGATCCGCGGGTTGAACCGCCTGGCGAGGAAGCACGGGGTCAGG
CTCCGGCAATCCTATGTTCGCGTGGCCAAAAGCGCGGCGATGATGGCGGGCCGCTACGCTCATGCCAAACAATTCAACCGGCATCAGCGACAGCTGCGCA
TCCTGCGCAGCCGGCTGGGCCGGATCATCCGCGACATTCGCCGCAAGACCGAGGGCCAGGCCGCATTGGAGGGGGCATTCGCCCTGCCGCTGAGCCGAGC
CACCCAGATCGGCTCGCAGCAGCAGCGCCAGCGCGGCTGGAAGTTGTATTCCTTCCATGCTCCGGAGGTCGAGTGCATCGGCAAGGGCAAGGCCGCCGCG
CCCTATGAGTTCTGCGTGAAGGCCTCCATCGTCACCAACAACCAAAGAGCTCCGGGCGGCTTGTTCGTGCTCCACGCCTGCTCGCTGCCGGACAACCCCT
ACGACGGTCACACCCTTCGCAACGTCATCGAGCGCACCGAGAGCCTCACCGGCTGCCCGATCGAACGGGCCTATGTCGACAAGGGATATCGCGGCCACGA
CACACAAAATCCCCGCCGCGTCTTCATCTCGGGCCAGAAGCGCGGCGTCTTCGGCGTCATCAAGCGCGAGCTAGGCCGCCGTTCCGCCATCGAGCCCATC
ATCGGACACATGAAGAACGAAGGTCACCTTGGCCGCTGCTATCTCAAAGGCCGCGCCGGAGATGCCGCTAACGTTCTCCTCTCGGCCGTTGGCCACAACC
TCCGCCGTGTCCTGGCTTGGCTCAGAGACCTTTTGTCCCTCTTCCTGCTCTCACTCTGGCCAACGCTCAACTGTCCAGTTCAGCCCAATTCGGCTTATTA
ACGATCGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 167 | 1501 | + | No |
Chemistry : DDE
ORF sequence :
MRPKKHRTTGSNDLFRSRLDQIINMRHELVLLAGRVDWDWIDGEIAPLYSDNGRPGIATRFMIGLLLLKHIYEGVCERWVHDPYFQFFTGEEFFQHVFPH
ERSDLSHWRKRLGDKLERLLAESLRVAHASGALRSQDLKRVTVDTTVQPKAISFPTDAKLLHAAIRGLNRLARKHGVRLRQSYVRVAKSAAMMAGRYAHA
KQFNRHQRQLRILRSRLGRIIRDIRRKTEGQAALEGAFALPLSRATQIGSQQQRQRGWKLYSFHAPEVECIGKGKAAAPYEFCVKASIVTNNQRAPGGLF
VLHACSLPDNPYDGHTLRNVIERTESLTGCPIERAYVDKGYRGHDTQNPRRVFISGQKRGVFGVIKRELGRRSAIEPIIGHMKNEGHLGRCYLKGRAGDA
ANVLLSAVGHNLRRVLAWLRDLLSLFLLSLWPTLNCPVQPNSAY
ERSDLSHWRKRLGDKLERLLAESLRVAHASGALRSQDLKRVTVDTTVQPKAISFPTDAKLLHAAIRGLNRLARKHGVRLRQSYVRVAKSAAMMAGRYAHA
KQFNRHQRQLRILRSRLGRIIRDIRRKTEGQAALEGAFALPLSRATQIGSQQQRQRGWKLYSFHAPEVECIGKGKAAAPYEFCVKASIVTNNQRAPGGLF
VLHACSLPDNPYDGHTLRNVIERTESLTGCPIERAYVDKGYRGHDTQNPRRVFISGQKRGVFGVIKRELGRRSAIEPIIGHMKNEGHLGRCYLKGRAGDA
ANVLLSAVGHNLRRVLAWLRDLLSLFLLSLWPTLNCPVQPNSAY
Blast result :
Comments
ISBj2_B is 92% aa similar to ISBj5.
References
1] ISfinder annotation (2010)
2] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 225-256.
3] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 189-197.
2] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 225-256.
3] Kaneko,T., Nakamura,Y., Sato,S., Minamisawa,K., Uchiumi,T., Sasamoto,S., Watanabe,A., Idesawa,K., Iriguchi,M., Kawashima,K., Kohara,M., Matsumoto,M., Shimpo,S., Tsuruoka,H., Wada,T., Yamada,M. and Tabata,S. (2002) DNA Res. 9 (6), 189-197.