ISfinder Mirror Site

ISRpa8

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007925	ND	Rhodopseudomonas palustris	Rhodopseudomonas palustris BisB18

DNA section

IS Length : 1540 bp

Ends

IR Length : 21/26

IRL : GTCGTCCGTGAACAATTCGGAAGCGCCTGATTTTTCGGGCTAATCGAGTT
IRR : GTCGTCCGTGAAGATCCCGGACACGCGCCGATTTAGGCGTATTGCATCGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGGCTCTAAA		CAAAAACGCC	0

DNA sequence

GTCGTCCGTGAACAATTCGGAAGCGCCTGATTTTTCGGGCTAATCGAGTTCGTGCAAGTCTTCGATTTTGCAGGAAAGCGGTGGGTTGACTGCGGTTTTC
CTGCGTTTTGGGATTCTCATTTTGGGCGGTTTGTGGTTCCTTTGGGCGGCGTTTTGCTGGCATGGAGGGGGCGATGAGCAAGCCGCGCGACGATCGTCAG
GCCGAGATGTTCCGGCCGGCGCTGGAAGAGATCATCGATCTCGGGCATCCGTTGGTGCGGCTGGCGGCAGAGATCGACTGGGATTTCCTGGCCACGCGGT
TTGGCGCGGTGTATCGGCCGGGGCCGGGCCAGCCGCCATTGCCGACCCGGCTGATCGCCGGCCTGTTGATCCTCAAGCACATGCACAATTTGAGCGACGA
GGTGCTGTGCGCGCGTTGGGTGGAGAACCCGTATTTCCAGTTCTTCTGCGGCGAGGTGGTGTTCCGCCACGACCTGCCGTTCGACCGCTCCTCGCTGAGC
CGCTGGCGCAAGCGGCTGGGCGAAGAGCAACTCGCGGCGCTGTTGCAGGAGAGCCTTTCGGTGGCGCATCGCGCCGGCGCGCTGCAGACCAAGGATCTGC
AGCGGGTGGTGGTCGACACCACCGTGCAGGAGAAGGCGATCGCGCATCCGACCGACGCCCGGCTCACCCACCGGGCGATCCAGAATCTGGTCGATCTGGC
CAAGCGCGAGGGCGTGGCGCTGCGGCAAAGCTATCTGCGGCTCGCCAAGCGCGCCGCGATCATGGTCGGGCGCTACACCCACGCGCATCAGTTCAAGCGC
GCCCATCGCGCACTGAAATTCCTGCGCACCAGGCTCGGGCGGATCATCCGCGACATCCGCCGCAAGATCGAGGGCAACGCCGCGCTTGAAAATCGCTTCG
CGCCGCTGCTCGATCTCGCGGTTCGGGTGATGCAGCAGGATCATCGCCAGCGCGGCCAGAAGGTCTATGCGCTGCACGCTCCGGAGGTCGAATGCATCGG
CAAGGGCAAGGCGCGAAAGCCATACGAGTTCGGCTGCAAGGTCTCGGTGATCACCCCGGTCACCGCGCCGAAGGGCGGCCAGTTCGTGCTGCACGCCAAG
GCGCTGCACGGCAATCCGTTCGACGGCCACACCCTCGACCCGGTCATCGCCGATCTGCAAACCCAGACCGGCGTCGTGGTTGAACGCATCCATGTCGACA
AGGGCTATCGCGGCCACAACTATCCGAACCGCTTCAAGGTCTGGATCTCCGGCCAAGTCCGCCGCGTCACCAAAATCATTCGCCGCGAGATGAAACGCCG
CGCCGCGGTCGAGCCGGTGATCGGTCATCTCAAGGCCGACCACCGCATGGGCCGCAATTATCTCAAGGGACGCGATGGCGACCGCATCAACCCGGTCCTC
GCCGCCGCCGGCTTCAACTTCCACCTGCTGCTGCGATGGTTCGAGCGGCTTTTGCGCGCCCTATTCGCCGCGCTCTACCGCGTCATCGGATCGATGCAAT
ACGCCTAAATCGGCGCGTGTCCGGGATCTTCACGGACGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1347 bp	448 aa	162	1508	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEGAMSKPRDDRQAEMFRPALEEIIDLGHPLVRLAAEIDWDFLATRFGAVYRPGPGQPPLPTRLIAGLLILKHMHNLSDEVLCARWVENPYFQFFCGEVV
FRHDLPFDRSSLSRWRKRLGEEQLAALLQESLSVAHRAGALQTKDLQRVVVDTTVQEKAIAHPTDARLTHRAIQNLVDLAKREGVALRQSYLRLAKRAAI
MVGRYTHAHQFKRAHRALKFLRTRLGRIIRDIRRKIEGNAALENRFAPLLDLAVRVMQQDHRQRGQKVYALHAPEVECIGKGKARKPYEFGCKVSVITPV
TAPKGGQFVLHAKALHGNPFDGHTLDPVIADLQTQTGVVVERIHVDKGYRGHNYPNRFKVWISGQVRRVTKIIRREMKRRAAVEPVIGHLKADHRMGRNY
LKGRDGDRINPVLAAAGFNFHLLLRWFERLLRALFAALYRVIGSMQYA

Blast result :

Comments

ISRpa8 is 74% aa similar to ISRm13.

References

1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Pelletier,D.A., Kyrpides,N., Anderson,I., Oda,Y., Harwood,C.S. and Richardson,P. (2006) Direct submission GenBank.