ISfinder Mirror Site

ISRsp5

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) NGRIS-8

Accession number	Transposition	Origin	Host
NC_012586	ND	Rhizobium sp.	Rhizobium sp. NGR234 Rhizobium sp. NGR234 plasmid pNGR234b

DNA section

IS Length : 1320 bp

Ends

IR Length : 14

IRL : CAGGCTGCTGAAAAAGCCCTGGCGCTTGGGTTGGATCAATGATTCCCTGC
IRR : CAGGCTGCTGAAAATCGTGTTGCGTTACCGCGAGATACTGCGGTGGGCGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCAAAATGCT	CTAG	AACGACAGAT	4
GCACTGCGCT	CTAA	GCAGCGCGTC	4
CCACAATGCC	CTAA	AGCTCATCGA	4

DNA sequence

CAGGCTGCTGAAAAAGCCCTGGCGCTTGGGTTGGATCAATGATTCCCTGCGGTTGCAACAGAGGGGACTGATTTGCATGCGCGGTGGGGACGACCGGACG
GGATCACTGTTTTCCTATGTCGACCTTGAGGCACGGGTTCCGGCCAGTCACCCGCTTCGGGTGATGCGCGCGCTGGTGAACGCTTCGCTTGTGGCGCTGG
ATGGGTCGTTCGCGGCGCTCTATGCGAAGAGGGGTCGCCCATCGATTGCTCCGGAACGGATGCTGCGGGCAGTGCTGTTGCAGATGCTCTATTCGATCCG
CTCCGAGCGTCAGTTGATGGAACGGCTGGAGTTCGACCTGTTGTTCCGCTGGTTCGTGGGCCTTGGGATCGACGATGCGGTGTGGGACGCCTCGACGTTT
TCGAAGAACCGGGACCGGCTGCTGACGACTAAGATCGCGCAGGGCTTCCTGTCGGCGCTGCTTTCGCAGCCGGCGGTGAAGAAGCTCCTGAGCGCCGAGC
ATTTCTCGGTCGACGGCACGATGTTGAAGGCGTTCGCCTCGATGAAGAGCTTCCGGGCCAAGGATGGCCCAAAGGGCGACAATGACCAGCCACCGGAAGA
CGGCCGCAACGGCGAGCGCGACTTCCGCAAGGAGAAGCGCTCGAACGAGACGCATGCCTCGACGACCGACCCGGACGCGCGGCTTTACCGCAAGGGCAAT
GGTCAGGAGAGCCGGCTGGCCTATCTGGGGCACGCGCTGATGGAGAACCGCAACGGCCTGGCGGTCGCCGGCATGGTGACGCATGCGACCGGCACCGCCG
AGCGGGAAGCGGCCACCGAACTGAGTGCGGACCTTGCCGAAGGCGCGACGCTTTGCGGCGACAAGGGCTACGACGCGGAAGCGTTCGTCGAGGGGCTGAA
GGACCGCAAAATCGTGCCGCATGTGGCGATCAACGGCACGGTGAGCAAGACCGGCAAGGTGCGCAAGACGGCAGTGCCGCCGCAGGTCGCGCAAAGTCCC
GGCTATGCGATCAGCCTGCGCTGCCGCAAGCGGATCGAGGAAATCTTCGGCTGGATCAAGACGACGGCCGGCTTCACCCAACTCAAGGTCCGCGGACTGG
ACAAGGTGAAGGCGGCCTTCACCTTCGCCCTTGCCGCCTACAACATCGTTCGTCTTCCGAAATTGCTCGGCTCGACGGGAGAAGTGTGCCTTGAGGGAGG
CAAATAGGCCAATATGACCGGAAAACCTGCCTTGACCGGCCTGTTTCCTGCCGATACCCGCCAGAAAACCGCGCCCACCGCAGTATCTCGCGGTAACGCA
ACACGATTTTCAGCAGCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1131 bp	376 aa	77	1207	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGGDDRTGSLFSYVDLEARVPASHPLRVMRALVNASLVALDGSFAALYAKRGRPSIAPERMLRAVLLQMLYSIRSERQLMERLEFDLLFRWFVGLGIDD
AVWDASTFSKNRDRLLTTKIAQGFLSALLSQPAVKKLLSAEHFSVDGTMLKAFASMKSFRAKDGPKGDNDQPPEDGRNGERDFRKEKRSNETHASTTDPD
ARLYRKGNGQESRLAYLGHALMENRNGLAVAGMVTHATGTAEREAATELSADLAEGATLCGDKGYDAEAFVEGLKDRKIVPHVAINGTVSKTGKVRKTAV
PPQVAQSPGYAISLRCRKRIEEIFGWIKTTAGFTQLKVRGLDKVKAAFTFALAAYNIVRLPKLLGSTGEVCLEGGK

Blast result :

Comments

References

1] ISfinder annotation (2010)
2] Schmeisser,C., Liesegang,H., Krysciak,D., Bakkou,N., Le Quere,A., Wollherr,A., Heinemeyer,I., Morgenstern,B., Pommerening-Roser,A., Flores,M., Palacios,R., Brenner,S., Gottschalk,G., Schmitz,R.A., Broughton,W.J., Perret,X., Strittmatter,A.W. and Streit,W.R. (2009) Appl. Environ. Microbiol. 75 (12), 4035-4045
3] Streit,W.R., Schmitz,R.A., Perret,X., Staehelin,C., Deakin,W.J., Raasch,C., Liesegang,H. and Broughton,W.J. (2004) J. Bacteriol. 186 (2), 535-542