ISAzo13

  • Family ISAzo13
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Azoarcus sp. EbN1
DNA section
IS Length : 1305 bp

Ends


IR Length : 21/30

IRL : GGACTCGTTAAAAAACAACTTGAACAGTAAGGTCGACGCCGTTATGCTGC
IRR : GGAATTGTTCGCAAATAACCTGAACAATTATTGGGCTCTGGGGCTG

Insertion site


Left flankDirect repeatRight flankDR Length
GCCGCCGAGCACCTACGCCTAGAAAGGCTCACATGG3
GGCGCGCCAGCAGTGCGCTAATTGAGGATACAGGAC3

DNA sequence

GGACTCGTTAAAAAACAACTTGAACAGTAAGGTCGACGCCGTTATGCTGCGCAAATGGCATCTACCGATGATGAGATTGAGCAGCGTTACCAGCTTTTGT
CGAGCCGGCTGGATGAGCGACAGCGTCGGCTGTTTGTAGCGACGGAGGCGAAGGTGTTTGGCCGAGGCGGCATTTCGGCCGTATCGAGGGCGACGGGGGT
ATCGCGCGTAACGATCACGGCTGGCCTGGGTGAGCTGGAATCTGACGCGGTTGCGGAGTCCGCCCCTTTGCCGCCAGGGCGAGTGCGTCGTTTGGGCGGG
GGGCGCAAGAAGCTTGCGCAGACCGATCCCACCCTGCTGTCCGACCTTGAAATGCTGATTGACCCGGTGACACGGGGTGACCCTGAGTCGCCGCTGCGCT
GGACCTGCAAGAGTTTGCGGAAATTGGCCGACGAACTGCGTAGCCAGGGGCATCAAGTGAGCCATGTCGTCGTGGGGGAACTGCTCAAGGCGCAAGGCTA
CAGCCTGCAGGCCAATGCCAAGACGCTTGAAGGGGGCGATCATCCGGATCGCGATGCTCAGTTTATCCAGATCAATGCCCGCGTCGGAACTGCGCTCGAA
GCGGGCGAGCCGGTCATCTCGGTGGACACCAAAAAGAAGGAGCTGGTCGGCGCCTACAAAAATGCCGGTCGGGAATGGGAGAAGGCCGGCCGGCCGGTCG
AAGTCAAGGTGCATGACTTCATTGATCCCGATTTGGGACGTGCCAACCCATACGGCGTCTATGACGTTGGCGCAGACCAGGGGTGGGTCAGTGTAGGGAC
CGATCATGACACCGCGGCCTTTGCAGTGGAGTCGATCCGCCGCTGGTGGTTCGCCATGGGCCAAGCCCTATACCCCGGAGCCCGCGAATTGACGATCACG
GCAGACGGCGGTGGGAGCAACGGCTCTCGAGTGCGTCTGTGGAAGCAGGAATTGCAGACGCTGGCCAACGATCTTGGATTCCCGGTTCGGGTTTGCCATT
TCCCGCCGGGCACCAGCAAGTGGAACAAGATCGAACACCGGTTATTCTCGTTCATCAGCATGAACTGGCGCGGTCGCCCGTTGATCAGCCATGAGGTCAT
CGTCAATCTGATCGGCTCCACAACGACTCGTTCCGGTTTGGCAGTGCGCGCGCAACTCGACACCAACATCTATCCCAAGGGAGTCAAGGTCCCTGACGAC
GCATTCGCCGCTATCAATATCGAACGCGATCCTTTCCATGGCGAATGGAATTATCGAATCAGCCCCAGAGCCCAATAATTGTTCAGGTTATTTGCGAACA
ATTCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1224 bp407 aa551278+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MASTDDEIEQRYQLLSSRLDERQRRLFVATEAKVFGRGGISAVSRATGVSRVTITAGLGELESDAVAESAPLPPGRVRRLGGGRKKLAQTDPTLLSDLEM
LIDPVTRGDPESPLRWTCKSLRKLADELRSQGHQVSHVVVGELLKAQGYSLQANAKTLEGGDHPDRDAQFIQINARVGTALEAGEPVISVDTKKKELVGA
YKNAGREWEKAGRPVEVKVHDFIDPDLGRANPYGVYDVGADQGWVSVGTDHDTAAFAVESIRRWWFAMGQALYPGARELTITADGGGSNGSRVRLWKQEL
QTLANDLGFPVRVCHFPPGTSKWNKIEHRLFSFISMNWRGRPLISHEVIVNLIGSTTTRSGLAVRAQLDTNIYPKGVKVPDDAFAAINIERDPFHGEWNY
RISPRAQ

 

Blast result :
Comments
ISAzo13 is 65% aa similar to ISArch16.
References
1] ISfinder annotation (2008)
2] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503
3] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36