ISfinder Mirror Site

ISRhru4

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007641	ND	Rhodospirillum rubrum	Rhodospirillum rubrum ATCC 11170 plasmid unnamed

DNA section

IS Length : 1411 bp

Ends

IR Length : 15/16

IRL : GAGTGGTGTAACAGCGTGTGCCTGGGGATGATGGCGCAGGGCGCGGAGCG
IRR : GATTGGTGTAACAGCGCCGTCGCCGTTTGACCTCCGGCAGGCTTGGCCGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCTCAAGGTCACGTCCCCCC		CAAGGGACACGATCGTTCAT	0
GAGCGCGATCGTGTCCCTTG		GGGGGGACGTGACCCGATGA	0

DNA sequence

GAGTGGTGTAACAGCGTGTGCCTGGGGATGATGGCGCAGGGCGCGGAGCGCCCGGAGGCATCATCCCCAGGCGGCCCAATTTCTGGGTGGTCATCGACGA
TCTTCGGTAAGGTTTGGTTGCGAACTTCAACCAGACCAAAGGAACCGACGATGACCAATGACATAATGAGCCTCCGCGGGCTGCTGGAAAAGAGCGCCGA
TGCCGATCTGCTGCGCGAGATGATCGGCTTCGCCGCCGAGCGGCTGATGGCGCTGGAGGTCGAAAGCCTGACCGGTGTCGGCCATGGCGAGCGCAATCCC
GATCAGCGCCTCGTTCACCGCAACGGCTACCGCGAGCGCGACTGGGAGACCCGGGCCGGGACGGTGGAGTTGCGCATCCCGCGCCTGCGCAAGGGCAGAT
AGCTGCCCTGCTTCCTGGAACCGCGCCGCATGGCGGAAAAGGCGCTGACGGCGGTCATTCAGGAAGCCTACATCCAGGGCATCTCGACCCGTTCGGTCGA
CGACCTAGTCAAGGCCATGGGCATGAGCGGTATCTCCAAGAGCCAAGTCTCGCGGCTGTGCGCCGAGATCGACGGCAAGGTCGCCACCTTCCTCGACCGC
CCGCTGGAGGGCGACTGGCCCTACATCTGGCTCGACGCCACCTATGTGAAGGTGCGCCAGGACGGCCGCATCGTCTCGGTGGCGGTGATCATCGCGGTGG
GCGCCAACACCGCCGGGCGCCGCGAGGTGCTGGGCATGACCATCGGCCCCAGCGAGGCCGAGACCTTCTGGACCGAGTTCCTGCGCACGCTGGCAAGGCG
TGGCCTGCGCGGCGTCAAGCTGGTGGTGTCCGACGCCCACGAAGGCATCAAGGCCGCCGTCTCCAAGGTGCTGTGCGCCACCTGGCAGCGGTGCCGCGTC
CACTTCATGCGCAACGTGCTTGCCCATGCCGGCAAGTCGGGGCGCCGCGTCGTCTCGGCCTTCATCGGCACCGCTTTTGCCCAGGACACCGCCCAGGCCG
CCAGCGCCCAATGGCGCCAAGTGGCCGACCAGCTCCGCCCCAGATCGCGGAAACTGGCCGAACTGATGGACGATGCCGAAACCGACGTCCTGGCCTACAT
GACTTTTCCGCCCGCCCACCGGACAAAACTACACTCGACCAACCCCATCGAGCGCCTCAACGGCGAGATCAAGCGCCGCACCGACGTGGTCGGCATCTTC
CCCAACGAGGCCGCCATCACCCGCCTGATCGGGGCCATCCTCCTGGAGCAGAACGACGAATGGGCCGTCCAGCGATCCCGCTACATGACTCTAGAATCCG
TCGCTCCCTTGGGCGATGATGCCCTCACTAGGCTGCCGCCCATTGCGGCAGCCTGATCAACCCGGCCAAGCCTGCCGGAGGTCAAACGGCGACGGCGCTG
TTACACCAATC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1206 bp	401 aa	151	1356	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTNDIMSLRGLLEKSADADLLREMIGFAAERLMALEVESLTGVGHGERNPDQRLVHRNGYRERDWETRAGTVELRIPRLRKGR*LPCFLEPRRMAEKALT
AVIQEAYIQGISTRSVDDLVKAMGMSGISKSQVSRLCAEIDGKVATFLDRPLEGDWPYIWLDATYVKVRQDGRIVSVAVIIAVGANTAGRREVLGMTIGP
SEAETFWTEFLRTLARRGLRGVKLVVSDAHEGIKAAVSKVLCATWQRCRVHFMRNVLAHAGKSGRRVVSAFIGTAFAQDTAQAASAQWRQVADQLRPRSR
KLAELMDDAETDVLAYMTFPPAHRTKLHSTNPIERLNGEIKRRTDVVGIFPNEAAITRLIGAILLEQNDEWAVQRSRYMTLESVAPLGDDALTRLPPIAA
A

Blast result :

Comments

ISRhru4 is 88% aa similar to ISMtsp13. There is a STOP codon in frame and the transposase is probably inactive.

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Munk,A.C., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K., Richardson,P., Zhang,Y., Roberts,G., Reslewic,S., Zhou,S. and Schwartz,D.C. (2009) Direct submission GenBank.