ISAmu1

  • Family IS1595
  • Group ISNwi1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010655 ND Akkermansia muciniphila
Akkermansia muciniphila ATCC BAA-835
DNA section
IS Length : 2422 bp

Ends


IR Length : 18/21

IRL : GGGATGATTCCAATGCTGGAAAATCATCTCCCGGGCCTGGAGGCGGTTAT
IRR : GGTATGATTCCAATTTTGGAATGAGCATTAGAGGAATGTACGGATTCCAT

Insertion site


Left flankDirect repeatRight flankDR Length
TGCAAATGGAGTGTTGAATA0

DNA sequence

GGGATGATTCCAATGCTGGAAAATCATCTCCCGGGCCTGGAGGCGGTTATTCTCCCGGAACTCCGGCGTATCCCACACCAGGTTGACATTGGGGACCTCG
GACCAGACAATCAGCCCCGCCTTGTCGCACAGGTCGTAAATATTGGTGGATGCGGGATAGTGGCCCGTTCTAACCCCATCCGCCCCCATATCCGCAATCA
GCCTGATATCCCGCGCCTCATCTTCCGGGGAAAGGGCCCACCCCTTCCCCTTCATATCCTGATGGCGGCTGACCCCCTTGATCTGCATGGTCTTTCCGTT
CAGGACAAAACCTTCCTTGGGATTGACGGAAACCGTCCTCAGGCCCAGCGGCTGCACCAGCTCATCCGTCTGGCCATCCGCCGTCCTGATGGAAGTTTTC
ACCTGGTACAGGTAAGGATTCTTCACTCCGTTCCAGAGTACGGGATTGGCGATTGCCAGCGTTACGGGAACCTCCAGTCTATTCTTTTCCCCGGCGGCGG
CCTTTACCGTCTTCCCGGCTACCTTCCTGCCCTTCATGTCCAGGATTTCCACGGCAACATCCACATCCCCGGCCTTTCCGTCCGAATTGAGAAGGGTCTT
CACTTCCACCTCGGCCTTGGATTTGCTTAAAGACTTTGTGGTGATGAAAACGCCGGGAGAAGCATAAAAAAGAGGATCAATGCAGACACCGTCCGTCTCA
ATCAGTTCAATGGGCCGGTACAGCCCGCCGAACATGGAAAAATCCCCGCGCTGGGGGGCGATATGATTACGCTGCGTGTTGTCCACCAGAACGGAAATCG
TATTGGAGCCTTTTTTCAAAGGGCCTTATATACTTGACTTCGGTTTCTCCGTATGCTATTAATTAGCCATACTAAGGAGGCACCCATGAACGACCGCTTA
ACTATTTCACTTTTTGAGCTTTTTGAAAAATTCCCCAATGAGGAATCAGCTAAAAAGTATTTAGAGGAAAAGCGGTGGGGCGATAAGGTAGTTTGCCCGT
TCTGCGGGAAAAGCGAAAAACAGTATAGAGTAAAGAGGAACGGGGTTGAGGGGTATTTTGAATGCGGGGAGTGCGGGAAAGTTTATACTGTGAGGACTGG
AACTATTTTTGAGAGAAGCCATGTTCCACTTCATAAATGGATTTTTGCCTTCTATTTAGTAGTGACTTCCAGAAAGGGTATTAGCTCCATGCAACTTTCC
AAGGAAATTGGGGTTACGCAAAAAACGGCGTGGTTCATGCTTCAAAGGATTAGGGAAGCATGCAATTCCAACCATGATGACGATGACAATAACGGCTTTC
TATCTGGAATTGTTGAAGCTGATGGGGTGTATATTGGAGGCAAGGAATCCAATAAGCACGAATCTCAGAAATTGAAGGCTGGGCGCGGAATTGTTGGAAA
GACAGCGGTTTTAGGAATGAGAGCCAGAGCAGGAAAGGTTTTAGCAAGGGTTATTCCCGACACTTCAAGAGATACAGTTCACCAAGTTTTGAATAAGTAT
TTGAGCCGGGATTCCGTTCTGGTTACTGATGAACATGCTTCTTACAAAGAATCACAGTTCAATCATCAGGTGGTGAATCATAGTGCCAAGCAGTATGTTG
ACGGAATGGCCCATACTAACGGCATCGAGAGCGTATGGGCGGTTTTGAAGAGAGGATTCTATGGAATCTACCATTCATTCAGCACAAAACATTTACAGAG
ATATGTAGATGAATTTGCGTATCGGTTGAATGAGGGAAATGTGAAAATCCACACATGGGTAAGATTGGATTCTCTTATGGCTAAAGCATTTTCGACGAGA
ATTACTTACGCCGAATTGAAGAATTGACCGCCACCTTGCGAGCAAGGCGACGGCCTAAGTCATGCAAATCAAAGAATCGCCCCCTTCCCCGAAAGGAAGG
AGGCCAGTTAATTCACCGCCGAAAGTTAGAATGACCAGCTAAAGCCAACTGTGATAAAGTGGTATGGGTCAACATCTGACCAGTATTTCGTTATTTCATA
TCCAATAACGATGTCTGTTTTTTCTGCAACAGCAAATTTGAAACCGATTAATGCCGAGTAAGTTCCACTAGAATCAGACTCAGATACTTTTCCTCCACCT
CCAGAGAGTTCCTGTTTATAATAATGAAGGCCTATTTTGCCGCCTAAATAGAAAGTGGCAGAATCAGATAAGGGGACATTAAAAGAATATCCAGCGGTAA
ATGGGAATACCTGTTGTTTCCATTTCAACTCCCCTTCGTAGGTTTTAGAACCTGTAAGCGCGCCGAGATTGAAAGATAATTCATGGAAAACAGAACCCGC
TGGAATGTTAGCGTGAAGTCCTAATTGGGCACCGTACAGGTCTGGCATATCTATTCCATAATCATTCCCCGAATGGAATCCGTACATTCCTCTAATGCTC
ATTCCAAAATTGGAATCATACC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
942 bp313 aa8861827+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNDRLTISLFELFEKFPNEESAKKYLEEKRWGDKVVCPFCGKSEKQYRVKRNGVEGYFECGECGKVYTVRTGTIFERSHVPLHKWIFAFYLVVTSRKGIS
SMQLSKEIGVTQKTAWFMLQRIREACNSNHDDDDNNGFLSGIVEADGVYIGGKESNKHESQKLKAGRGIVGKTAVLGMRARAGKVLARVIPDTSRDTVHQ
VLNKYLSRDSVLVTDEHASYKESQFNHQVVNHSAKQYVDGMAHTNGIESVWAVLKRGFYGIYHSFSTKHLQRYVDEFAYRLNEGNVKIHTWVRLDSLMAK
AFSTRITYAELKN

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
474 bp157 aa23991926+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

 

Blast result :
Comments
ISAmu1 is 63% aa similar to ISRhba1. The second ORF is a passenger gene annotated as hypothetical protein. Before the transposase, there is a pseudogene annotated as a possible beta-galactosidase.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Lang,D., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Smidt,H., Derrien,M., Plugge,C.M., Zoetendal,E.G., de Vos,W.M. and Richardson,P. (2008) Direct submission GenBank.