ISPrsp2
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_GG698740 | ND | Parabacteroides sp. | Parabacteroides sp. D13 |
DNA section
IS Length : 1021 bp
Ends
IR Length : 21/22
IRL : ACCTGTTGGCGGAATTAAATTATAAAAGATTTATGTTTAAAAAGTTGTGC
IRR : ACCTGTTGGCGGAATTAAAATAGCGCATATTTAACTCTGCCAATAGGCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AATGAAGTTA | CATGCTAATA | 0 |
DNA sequence
ACCTGTTGGCGGAATTAAATTATAAAAGATTTATGTTTAAAAAGTTGTGCATATTGTTGATATTTAGTAAATTAAAGGTGACTAAACTTTTAATAGACAA
ATATCGTATGCACAACTTATATGCAATATTCGCAAAATTACTGAACATATGCAAGCAAATTGCCGGCAATTTAGTCAATGAATCCGGGAATGTACCAAGA
CGAGGAGTCGTCCCTAAATTCTCAGACCTTGAAGTAGTGGCTTTGAACATGGCATCAGAGGCTGTTGGTATTGACAGTGAGTCGCTGTTGTTTGCAAAGC
TACAGGAATATAGGGTTGAAATACCCAACCTTATTTCCCGCCGACAATACAATGACAGGCGTAAAATAACTTCCTCCCTATGTAATGCAATCCGAGAAAG
AATGGTTTCTAAAATGGATGGTGGTGAAGACTATTTCTGTATTGATTCGAAACCGATAGAAGTATGTCGTATTGCCCGTTCCAAACGTTGCAGTATGGGA
AAGAAGGATTTTAGAAAAGCACCTGGGGTAGGATACTGCGCATCACAAAGCATGTATTATTATGGGTATAAACTCCATGCAGTCTGCTGGTTAAGTGGTA
TCATCCATTCCTTTGACCTCACTAAGGCAAGTGTGCATGACATTCATTACCTGAAGGATGTGAAAGTGGATTATAGTAATTGTACAGTCATAGGGGACAG
AGGATATATAAGTGCCCAAGTGCAATTGGATTTGTTTGAAACTGCCAATATCAGATTGGAGGTACCATACAGATGTAATCAAAAAGAATGGAAGCCAACA
TTCCCAGCTTTTGCCAAAGCGAGAAAAAGAATTGAAACCCTATTCTCGCAATTGTGTGACCAGTTTATGATTATAAGGAATTATGCGAAAGATACAGATG
GATTGTTTGCCCGGATTATTGGGAAGATTAGCGCACTTACAATCCTTCAATATATTAACTACAAAAATGAAAAGCCTATTGGCAGAGTTAAATATGCGCT
ATTTTAATTCCGCCAACAGGT
ATATCGTATGCACAACTTATATGCAATATTCGCAAAATTACTGAACATATGCAAGCAAATTGCCGGCAATTTAGTCAATGAATCCGGGAATGTACCAAGA
CGAGGAGTCGTCCCTAAATTCTCAGACCTTGAAGTAGTGGCTTTGAACATGGCATCAGAGGCTGTTGGTATTGACAGTGAGTCGCTGTTGTTTGCAAAGC
TACAGGAATATAGGGTTGAAATACCCAACCTTATTTCCCGCCGACAATACAATGACAGGCGTAAAATAACTTCCTCCCTATGTAATGCAATCCGAGAAAG
AATGGTTTCTAAAATGGATGGTGGTGAAGACTATTTCTGTATTGATTCGAAACCGATAGAAGTATGTCGTATTGCCCGTTCCAAACGTTGCAGTATGGGA
AAGAAGGATTTTAGAAAAGCACCTGGGGTAGGATACTGCGCATCACAAAGCATGTATTATTATGGGTATAAACTCCATGCAGTCTGCTGGTTAAGTGGTA
TCATCCATTCCTTTGACCTCACTAAGGCAAGTGTGCATGACATTCATTACCTGAAGGATGTGAAAGTGGATTATAGTAATTGTACAGTCATAGGGGACAG
AGGATATATAAGTGCCCAAGTGCAATTGGATTTGTTTGAAACTGCCAATATCAGATTGGAGGTACCATACAGATGTAATCAAAAAGAATGGAAGCCAACA
TTCCCAGCTTTTGCCAAAGCGAGAAAAAGAATTGAAACCCTATTCTCGCAATTGTGTGACCAGTTTATGATTATAAGGAATTATGCGAAAGATACAGATG
GATTGTTTGCCCGGATTATTGGGAAGATTAGCGCACTTACAATCCTTCAATATATTAACTACAAAAATGAAAAGCCTATTGGCAGAGTTAAATATGCGCT
ATTTTAATTCCGCCAACAGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 975 bp | 324 aa | 33 | 1007 | + | No |
Chemistry : DDE
ORF sequence :
MFKKLCILLIFSKLKVTKLLIDKYRMHNLYAIFAKLLNICKQIAGNLVNESGNVPRRGVVPKFSDLEVVALNMASEAVGIDSESLLFAKLQEYRVEIPNL
ISRRQYNDRRKITSSLCNAIRERMVSKMDGGEDYFCIDSKPIEVCRIARSKRCSMGKKDFRKAPGVGYCASQSMYYYGYKLHAVCWLSGIIHSFDLTKAS
VHDIHYLKDVKVDYSNCTVIGDRGYISAQVQLDLFETANIRLEVPYRCNQKEWKPTFPAFAKARKRIETLFSQLCDQFMIIRNYAKDTDGLFARIIGKIS
ALTILQYINYKNEKPIGRVKYALF
ISRRQYNDRRKITSSLCNAIRERMVSKMDGGEDYFCIDSKPIEVCRIARSKRCSMGKKDFRKAPGVGYCASQSMYYYGYKLHAVCWLSGIIHSFDLTKAS
VHDIHYLKDVKVDYSNCTVIGDRGYISAQVQLDLFETANIRLEVPYRCNQKEWKPTFPAFAKARKRIETLFSQLCDQFMIIRNYAKDTDGLFARIIGKIS
ALTILQYINYKNEKPIGRVKYALF
Blast result :
Comments
ISPrsp2 is 82% aa similar to IS1187. ISPrsp2 was reconstructed in silico by deletion of ISPrsp1 and of one of the direct repeat generated by ISPrsp1 insertion.
References
1] ISfinder annotation (2009)
2] Ward,D., Young,S.K., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Hepburn,T., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Walk,T., White,J., Yandava,C., Allen-Vercoe,E., Strauss,J., Sibley,C., White,A., Ambrose,C., Lander,E., Nusbaum,C., Galagan,J. and Birren,B. (2009) Direct submission GenBank.
2] Ward,D., Young,S.K., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Hepburn,T., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Walk,T., White,J., Yandava,C., Allen-Vercoe,E., Strauss,J., Sibley,C., White,A., Ambrose,C., Lander,E., Nusbaum,C., Galagan,J. and Birren,B. (2009) Direct submission GenBank.