ISMno18
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011887 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 plasmid pMNOD02 |
DNA section
IS Length : 864 bp
Ends
IR Length : 16/19
IRL : GGGCGCAGTGACCAATTAAGGAAGTCAGGCTGAGGCAAGGTCGGCCTGAT
IRR : GGGTGTAGTGACAAATTAACTACCGAAGCCAGATTGCGATGGCGGCGAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTGTCATGCCA | TAGTGCGGGATGAT | 0 |
DNA sequence
GGGCGCAGTGACCAATTAAGGAAGTCAGGCTGAGGCAAGGTCGGCCTGATGGTGTGGCGCCGGATCGGGAGATGGAGCATGACCGGCGCTGAGGATGGCC
TGCGAGACGACCAGTGGGAGCGGGTGAAGGATCTGCTGCCTGGCGGGCGCAAGGGCAAGCGCGGTCCCCGTTTGCAGAACCGCTTGTTTCTCGATGCGAT
CTTGTGGTTTGCCCGGTCTGGCGGGCGCTGGAAGGATTTGCCGGAGCGGTTTGGCCAATCTGATACGATCAAGCGGCGCTACTATCGCTTGGTTGATCGA
GGTGTTTTCGATCAGATCTTGGACCGTCTCGCCCAAGACGCGGATCTCGAGTGGCTGATGATTGATTCGACGATTGTTCGTGCCCATCATCGGGCGGCGG
GAGCCCGCCTGACAAAAGGGGGGCGGCTGCCCAGGGGTTGGGCCGGTCTCGCGGAGGCCTGAGCACCACGATCCACGCGGCGGTGGATGCTCATGGCAAT
CCCGTGCGGCTGATCGGCGGCCCCGGCCAGGAGCATGACCTGGCCAAGGCCGGAGCCTTGATCGCGGGCTTCCCGGCTGAGTTCACGCTGGCCGACAAGG
GGTACGATGGCGATCCCCTCTGCGACGCGATCACGGCGCAAGGCAGCGAGGTGGTGATCCCGCCGCGCCGCGGCCGCACCCATCCTCGCTCCTATGACAA
GGATTTGTACAAAGAACGCAACGTGATTGAGCGGTTTTTCGCCCGGCTCAAGGATTTTCGACGCGTCGCGACCCGCTACGACAAGCTGCTGAAAAACTAC
ATGGGCTTCGTGACGCTCGCCGCCATCGCAATCTGGCTTCGGTAGTTAATTTGTCACTACACCC
TGCGAGACGACCAGTGGGAGCGGGTGAAGGATCTGCTGCCTGGCGGGCGCAAGGGCAAGCGCGGTCCCCGTTTGCAGAACCGCTTGTTTCTCGATGCGAT
CTTGTGGTTTGCCCGGTCTGGCGGGCGCTGGAAGGATTTGCCGGAGCGGTTTGGCCAATCTGATACGATCAAGCGGCGCTACTATCGCTTGGTTGATCGA
GGTGTTTTCGATCAGATCTTGGACCGTCTCGCCCAAGACGCGGATCTCGAGTGGCTGATGATTGATTCGACGATTGTTCGTGCCCATCATCGGGCGGCGG
GAGCCCGCCTGACAAAAGGGGGGCGGCTGCCCAGGGGTTGGGCCGGTCTCGCGGAGGCCTGAGCACCACGATCCACGCGGCGGTGGATGCTCATGGCAAT
CCCGTGCGGCTGATCGGCGGCCCCGGCCAGGAGCATGACCTGGCCAAGGCCGGAGCCTTGATCGCGGGCTTCCCGGCTGAGTTCACGCTGGCCGACAAGG
GGTACGATGGCGATCCCCTCTGCGACGCGATCACGGCGCAAGGCAGCGAGGTGGTGATCCCGCCGCGCCGCGGCCGCACCCATCCTCGCTCCTATGACAA
GGATTTGTACAAAGAACGCAACGTGATTGAGCGGTTTTTCGCCCGGCTCAAGGATTTTCGACGCGTCGCGACCCGCTACGACAAGCTGCTGAAAAACTAC
ATGGGCTTCGTGACGCTCGCCGCCATCGCAATCTGGCTTCGGTAGTTAATTTGTCACTACACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 79 | 462 | + | No |
Description : First part of the transposase
ORF sequence :
MTGAEDGLRDDQWERVKDLLPGGRKGKRGPRLQNRLFLDAILWFARSGGRWKDLPERFGQSDTIKRRYYRLVDRGVFDQILDRLAQDADLEWLMIDSTIV
RAHHRAAGARLTKGGRLPRGWAGLAEA
RAHHRAAGARLTKGGRLPRGWAGLAEA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 480 bp | 159 aa | 366 | 845 | + | No |
Description : Second part of the transposase
ORF sequence :
FDDCSCPSSGGGSPPDKRGAAAQGLGRSRGGLSTTIHAAVDAHGNPVRLIGGPGQEHDLAKAGALIAGFPAEFTLADKGYDGDPLCDAITAQGSEVVIPP
RRGRTHPRSYDKDLYKERNVIERFFARLKDFRRVATRYDKLLKNYMGFVTLAAIAIWLR
RRGRTHPRSYDKDLYKERNVIERFFARLKDFRRVATRYDKLLKNYMGFVTLAAIAIWLR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 767 bp | 255 aa | 79 | 845 | + | Yes |
Chemistry : DDE
ORF sequence :
MTGAEDGLRDDQWERVKDLLPGGRKGKRGPRLQNRLFLDAILWFARSGGRWKDLPERFGQSDTIKRRYYRLVDRGVFDQILDRLAQDADLEWLMIDSTIV
RAHHRAAGARLTKGGAAAQGLGRSRGGLSTTIHAAVDAHGNPVRLIGGPGQEHDLAKAGALIAGFPAEFTLADKGYDGDPLCDAITAQGSEVVIPPRRGR
THPRSYDKDLYKERNVIERFFARLKDFRRVATRYDKLLKNYMGFVTLAAIAIWLR
RAHHRAAGARLTKGGAAAQGLGRSRGGLSTTIHAAVDAHGNPVRLIGGPGQEHDLAKAGALIAGFPAEFTLADKGYDGDPLCDAITAQGSEVVIPPRRGR
THPRSYDKDLYKERNVIERFFARLKDFRRVATRYDKLLKNYMGFVTLAAIAIWLR
Blast result :
Comments
ISMno18 is 75% aa similar to ISCc1. The third ORF is the transposase reconstructed in silico by possible -1 frameshift.
References
1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.