ISMno8
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011894 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 Methylobacterium nodulans ORS 2060 plasmid pMNOD02 |
DNA section
IS Length : 1466 bp
Ends
IR Length : 16/19
IRL : GAGCCTGTCCGAGTAAGCGTCTCGACGGCGGGGCGCGGAGGTGATTCACT
IRR : GAGCCCGTCCGAGTAACGGGTTGATGCGTTGGGATTGAGGTTGAGATCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGTCTTGAG | CTAG | ATAGAAACCG | 4 |
| GTTGTCACGG | CTAG | TTGTTGACAC | 4 |
| GAGGCGACAG | CTAG | TCAGGATGGG | 4 |
| GACTGCACAG | CTAG | TCATCCTCGC | 4 |
| TGAGGAACGG | CTAG | GAGTGGGACG | 4 |
DNA sequence
GAGCCTGTCCGAGTAAGCGTCTCGACGGCGGGGCGCGGAGGTGATTCACTCGGCCCATGGCCAAGGTGTTTCGCTCCTGGGATGTCGATCAAGGCTGGCT
GCTGCCGCCCTCGTTGCATGAGTTCGTGCCGCCCGGGCATATGGCGCATTTCGTGCGCGACACGGTGCGCGAGGCGCTCGATCTGTCAGCCATCCTCGAC
ACCTATACCGAGGAGCGCGGCTATCCACCCTACCATCCCGGCATGATGGTGGCGCTCCTGCTCTACGGCTACAGCCGCGGCCTGTACTCCTCGCGCCAAC
TCGCCCGCGCCTGCGAGGAGCGGGTCGACGTTATGGCGGTGACCGGCCTGAACCGGCCCGACTTCCGCACCATCGCCGAGTTCCGCAAGCGTCATCTCGC
GGCGCTGTCGGACCTGTTCGTGCAGGTGCTGCGGCTGTGCCAGGCGGCCGGGCTGGTGGGCTTTGCTCATGTGGCGGTGGACGGGACCAAGCTGAAGGCC
AATGCCTCGCGCCACAAAGCGATGAGCTACGGCCGGATGAAGACGACCGAAGCGGCCTTGGCGGCCGAGGTCGAGGCGTGGCTTGCCCAAGCTGCCGAGA
TGGATGCCGCTGAGGACCGCGCGCACGGAATGGATCGGCGCGGCGACGAGACGCCGGACTGGATGGCCGACAAGCAGCGGCGGCTTGCTGCGATCCGCGC
CGCCAAGGCCGCCCTGGAAGCCGAGGCCGCCGATCCGCCCGCCCCTGAGGACGAGAGCGGGCCGGGCGCCTCGTCGGGCATGCGTTGGCAGGGGCGGCCC
CTGCGCGGCGAGGACGGCGGCCCACCCGACCGGGCACAGCGCAACTTCACCGAGCCGGACAGCCGGATCCTGCCCACCCGCGACGGGTTCGTGCAGGGCT
ACAATGGCCAGATCGCGGTCGATGCGGCGCATCAGGTGATCGTGGCGCAGCGGCTCGTGACCAACGCAGCCGACTATCGTGCCCTGGTGCCGCTGATCGA
CGACGTCTCTGCTCATCTCGGACGCAAGCCGCGCGAGGTCTCGGGCGATGCCGGTTTTGCCACCGAGGCGAACCTGACGGCGCTCCAGGCGCGTCGGATC
CGGGGCTACCTGCCGCCCGGCCGAGCGCGTCACGGCGAGGCCCATGCGGCTGGACGGCGCAAGCTGACGAAGATGCCGCTGATGAGCGCGATGGCTCAGC
GGCTGAAGCGGGCTGGGCGCCGGAGCCGCTACCGCCTGAGAAAGCAGGTGGTCGAGCCGGTCTTCGGGCAGATGAAGCAGGCCAGAGGTTTTCGGCAGTT
CCTGCTGCGCGGTCTTGAGCAGGTCCGAGGCGAGTGGGCGATGATCTGCACCGCCCATAACCTCCTGAAGCTGGCGCAGGCCGCGCGCTAAGCCTGTGGC
GCGGAATCCATCCGCCCAGATCTCAACCTCAATCCCAACGCATCAACCCGTTACTCGGACGGGCTC
GCTGCCGCCCTCGTTGCATGAGTTCGTGCCGCCCGGGCATATGGCGCATTTCGTGCGCGACACGGTGCGCGAGGCGCTCGATCTGTCAGCCATCCTCGAC
ACCTATACCGAGGAGCGCGGCTATCCACCCTACCATCCCGGCATGATGGTGGCGCTCCTGCTCTACGGCTACAGCCGCGGCCTGTACTCCTCGCGCCAAC
TCGCCCGCGCCTGCGAGGAGCGGGTCGACGTTATGGCGGTGACCGGCCTGAACCGGCCCGACTTCCGCACCATCGCCGAGTTCCGCAAGCGTCATCTCGC
GGCGCTGTCGGACCTGTTCGTGCAGGTGCTGCGGCTGTGCCAGGCGGCCGGGCTGGTGGGCTTTGCTCATGTGGCGGTGGACGGGACCAAGCTGAAGGCC
AATGCCTCGCGCCACAAAGCGATGAGCTACGGCCGGATGAAGACGACCGAAGCGGCCTTGGCGGCCGAGGTCGAGGCGTGGCTTGCCCAAGCTGCCGAGA
TGGATGCCGCTGAGGACCGCGCGCACGGAATGGATCGGCGCGGCGACGAGACGCCGGACTGGATGGCCGACAAGCAGCGGCGGCTTGCTGCGATCCGCGC
CGCCAAGGCCGCCCTGGAAGCCGAGGCCGCCGATCCGCCCGCCCCTGAGGACGAGAGCGGGCCGGGCGCCTCGTCGGGCATGCGTTGGCAGGGGCGGCCC
CTGCGCGGCGAGGACGGCGGCCCACCCGACCGGGCACAGCGCAACTTCACCGAGCCGGACAGCCGGATCCTGCCCACCCGCGACGGGTTCGTGCAGGGCT
ACAATGGCCAGATCGCGGTCGATGCGGCGCATCAGGTGATCGTGGCGCAGCGGCTCGTGACCAACGCAGCCGACTATCGTGCCCTGGTGCCGCTGATCGA
CGACGTCTCTGCTCATCTCGGACGCAAGCCGCGCGAGGTCTCGGGCGATGCCGGTTTTGCCACCGAGGCGAACCTGACGGCGCTCCAGGCGCGTCGGATC
CGGGGCTACCTGCCGCCCGGCCGAGCGCGTCACGGCGAGGCCCATGCGGCTGGACGGCGCAAGCTGACGAAGATGCCGCTGATGAGCGCGATGGCTCAGC
GGCTGAAGCGGGCTGGGCGCCGGAGCCGCTACCGCCTGAGAAAGCAGGTGGTCGAGCCGGTCTTCGGGCAGATGAAGCAGGCCAGAGGTTTTCGGCAGTT
CCTGCTGCGCGGTCTTGAGCAGGTCCGAGGCGAGTGGGCGATGATCTGCACCGCCCATAACCTCCTGAAGCTGGCGCAGGCCGCGCGCTAAGCCTGTGGC
GCGGAATCCATCCGCCCAGATCTCAACCTCAATCCCAACGCATCAACCCGTTACTCGGACGGGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 57 | 1391 | + | No |
Chemistry : DDE
ORF sequence :
MAKVFRSWDVDQGWLLPPSLHEFVPPGHMAHFVRDTVREALDLSAILDTYTEERGYPPYHPGMMVALLLYGYSRGLYSSRQLARACEERVDVMAVTGLNR
PDFRTIAEFRKRHLAALSDLFVQVLRLCQAAGLVGFAHVAVDGTKLKANASRHKAMSYGRMKTTEAALAAEVEAWLAQAAEMDAAEDRAHGMDRRGDETP
DWMADKQRRLAAIRAAKAALEAEAADPPAPEDESGPGASSGMRWQGRPLRGEDGGPPDRAQRNFTEPDSRILPTRDGFVQGYNGQIAVDAAHQVIVAQRL
VTNAADYRALVPLIDDVSAHLGRKPREVSGDAGFATEANLTALQARRIRGYLPPGRARHGEAHAAGRRKLTKMPLMSAMAQRLKRAGRRSRYRLRKQVVE
PVFGQMKQARGFRQFLLRGLEQVRGEWAMICTAHNLLKLAQAAR
PDFRTIAEFRKRHLAALSDLFVQVLRLCQAAGLVGFAHVAVDGTKLKANASRHKAMSYGRMKTTEAALAAEVEAWLAQAAEMDAAEDRAHGMDRRGDETP
DWMADKQRRLAAIRAAKAALEAEAADPPAPEDESGPGASSGMRWQGRPLRGEDGGPPDRAQRNFTEPDSRILPTRDGFVQGYNGQIAVDAAHQVIVAQRL
VTNAADYRALVPLIDDVSAHLGRKPREVSGDAGFATEANLTALQARRIRGYLPPGRARHGEAHAAGRRKLTKMPLMSAMAQRLKRAGRRSRYRLRKQVVE
PVFGQMKQARGFRQFLLRGLEQVRGEWAMICTAHNLLKLAQAAR
Blast result :
Comments
ISMno8 is 95% aa similar to ISMex36.
References
1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.