ISfinder Mirror Site

ISMno11

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_011894	ND	Methylobacterium nodulans	Methylobacterium nodulans ORS 2060 Methylobacterium nodulans ORS 2060 plasmid pMNOD02 Methylobacterium nodulans ORS 2060 plasmid pMNOD01

DNA section

IS Length : 1058 bp

Ends

IR Length : 18/25

IRL : GAGCGGGGTCCGAGCGGGTTGAAACCGTGGTGATGGCAACGCGTTGGCCG
IRR : GAGCCGCTCCCGATCAGGTTGAAGCGTAAGCATCATCCTCGTATCCAGCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGCCAGGCTC	TA	GCACAGACCG	2
GCTCGGTCTC	TA	GGTGCTCCTC	2
GGGAGCGCTC	TA	GACCCGGCAA	2
GGAGTTGGGC	TA	GGCCAGAGGT	2
TCAGCGCCGC	TA	GCCGCGGCAG	2
CCGCGATCAC	TA	GGCCGGCCAC	2
AGCGGCGTCC	TA	GCTCGCGCCG	2
GGATGGAAGC	TA	GCCCACGCTC	2
CCTCCGAGGC	TA	AGGGCAGCAG	2
TTCCGCGCTC	TA	GCGCAGCAGA	2
CACAGATCAC	TA	GAGAGGATGT	2
TTTTGACCAC	TA	GAGCATTTTC	2

DNA sequence

GAGCGGGGTCCGAGCGGGTTGAAACCGTGGTGATGGCAACGCGTTGGCCGGTCCCCGGGGGGGCGGCTCAACGGAGGTGGCCCATGCCCTCAGCTTTGTC
TGTCGACCTGCGCCAGCGCGTCGTCTCGGCCGTTGCAGAAGGCGCCTCCTGCCATCAGGCCGCCGCGCGCTTCGGGGTCAGCGTGGCCAGCGTCAGCCGC
TGGTCCAGGCAGCAGGAGGGCCAGGGAGATGTCACGCCCAAGCCCATGGGCGGCGACCAGCGCTCGCAGCGCATCGAGGCTCATGCCGAGCTCATCCTGC
AGACCTACCAAGCCCACCCGCAGAGCTTCCTGCATGAGCTCTGCGAGACGCTCCAGGAGCAAGGCGTTCCGGTCAGCCGCAGTAGCCTGTCGCGCTTCTT
CGCCCGGCACCGCATCACGCGGAAAAAAGGGCGACGTACGCAGCTGAGCAGGAGCGGGAGGATGTAAAGGCGGCTCGTGAGGCGTGGTTTGCCGGCCAGC
TTGAGCTGGATCCGGAGCGACTGGTGTTCCTGGATGAGACCGCCGCCACCACCAGCATGGTCCGCCGCTACGGCTGGGCCCCGCGCGGCGAGCGTTGCCG
CCTCGCGGCACCCGCAGGGCACTGGAAAACCACCACTGTGATTGCCGGGCTGCGCACGAGCGGGCCTGACGCGATCGCTCTGCTGGACGGCCCTGTGACG
GGCGAACGCTTCCGCGCCTACGTGACCGACACCCTGGTCCCCACCCTGAGACCCGGCGACACCGTGATCCTGGACAATCTGGGCGCTCACAAGGTGGCCG
GCGTGCGCGAGGCGATCGCGGCAACCGGGGCCCGGCTGCTTTATCTTCCTCCGTACTCACCTGAGTTCAACCCGATTGAGCAGGCCTTCGCCAAGCTGAA
AGCGCTGTTGCGCAGGGCGGCGGCCCGCAGTGTCAGTGAACTCTGGGCGGCGATCCACCAAGCCTTCAAATGCTTCTCGCCGGCTGAGTGCCGCAACTAC
TTCACAGCTGCTGGATACGAGGATGATGCTTACGCTTCAACCTGATCGGGAGCGGCTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
384 bp	127 aa	84	467	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPSALSVDLRQRVVSAVAEGASCHQAAARFGVSVASVSRWSRQQEGQGDVTPKPMGGDQRSQRIEAHAELILQTYQAHPQSFLHELCETLQEQGVPVSRS
SLSRFFARHRITRKKGRRTQLSRSGRM

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
660 bp	219 aa	386	1045	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PVALLRPAPHHAEKRATYAAEQEREDVKAAREAWFAGQLELDPERLVFLDETAATTSMVRRYGWAPRGERCRLAAPAGHWKTTTVIAGLRTSGPDAIALL
DGPVTGERFRAYVTDTLVPTLRPGDTVILDNLGAHKVAGVREAIAATGARLLYLPPYSPEFNPIEQAFAKLKALLRRAAARSVSELWAAIHQAFKCFSPA
ECRNYFTAAGYEDDAYAST

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
962 bp	320 aa	84	1045	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPSALSVDLRQRVVSAVAEGASCHQAAARFGVSVASVSRWSRQQEGQGDVTPKPMGGDQRSQRIEAHAELILQTYQAHPQSFLHELCETLQEQGVPVSRS
SLSRFFARHRITRKKRATYAAEQEREDVKAAREAWFAGQLELDPERLVFLDETAATTSMVRRYGWAPRGERCRLAAPAGHWKTTTVIAGLRTSGPDAIAL
LDGPVTGERFRAYVTDTLVPTLRPGDTVILDNLGAHKVAGVREAIAATGARLLYLPPYSPEFNPIEQAFAKLKALLRRAAARSVSELWAAIHQAFKCFSP
AECRNYFTAAGYEDDAYAST

Blast result :

Comments

ISMno11 is 80% aa similar to ISMex30. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.