ISfinder Mirror Site

ISSwo2

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008346	ND	Syntrophomonas wolfei	Syntrophomonas wolfei subsp. wolfei Goettingen

DNA section

IS Length : 2446 bp

Ends

IR Length : 19/25

IRL : GTAAGCGTCAAAAACTTCACACATACCAGTGTCCCAGTAAAGATGAGACA
IRR : GTAACCGTCAAATAGCGCACACCTAGCCCTCAGGAACTACTTGAGGTAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGATACAAAC	GTATATTC	CGCATCACCC	8
CAAGAGCAAC		GTTGTCAGTC	0

DNA sequence

GTAAGCGTCAAAAACTTCACACATACCAGTGTCCCAGTAAAGATGAGACAATAAACCTGAACAAAAAACAAGGAGATGTTCAGGAATGGTCAATAACGAT
CTGCGCAGCCTGTGGGAACAGCGTCTGGCAGACTATGAAACCAGCGGTAAAAGCATAGCTACCTGGTGTAGGGAACATTCAATTAGGAACAACCAGTTCT
ATTACTGGCGTAAAAAACTGCGTATGGACCAGGTTGAAAATAACCAACCAGTTAAATGGCTTCCCCTGGAAGTAGAGCAAGCAAACCTTGCTCCCGGTTC
CATAGGCGTTCATGTTGGGCAGGCAACAGTTGAGATTAAACCAGGCTTTGATCCCCACCTGTTGCGCCAAATCGTAAAGGTTCTACAAACCATATGATCA
GTGAAATATCAAACCGACTGGTTTACCTGGCCTGCGGTAGCACTGATCTTAGAAAATCGATTGACGGATTGGCAGTACTGGTTAAAGAATCATTCCATTT
AGATCCCTTTAGCCCCTGCCTATTTGTATTTTGTAATCGTAAACGTGACAAACTCAAAATTCTACAGTGGGAGCATAACGGGTTCTGGCTGCATTACCGG
CGTCTGGAGCGGGGCAAATTTGACTGGCCCACCGCCCATACCGATGTGGTCAGTATCAGCTACCGTGAATTCCGTTGGCTCCTGGATGGCTTGTCACTAA
AACAAAATCAGGCTCATAAAGCAGTTAAACAAAGAGTTATTCTTTAACAGAAAAACAGCTCTAAGCCCGGATATTCCGGCATTTTCGTGGTGCAATAATA
CCGCTTTTATGGTATAATTTATCTATGAACATGCAAGATAATTCGGCTCAGATAATTGAACAACTTAATGATAAAATCCTTATGCAGGAACAACAGATTG
CTGAACTTAATGCCAAGGTAAAGTGGTATGAGGAGCAATATCGCTTAAGTCGCCAAAAACAGTTTGGTACTTCCAGTGAGAAAACTACTCCCGAACAGAT
TAATCTGTTTAATGAAGCTGAAGATATTGTTGACCCCGAAATTAAAGAACCAGATATCGAAACCATTACCTATGAGCGTAAGAAAAAGCAGCCTGGTCAA
AAAGCTGATAAGCTTAAAGACCTTCCGGTTGAAGTAATTGAATACCGACTTTTAGAACATGAGCAAGTCTGCCCCTGCTGCCAGGGTTCCCTTCATGAAA
TGAGTACCCAGATCAGACAGGAGATAAAGGTAATACCTGCTCAGGTAAAAGTAGTACAGCATGTTCAATACATATATTCCTGCCGCCAGTGTGAGAAAGA
AAATATTACGACACCGATAATCAAAGCACAAATGCCTAACCCCATATTACCAGGCAGTTTGGCATCCCCCTCCATACTGGCATATATTATGGATCAAAAA
TATACCAACAGTATGCCGTTATATCGTCAGGAACAGCAGTTATCCCGTCTGGGAATAGAGCTGTCCCGCCAGACCATGGCAAACTGGGTTTTAAACGTTG
CCGACCCCTGGTTAAAGATAATTTACGACCGCCTGCATGTGGAGTTGCTTGATAGAGATATCCTGCACGCGGACGAAACAACCTTGCAGGTATTAAAAGA
ACCGGGGCGTTCAGCCGAAACTAAATCCTATATGTGGTTATATCGTACCGGAAGAGATGGACCACCAATTGTCCTGTACGAATATCAGACCACCAGAGCC
AGTAAACATCCGGACCGATTTCTGTCTGGTTTTAAAGGATATCTACAAACTGATGGTTACAGTGCCTATGGTAAACTGACCGGCATAACGCTGGTCGGCT
GCTGGGCGCATGCACGCAGAAAATTCACCGAAGCCCTTAAAGCATTGCCGGCTGCCCAAAAAGATAAACCGGTAGCAGCCAGTGTGGGGTTGGAATACTG
CAATAGATTATTTGCTATTGAACGTCAGTTAAAAGATGTATCTGATAAAGAACGTTATGATAAACGGTTGGAAAAAAGCAAACCGTTACTTGACGAATTC
TATATATGGCTTAAAAAGCAAAAGCAACAAACCCTTCCCAAAAGCACCTTTGGTCAGGCTATTACTTACTGCCTGAACCAGTGGGATTGTTTAAACAGCT
TCCTGCTGGATGGCAGGTTGGAGATAGATAATAATCGTGCCGAACGTTCAATTAAACCCTTCGTAATAGGAAGGAAAAATTGGCTGTTTACAAATACCCC
CAGGGGTGCTAGAGGCAGTGCAATTATATACAGCGTAATTGAAACGGCCAAGGAGAATAACCTAAAGCCTTACAATTATATGTTTTATCTGTTTGAGCAG
TTGCCCAATGTTGATACGGGTGATCAAGCTGCCATTGACCGCTTGTTGCCCTGGTCTGATACACTGCCCGAGGAGTGCGGAATGCCCCAGGAGAATATTA
CCTCAAGTAGTTCCTGAGGGCTAGGTGTGCGCTATTTGACGGTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
312 bp	103 aa	86	397	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MVNNDLRSLWEQRLADYETSGKSIATWCREHSIRNNQFYYWRKKLRMDQVENNQPVKWLPLEVEQANLAPGSIGVHVGQATVEIKPGFDPHLLRQIVKVL
QTI

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
354 bp	117 aa	394	747	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MISEISNRLVYLACGSTDLRKSIDGLAVLVKESFHLDPFSPCLFVFCNRKRDKLKILQWEHNGFWLHYRRLERGKFDWPTAHTDVVSISYREFRWLLDGL
SLKQNQAHKAVKQRVIL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1593 bp	530 aa	825	2417	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNMQDNSAQIIEQLNDKILMQEQQIAELNAKVKWYEEQYRLSRQKQFGTSSEKTTPEQINLFNEAEDIVDPEIKEPDIETITYERKKKQPGQKADKLKDL
PVEVIEYRLLEHEQVCPCCQGSLHEMSTQIRQEIKVIPAQVKVVQHVQYIYSCRQCEKENITTPIIKAQMPNPILPGSLASPSILAYIMDQKYTNSMPLY
RQEQQLSRLGIELSRQTMANWVLNVADPWLKIIYDRLHVELLDRDILHADETTLQVLKEPGRSAETKSYMWLYRTGRDGPPIVLYEYQTTRASKHPDRFL
SGFKGYLQTDGYSAYGKLTGITLVGCWAHARRKFTEALKALPAAQKDKPVAASVGLEYCNRLFAIERQLKDVSDKERYDKRLEKSKPLLDEFYIWLKKQK
QQTLPKSTFGQAITYCLNQWDCLNSFLLDGRLEIDNNRAERSIKPFVIGRKNWLFTNTPRGARGSAIIYSVIETAKENNLKPYNYMFYLFEQLPNVDTGD
QAAIDRLLPWSDTLPEECGMPQENITSSSS

Blast result :

Comments

ISSwo2 is 47%(orfA), 74%(orfB), and 62%(orfC) aa similar to ISDeal1 .

References

1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Sims,D., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Boone,D.R., Brockman,F., Culley,D., Ferry,J., Gunsalus,R., McInerney,M.J., Morrison,M., Plugge,C., Rohlin,L., Scholten,J., Sieber,J., Stams,A.J.M. and Richardson,P. (2006) Direct submission GenBank.