ISArma1
- Family IS607
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ABYK01000012 | ND | Arthrospira maxima | Arthrospira maxima CS-328 |
DNA section
IS Length : 1766 bp
Ends
IR Length : 0
IRL : GAGCATGATTTAATCTCAAATTAGGTTAATATTTTAGGGCAATATGGTAT
IRR : CTGATACATTTACGCGACATTTATCCGGCTATTGCCTGACAATGCGACGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCAATACCAAAACGCTTGGA | TGAACCACTAATAGGGTATC | 0 |
DNA sequence
GAGCATGATTTAATCTCAAATTAGGTTAATATTTTAGGGCAATATGGTATAAGAAGCGAGAAGAGTTGAGGATAAACCTATTGCCAGATATGTCAAACCC
AAGGAAGCGGCTCAAATCCTTGGAGTCCATGAAAGAACACTCCGCAGATGGGACGAAAATGGCTCAATCGAGACCATCAGAACCCCCGCTGGGCAACGAC
GATACAACGTTGAGTCATATACTGCCAAATCAGGCAGTGACAAACGCAAAGTCGTTATCTATGCCAGAGTTAGTAGCCGCGCCCAGCAGTCAGACCTCAA
CCGACAGGTGGCCGCACTGTCCAACCTCTACCCCGAAGCAGAAGTCGTTTCAGAAATCGGAGGCGGGCTCAACTTCAAGGGAAAGAAAATGCTGGCCTTA
CTGGGACATCATTTGTCAGGAGATGTCCGCATGGTTGTCGTTGCCCACAAAGACCGATTGGCAATATGGGGATTTGACTTGTTTCGATGGCTCTGTGAGC
AAAACAGGTGCTCACTCATGGTTCTCAACCAGACAAGTCTCAGTCCAGAACCAGAAATGGTTGAGGACATCCTCACTATCCTCCACTGCTTCAGTTCCCG
ATTATACGGACGGAGTAAATACAAAACTCAGGTCAAAGAAGATCCGGATTTACCCCAGCCCAGAGCTAAATCAAGTCTGGCGTAAATGGCTGGCTGCTTG
TCGGTATTGCTACAACCAAGCAATTGCATTATCCCGGAGTGGTAAACGACTAAGCAAGTTAAAGTTACGCAATAAAGTGATGCCGAGTGACTTACCCGAA
TGGGTCAAAGAAACACCCTGCCACATTCGGCAAAATGCCATCTTTGATGCCTATCAGGCTTTGACCGCCAGTCCTGATGCCAGGTTTAGAAGTTGTCGTG
ACAGCTCTCAAGGGATTAAGTTCAATAATACTAATTTCTCTTCAGGGAGTTGGTATCCAAGACTAACGAAAGGATTAACTTTCATGGTTTCCGAACCCAT
CCCTAAAACTTGCGGGCAAGGGACTCAGTTGGTGTTTACCAAAGGTCGATGGTTGGCGATTTTCCCTGAACCAGTTGCCGTTACCCCAACTGACGCTACT
GGCGTGATTGCATTAGACCCGGGCGTCCGAACTTTTATGACCGGGTTTGATGGTTCACGGTTTCTGGAATTGGGCTCCGGGGATATTGGACGCATTACTA
GGCTATGTCAACATTTGGATGATTTGATGAGCCGAATCGCCAAGGAACCCTGTCGTTCAAGAAGGCGACGGATGAGGCAAGCGGCTCAACGAATGAGAAC
CAAAATCCGCAATCTAGTTGATGAAGCCCACAAACAAATTGCTCACTACTTGACTCACAACTACAGCATAATTTTTCTGCCGACCTTCGAGACTTCCGAT
ATGGTTGCCAAGGTGAAGCGTCTAATTAGGTCTAAGACTGCCCGCGCCATGCTGACATGGGCGCATTATCGATTCAAACTAACCCTGAGACATCAAGCCG
AGATAACTGGAACCACAGTTGTAGATGTGACGGAAGAATACACCAGCAAAACCTGTACTCACTGTGGTCAGATGCATTCCCAGCTAGGTGGCTCAAAAGT
GTTCCGATGTCCTGAGTGTGGGTTCACTCTACCCAGGGACTGGAACGGTGCTTTTGGAATCTTTCTAAAAGCTTTGCGGGATACCGCCTCTGTTACCTTA
ACGGGTAATAGTGCTATCGTCGCATTGTCAGGCAATAGCCGGATAAATGTCGCGTAAATGTATCAG
AAGGAAGCGGCTCAAATCCTTGGAGTCCATGAAAGAACACTCCGCAGATGGGACGAAAATGGCTCAATCGAGACCATCAGAACCCCCGCTGGGCAACGAC
GATACAACGTTGAGTCATATACTGCCAAATCAGGCAGTGACAAACGCAAAGTCGTTATCTATGCCAGAGTTAGTAGCCGCGCCCAGCAGTCAGACCTCAA
CCGACAGGTGGCCGCACTGTCCAACCTCTACCCCGAAGCAGAAGTCGTTTCAGAAATCGGAGGCGGGCTCAACTTCAAGGGAAAGAAAATGCTGGCCTTA
CTGGGACATCATTTGTCAGGAGATGTCCGCATGGTTGTCGTTGCCCACAAAGACCGATTGGCAATATGGGGATTTGACTTGTTTCGATGGCTCTGTGAGC
AAAACAGGTGCTCACTCATGGTTCTCAACCAGACAAGTCTCAGTCCAGAACCAGAAATGGTTGAGGACATCCTCACTATCCTCCACTGCTTCAGTTCCCG
ATTATACGGACGGAGTAAATACAAAACTCAGGTCAAAGAAGATCCGGATTTACCCCAGCCCAGAGCTAAATCAAGTCTGGCGTAAATGGCTGGCTGCTTG
TCGGTATTGCTACAACCAAGCAATTGCATTATCCCGGAGTGGTAAACGACTAAGCAAGTTAAAGTTACGCAATAAAGTGATGCCGAGTGACTTACCCGAA
TGGGTCAAAGAAACACCCTGCCACATTCGGCAAAATGCCATCTTTGATGCCTATCAGGCTTTGACCGCCAGTCCTGATGCCAGGTTTAGAAGTTGTCGTG
ACAGCTCTCAAGGGATTAAGTTCAATAATACTAATTTCTCTTCAGGGAGTTGGTATCCAAGACTAACGAAAGGATTAACTTTCATGGTTTCCGAACCCAT
CCCTAAAACTTGCGGGCAAGGGACTCAGTTGGTGTTTACCAAAGGTCGATGGTTGGCGATTTTCCCTGAACCAGTTGCCGTTACCCCAACTGACGCTACT
GGCGTGATTGCATTAGACCCGGGCGTCCGAACTTTTATGACCGGGTTTGATGGTTCACGGTTTCTGGAATTGGGCTCCGGGGATATTGGACGCATTACTA
GGCTATGTCAACATTTGGATGATTTGATGAGCCGAATCGCCAAGGAACCCTGTCGTTCAAGAAGGCGACGGATGAGGCAAGCGGCTCAACGAATGAGAAC
CAAAATCCGCAATCTAGTTGATGAAGCCCACAAACAAATTGCTCACTACTTGACTCACAACTACAGCATAATTTTTCTGCCGACCTTCGAGACTTCCGAT
ATGGTTGCCAAGGTGAAGCGTCTAATTAGGTCTAAGACTGCCCGCGCCATGCTGACATGGGCGCATTATCGATTCAAACTAACCCTGAGACATCAAGCCG
AGATAACTGGAACCACAGTTGTAGATGTGACGGAAGAATACACCAGCAAAACCTGTACTCACTGTGGTCAGATGCATTCCCAGCTAGGTGGCTCAAAAGT
GTTCCGATGTCCTGAGTGTGGGTTCACTCTACCCAGGGACTGGAACGGTGCTTTTGGAATCTTTCTAAAAGCTTTGCGGGATACCGCCTCTGTTACCTTA
ACGGGTAATAGTGCTATCGTCGCATTGTCAGGCAATAGCCGGATAAATGTCGCGTAAATGTATCAG
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 621 bp | 206 aa | 65 | 685 | + | No |
Chemistry : Serine
ORF sequence :
VEDKPIARYVKPKEAAQILGVHERTLRRWDENGSIETIRTPAGQRRYNVESYTAKSGSDKRKVVIYARVSSRAQQSDLNRQVAALSNLYPEAEVVSEIGG
GLNFKGKKMLALLGHHLSGDVRMVVVAHKDRLAIWGFDLFRWLCEQNRCSLMVLNQTSLSPEPEMVEDILTILHCFSSRLYGRSKYKTQVKEDPDLPQPR
AKSSLA
GLNFKGKKMLALLGHHLSGDVRMVVVAHKDRLAIWGFDLFRWLCEQNRCSLMVLNQTSLSPEPEMVEDILTILHCFSSRLYGRSKYKTQVKEDPDLPQPR
AKSSLA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1263 bp | 420 aa | 495 | 1757 | + | No |
AG : TnpB
ORF sequence :
VSKTGAHSWFSTRQVSVQNQKWLRTSSLSSTASVPDYTDGVNTKLRSKKIRIYPSPELNQVWRKWLAACRYCYNQAIALSRSGKRLSKLKLRNKVMPSDL
PEWVKETPCHIRQNAIFDAYQALTASPDARFRSCRDSSQGIKFNNTNFSSGSWYPRLTKGLTFMVSEPIPKTCGQGTQLVFTKGRWLAIFPEPVAVTPTD
ATGVIALDPGVRTFMTGFDGSRFLELGSGDIGRITRLCQHLDDLMSRIAKEPCRSRRRRMRQAAQRMRTKIRNLVDEAHKQIAHYLTHNYSIIFLPTFET
SDMVAKVKRLIRSKTARAMLTWAHYRFKLTLRHQAEITGTTVVDVTEEYTSKTCTHCGQMHSQLGGSKVFRCPECGFTLPRDWNGAFGIFLKALRDTASV
TLTGNSAIVALSGNSRINVA
PEWVKETPCHIRQNAIFDAYQALTASPDARFRSCRDSSQGIKFNNTNFSSGSWYPRLTKGLTFMVSEPIPKTCGQGTQLVFTKGRWLAIFPEPVAVTPTD
ATGVIALDPGVRTFMTGFDGSRFLELGSGDIGRITRLCQHLDDLMSRIAKEPCRSRRRRMRQAAQRMRTKIRNLVDEAHKQIAHYLTHNYSIIFLPTFET
SDMVAKVKRLIRSKTARAMLTWAHYRFKLTLRHQAEITGTTVVDVTEEYTSKTCTHCGQMHSQLGGSKVFRCPECGFTLPRDWNGAFGIFLKALRDTASV
TLTGNSAIVALSGNSRINVA
Blast result :
Comments
ISArma1 is 70% (ORFA : the transposase) and 73% (ORFB) aa similar to ISSoc2.
References
1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Bryant,D.A. (2008) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L. and Bryant,D.A. (2008) Direct submission GenBank.