ISfinder Mirror Site

ISMno13

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_011894	ND	Methylobacterium nodulans	Methylobacterium nodulans ORS 2060 Methylobacterium nodulans ORS 2060 plasmid pMNOD01

DNA section

IS Length : 2459 bp

Ends

IR Length : 25/42

IRL : GTAAGCGTCGTTTTGGAGGCACCTTTCGGGAGCTCGGCTGAGCATGGGAT
IRR : GTAAGCGTCTTCCTCAGGCCACATCCCTGAGCGTGGGCGTGACCGGGTAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCGGGATCGC	CGTGGGAG	AACGGGTATT	8
AGGCGCTTTC		ATCCGAACGG	0
TGGCCCTCAC		ATCCGAACGG	0

DNA sequence

GTAAGCGTCGTTTTGGAGGCACCTTTCGGGAGCTCGGCTGAGCATGGGATACGGAGCGGGTCAGGAGCTCCGTCCTATGTCGATGTCAGAGCGTATGGCT
ATGTCGGAGCCGGTCCGGCGCCTGGAGCTGTTCACCGGGGCCGGCCGGCGGCGGACGTGGTCGGAGGATGAGAAGGCGGCCATTGTCGCCGAGAGCGAGG
CGCCGAACACCTCGATCAGCGCGGTAGCGCGCCGGCATGGCCTGAGCGCCTCGCAGCTGTTCACGTGGCGGCGCTTGGCGCGCCAAGCGGCGATGGATGA
TCAGCAGGCCGAGCTGCGGTTCGTGCCGGCCGTGCTGATCTCGGAACCGCAGGAGCCGGCGTGTGCAGAGACGAGCCCCAAGCCGCGTTGGCGCCACGGC
TGTCACGGCATTGAGGTCGAAGTAGCTGGCGCCACGGTCCGCATCGGCGGGGAGGCGAGCGAGGCTCAGATTGCGGCGGTGATCCGGGCGCTGAAGGTCT
CGGCATGATCGGGCCGGCTGGGACAGTGCGCGTGATGCTGGCGACGCGGCCGGTGGACTTCCGCAAAGGTATCGACGGGCTGGCCGCGCTGGTGCGCGAG
GCGATGGGCGCGGATCCGTTCTCCGGCACGGTCTATGTGTTCCGCTCGAAGCGGGCAGATCGGACCAAGCTTTTGTTCTGGGATGGAAGCGGGGTGGTTC
TCGCGGCCAAGCGTCTGGAGGACGGTCAATTCTGCTGGCCGAAGGCTCAGGACGGCGTCGTGCGCCTCACGGCTGCGCAGCTCTCGGCCCTGCTCGAAGG
GCTGGACTGGAAGCGTGTCCACGAGGCTCGTCAGGTGAGCGCGCCAGCGGTGGCAGGCTGAGCAAAAAGAACGGCGCGAGGGGACGTCGTACTGGTAAGT
GGCTGAGTGATCTGATCTAATCCGCTTTGTGGCTGCGCCTGCTTCACCCTCGCCCGACGATCCCGAGACGCTCAAGGCGCTGCTCGCCGAGGAGCGGGCC
GAGAACGAGCGGCTGCGCCAGATCATCAAGGCGATGCAGCGTCATCGCTTCGGCCGTCGCGCCGAGAGCCTGCCCGAGGACCAGCTCCTGCTCGGGCTGG
AGGAGGCCGAGCAGGTCGAAGCCGAGGGTCTTGCGGCCGAGGAAACCGCGCCGGCGGTGAGGGCCGAGCGTACAGCGCGCCGGCGCAACAATCGCGGGGC
CCTGCCCGCCCATCTGCCGCGCGTCGAGCGCGTGGTCGACATCGAGAGCCCCGTCTGCCCCTGCTGCTCGGGCAGCCTGCACCGGATCGGCGAGGACGTG
TCCGAGCGCCTCGACATCGTACCGGCCCAAGTCCGGGTCATTGTGATCCGGCGCCCGCGTTACGCGTGCCGTGCCTGCGAGGCTATTGTGCTGCAGGCGT
CCGCGCCGGCGCGGCTGATCGAGGGCGGGTTGCCCACCGACGACCTCGTCGCGCACGTGCTCGTCGCCAAGTACGCCGACCACCTGCCACTCTATCGTCA
GGCACAGATCTTCGCGCGTCAGGGCATAGCTCTCGACCGCTCGACGCTCGCGGATTGGGTCGGCCGTGCTGCGTTCCTGCTGCGGCCGGTCCACGCGCGG
CTGCTGGGGAGGCTGAAGGCTTCGGGCAAGCTGTTTGCCGACGAGACCAGCGCGCCGGTGCTCGATCCCGGACGCGGCCGCACCAAGACAGGCCAGCTCT
GGGCGTATGCCCGCGACGACCGGCCCTGGGGCGGCACCGATCCGCCGGGCGTTGCCTACGTCTACGCACCCGACCGCACGGCCAAGCAGCCCATCGCGCA
TCTTGCCGGCTTCCGGGGCGTGCTGCAGGTCGATGGCTATGACGGCTACAAGGCGCTGGCCAGGCAAGGCGAGGTCCGGCTCGCCTACTGCTGGAGCCAT
GTACGGCGGGCGTTCTACGATCTTGATGGGCCGATCGCGGCCGAGGTGCTCACGCGCATCGCGGGCCTTTACCGGATCGAGGCCGAGATCCGCGGCCGTC
CTGCCGAGGAGCGCCGCACCGCACGTCAGGCGCGCAGCCGTCCCATCCTGGAGGCCCTAGAGCCCTGGCTGCGGGAGAAGCTCGCCCTCATCAGCCAGAA
GAGCAAGCTGGCCGAAGCGATCCGCTACGCGCTGAGCCGCTGGGCTGGCCTGAGCCTGTTCCTCGATGACGGGCGGGTCGAACTCGACACCAACGTGGTC
GAGCGCGCGATCCGTCCCCTGGCCCTGACGAGAAAGAATGCTCTCTTCGCCGGCTCGGATGGCGGGGCCGCGCACTGGGCGGTGATCGCCTCGCTGATCG
AGACCTGCAAGCTCACGGGCGTCGGACCGCAGGCCTATCTTGCCGACGTGATCACCCGCATCATCGAGGGCCATCCCAACAGCCGCATCGACGAGCTGCT
GCCCTGGGCCTACCCGGTCACGCCCACGCTCAGGGATGTGGCCTGAGGAAGACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
432 bp	143 aa	77	508	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSMSERMAMSEPVRRLELFTGAGRRRTWSEDEKAAIVAESEAPNTSISAVARRHGLSASQLFTWRRLARQAAMDDQQAELRFVPAVLISEPQEPACAETS
PKPRWRHGCHGIEVEVAGATVRIGGEASEAQIAAVIRALKVSA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
357 bp	118 aa	505	861	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIGPAGTVRVMLATRPVDFRKGIDGLAALVREAMGADPFSGTVYVFRSKRADRTKLLFWDGSGVVLAAKRLEDGQFCWPKAQDGVVRLTAAQLSALLEGL
DWKRVHEARQVSAPAVAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1518 bp	505 aa	929	2446	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VAAPASPSPDDPETLKALLAEERAENERLRQIIKAMQRHRFGRRAESLPEDQLLLGLEEAEQVEAEGLAAEETAPAVRAERTARRRNNRGALPAHLPRVE
RVVDIESPVCPCCSGSLHRIGEDVSERLDIVPAQVRVIVIRRPRYACRACEAIVLQASAPARLIEGGLPTDDLVAHVLVAKYADHLPLYRQAQIFARQGI
ALDRSTLADWVGRAAFLLRPVHARLLGRLKASGKLFADETSAPVLDPGRGRTKTGQLWAYARDDRPWGGTDPPGVAYVYAPDRTAKQPIAHLAGFRGVLQ
VDGYDGYKALARQGEVRLAYCWSHVRRAFYDLDGPIAAEVLTRIAGLYRIEAEIRGRPAEERRTARQARSRPILEALEPWLREKLALISQKSKLAEAIRY
ALSRWAGLSLFLDDGRVELDTNVVERAIRPLALTRKNALFAGSDGGAAHWAVIASLIETCKLTGVGPQAYLADVITRIIEGHPNSRIDELLPWAYPVTPT
LRDVA

Blast result :

Comments

ISMno13 is 64%(orfA), 88%(orfB) and 85%(orfC) aa similar to ISAzca2.

References

1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.