ISMch5
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011758 | ND | Methylobacterium chloromethanicum | Methylobacterium chloromethanicum CM4 plasmid pMCHL01 |
DNA section
IS Length : 1240 bp
Ends
IR Length : 24/27
IRL : TGGCCTGCTGCCGGTTTCACGGACACCCTGTTTAGGTGTGAAGGTGAAGC
IRR : TGGCCTGCTGCCGGTTTGTTGGACACCGTCCTAAGCTAATTGGGTTCGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGATCCGGC | CCTCGTTCTG | 0 |
DNA sequence
TGGCCTGCTGCCGGTTTCACGGACACCCTGTTTAGGTGTGAAGGTGAAGCCGGAGGTGCTCCATGCAGCGTCGCAAGTTCGGACGTGAGTTCAAGATCGA
GGCGGTTCGACTGATTCGCGAACGTGGTGTCAGTGTCGCGCAGGCCGCGCGCGATCTCGATGTTCACGAGACGATGCTGCACCGGTGGGTGAGGCAGGCG
GCGGCCGATCCGCGGCACGCCTTTCCCGGTCAGGGCCAGATGAAGCCCGAGCAGATCGAGATCGACCGGCTGCGCAAGGAGGTCGCTCGTCTAAAAGCGG
AGCGTGACATCCTAAAAAAGGCCGCCGCATACTTTGCGAGGGACGCGATATGAAGTTCGCGTTCATCGCAAAGCATCGTGCTGTCTGGCCAGTTGCCTGG
ATGTGCGCGGCGCTGGGTGTCTCGCGCTCCGGCTTCCACGCTTGGCTCACCCGGCAGCCTAGCCGACGCACACGGGACGACGAAGCTATCCTGAGCAAGG
CCCACGCGAGCTTCGTGGCCAGCGACCGCACCTACGGCGCGAGGCGCGTCTGGCGCGATGTGCTCGCCCAGGGCGTGTCATGCGGGCTGCACCGCATCGA
GCGGATCATGCGCGAGAATGCCTTGCGAGCGCGACCGCGCCGACGGGGCCTGCCAAAGGATGAAGGTGAGCGGGCCGCTGCCTCGCCCAATCTCCTGGAG
CGGCGGTTCGCGGCCGACGCTCCGAACCGGAAGTGGATCGCCGACTTCACGTATATCTGGACCGCCGAGGGCTGGCTCTACGTCGCCGCCGTCATCGATC
TGTTCTCGCGCCGTGTCGTGGGCTGGTCGATGCAGGCCAGTATGACGGCTCAACTCGTGACAGATGCGCTCGTCATGGCGATCTGGCGCAGAGGCAAGCC
GGATGCCTTGCTGCATCACTCGGACCAGGGCTCGCAATACACGAGCGAAGCCTTCCAGCGTCAAATGACCGAGCACGGCGTGACCTGCTCGATGAGCCGT
TCGGGCAACGTCTGGGACAACGCGGCAATGGAGAGCTTCTTCTCCTCGCTGAAGACCGAGCGCACCGCCCGACGGACCTACCGGACGCGGGATGAAGCCC
GTGCAGACGTGTTCGATTACATCGAGCGCTTCTACAACCTGGCGCGGCGGCACTCGACCTTGGGCTACCTCAGCCCCGTCGAGTTCGAAAACCGAACCCA
ATTAGCTTAGGACGGTGTCCAACAAACCGGCAGCAGGCCA
GGCGGTTCGACTGATTCGCGAACGTGGTGTCAGTGTCGCGCAGGCCGCGCGCGATCTCGATGTTCACGAGACGATGCTGCACCGGTGGGTGAGGCAGGCG
GCGGCCGATCCGCGGCACGCCTTTCCCGGTCAGGGCCAGATGAAGCCCGAGCAGATCGAGATCGACCGGCTGCGCAAGGAGGTCGCTCGTCTAAAAGCGG
AGCGTGACATCCTAAAAAAGGCCGCCGCATACTTTGCGAGGGACGCGATATGAAGTTCGCGTTCATCGCAAAGCATCGTGCTGTCTGGCCAGTTGCCTGG
ATGTGCGCGGCGCTGGGTGTCTCGCGCTCCGGCTTCCACGCTTGGCTCACCCGGCAGCCTAGCCGACGCACACGGGACGACGAAGCTATCCTGAGCAAGG
CCCACGCGAGCTTCGTGGCCAGCGACCGCACCTACGGCGCGAGGCGCGTCTGGCGCGATGTGCTCGCCCAGGGCGTGTCATGCGGGCTGCACCGCATCGA
GCGGATCATGCGCGAGAATGCCTTGCGAGCGCGACCGCGCCGACGGGGCCTGCCAAAGGATGAAGGTGAGCGGGCCGCTGCCTCGCCCAATCTCCTGGAG
CGGCGGTTCGCGGCCGACGCTCCGAACCGGAAGTGGATCGCCGACTTCACGTATATCTGGACCGCCGAGGGCTGGCTCTACGTCGCCGCCGTCATCGATC
TGTTCTCGCGCCGTGTCGTGGGCTGGTCGATGCAGGCCAGTATGACGGCTCAACTCGTGACAGATGCGCTCGTCATGGCGATCTGGCGCAGAGGCAAGCC
GGATGCCTTGCTGCATCACTCGGACCAGGGCTCGCAATACACGAGCGAAGCCTTCCAGCGTCAAATGACCGAGCACGGCGTGACCTGCTCGATGAGCCGT
TCGGGCAACGTCTGGGACAACGCGGCAATGGAGAGCTTCTTCTCCTCGCTGAAGACCGAGCGCACCGCCCGACGGACCTACCGGACGCGGGATGAAGCCC
GTGCAGACGTGTTCGATTACATCGAGCGCTTCTACAACCTGGCGCGGCGGCACTCGACCTTGGGCTACCTCAGCCCCGTCGAGTTCGAAAACCGAACCCA
ATTAGCTTAGGACGGTGTCCAACAAACCGGCAGCAGGCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 63 | 353 | + | No |
Description : First part of the transposase
ORF sequence :
MQRRKFGREFKIEAVRLIRERGVSVAQAARDLDVHETMLHRWVRQAAADPRHAFPGQGQMKPEQIEIDRLRKEVARLKAERDILKKAAAYFARDAI
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 903 bp | 300 aa | 308 | 1210 | + | No |
Description : Second part of the transposase
ORF sequence :
HPKKGRRILCEGRDMKFAFIAKHRAVWPVAWMCAALGVSRSGFHAWLTRQPSRRTRDDEAILSKAHASFVASDRTYGARRVWRDVLAQGVSCGLHRIERI
MRENALRARPRRRGLPKDEGERAAASPNLLERRFAADAPNRKWIADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMQASMTAQLVTDALVMAIWRRGKPDA
LLHHSDQGSQYTSEAFQRQMTEHGVTCSMSRSGNVWDNAAMESFFSSLKTERTARRTYRTRDEARADVFDYIERFYNLARRHSTLGYLSPVEFENRTQLA
MRENALRARPRRRGLPKDEGERAAASPNLLERRFAADAPNRKWIADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMQASMTAQLVTDALVMAIWRRGKPDA
LLHHSDQGSQYTSEAFQRQMTEHGVTCSMSRSGNVWDNAAMESFFSSLKTERTARRTYRTRDEARADVFDYIERFYNLARRHSTLGYLSPVEFENRTQLA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 63 | 1210 | + | Yes |
Chemistry : DDE
ORF sequence :
MQRRKFGREFKIEAVRLIRERGVSVAQAARDLDVHETMLHRWVRQAAADPRHAFPGQGQMKPEQIEIDRLRKEVARLKAERDILKKGRRILCEGRDMKFA
FIAKHRAVWPVAWMCAALGVSRSGFHAWLTRQPSRRTRDDEAILSKAHASFVASDRTYGARRVWRDVLAQGVSCGLHRIERIMRENALRARPRRRGLPKD
EGERAAASPNLLERRFAADAPNRKWIADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMQASMTAQLVTDALVMAIWRRGKPDALLHHSDQGSQYTSEAFQR
QMTEHGVTCSMSRSGNVWDNAAMESFFSSLKTERTARRTYRTRDEARADVFDYIERFYNLARRHSTLGYLSPVEFENRTQLA
FIAKHRAVWPVAWMCAALGVSRSGFHAWLTRQPSRRTRDDEAILSKAHASFVASDRTYGARRVWRDVLAQGVSCGLHRIERIMRENALRARPRRRGLPKD
EGERAAASPNLLERRFAADAPNRKWIADFTYIWTAEGWLYVAAVIDLFSRRVVGWSMQASMTAQLVTDALVMAIWRRGKPDALLHHSDQGSQYTSEAFQR
QMTEHGVTCSMSRSGNVWDNAAMESFFSSLKTERTARRTYRTRDEARADVFDYIERFYNLARRHSTLGYLSPVEFENRTQLA
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISMch5 is 89% aa similar to ISMlo4.
ISMch5 is 89% aa similar to ISMlo4.
References
1] Ming-Chun Lee and David Robinson, direct submission.