ISEc31
- Family IS3
- Group IS3
Isoform Synonym(s) ISEc67
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011751 | ND | Escherichia coli | Escherichia coli SMS-3-5 Escherichia coli UMN026 |
DNA section
IS Length : 1258 bp
Ends
IR Length : 23/33
IRL : TGTAGTGGCACAGTAAATTTGGCCACCTGATTAAAGGTGATATCCTCACC
IRR : TGTAGTGGTCAAGTAATACTGGCCACGGTTTTACAGTAAAAATGGTATCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCACAGGTT | TGT | AGGCATGATA | 3 |
| TTTTGCCGTT | CAC | CTTAAGTGTC | 3 |
| AATTACCTTC | ATA | ACGTGGATAA | 3 |
DNA sequence
TGTAGTGGCACAGTAAATTTGGCCACCTGATTAAAGGTGATATCCTCACCACAACACAAAACAGGTGACTTAATGAACAAGAAAACCAAACGTACTTTCA
CCCCTGAATTCAGGCTGGAATGTGCACAGCTAATTGTTGATAAGGGCTACTCATATCGACAAGCCAGTGAAGCGATGAATGTCGGTTCTACCACGCTTGA
GAGTTGGGTGCGCCAGCTCAGGCGAGAGCGTCAGGGGATTGCGCCCTCTGCCACACCTATTACTCCAGACCAGCAACGTATCCGCGAACTGGAAAAGCAG
GTTCGCCGCCTGGAGGAACAAAATACGATATTAAAAAAGGCTACCGCGCTCTTGATGTCCGACTCGCTGAACGGTTCACGATAGTTGCCAGACTGAGTGA
CAGCCACTCAGTTGTCAGCCTCTGTTCTGCTCTGGAAATACACCGCAGCAGTTACCGGTACTGGCGAAAACGACGCGATACGGTTAATCCGGCGCGAGTC
AGGTTGTGCAGCGAAATACGCCGGGCGTGGAACCAAAGTAGGGGCTCTGCGGGGGCGCGCACGCTGGCTGAAATGCTGACCCAAAACGGCGTCCCGATGA
GCCGTTACCGTGCCGGGCGTCTGATGAAATATCTGAACCTGAGCAGTTGTCAGCCCGGAAAACATCAGTACAAAAATGCTCGTCAGGAACATACCTGTCT
GCCGAATCTGCTTGAGCGCCAGTTCGCTGTGCCCGAGCCAGATAGGGTATGGTGCGGAGATATTACGTATATCTGGGCAGGAAATCGCTGGTGCTATCTG
GCGGTCGTCATGGATCTTTTTGCCCGCAGGGTTATCGGCTGGAGCCTGTCAGCGAATGCCGATACTGCCCTGATAAGCAGTGCCCTGCGGATGGCGTATG
AGGTGCGTGGTCAGCCGCGGGACGTCATGTTCCATAGCGACCAGGGAAGTCAATATACAGGACTGAAATATCAACAACTTCTCTGGCGTTACAGGATAAA
GCAAAGTGTCAGCCGACGGGGAAACTGCTGGGATAACAGTCCAATGGAACGCTTCTTCCGCAGTCTGAAAACAGAATGGGTGCCAACCGATGGCTACACA
GGCAAAGACGTGGCCCGGCAGCAAATCAGCAGTTATATTCTGAATTATTACAATAGCGTCAGGCCTCACCATTATAACGGAGGGCTGACGCCGGAAGAGT
CAGAGAACAGATACCATTTTTACTGTAAAACCGTGGCCAGTATTACTTGACCACTACA
CCCCTGAATTCAGGCTGGAATGTGCACAGCTAATTGTTGATAAGGGCTACTCATATCGACAAGCCAGTGAAGCGATGAATGTCGGTTCTACCACGCTTGA
GAGTTGGGTGCGCCAGCTCAGGCGAGAGCGTCAGGGGATTGCGCCCTCTGCCACACCTATTACTCCAGACCAGCAACGTATCCGCGAACTGGAAAAGCAG
GTTCGCCGCCTGGAGGAACAAAATACGATATTAAAAAAGGCTACCGCGCTCTTGATGTCCGACTCGCTGAACGGTTCACGATAGTTGCCAGACTGAGTGA
CAGCCACTCAGTTGTCAGCCTCTGTTCTGCTCTGGAAATACACCGCAGCAGTTACCGGTACTGGCGAAAACGACGCGATACGGTTAATCCGGCGCGAGTC
AGGTTGTGCAGCGAAATACGCCGGGCGTGGAACCAAAGTAGGGGCTCTGCGGGGGCGCGCACGCTGGCTGAAATGCTGACCCAAAACGGCGTCCCGATGA
GCCGTTACCGTGCCGGGCGTCTGATGAAATATCTGAACCTGAGCAGTTGTCAGCCCGGAAAACATCAGTACAAAAATGCTCGTCAGGAACATACCTGTCT
GCCGAATCTGCTTGAGCGCCAGTTCGCTGTGCCCGAGCCAGATAGGGTATGGTGCGGAGATATTACGTATATCTGGGCAGGAAATCGCTGGTGCTATCTG
GCGGTCGTCATGGATCTTTTTGCCCGCAGGGTTATCGGCTGGAGCCTGTCAGCGAATGCCGATACTGCCCTGATAAGCAGTGCCCTGCGGATGGCGTATG
AGGTGCGTGGTCAGCCGCGGGACGTCATGTTCCATAGCGACCAGGGAAGTCAATATACAGGACTGAAATATCAACAACTTCTCTGGCGTTACAGGATAAA
GCAAAGTGTCAGCCGACGGGGAAACTGCTGGGATAACAGTCCAATGGAACGCTTCTTCCGCAGTCTGAAAACAGAATGGGTGCCAACCGATGGCTACACA
GGCAAAGACGTGGCCCGGCAGCAAATCAGCAGTTATATTCTGAATTATTACAATAGCGTCAGGCCTCACCATTATAACGGAGGGCTGACGCCGGAAGAGT
CAGAGAACAGATACCATTTTTACTGTAAAACCGTGGCCAGTATTACTTGACCACTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 37 | 384 | + | No |
Description : First part of the transposase
ORF sequence :
VISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEQNTILK
KATALLMSDSLNGSR
KATALLMSDSLNGSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1050 bp | 349 aa | 201 | 1250 | + | No |
Description : Second part of the transposase
ORF sequence :
ELGAPAQARASGDCALCHTYYSRPATYPRTGKAGSPPGGTKYDIKKGYRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARV
RLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYL
AVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYT
GKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVASIT
RLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYL
AVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYT
GKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVASIT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1214 bp | 404 aa | 37 | 1250 | + | Yes |
Chemistry : DDE
ORF sequence :
VISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEQNTILK
KGYRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYL
NLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFH
SDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTV
ASIT
KGYRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYL
NLSSCQPGKHQYKNARQEHTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAYEVRGQPRDVMFH
SDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDNSPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTV
ASIT
Blast result :
Comments
ISEc31 is 77% aa similar to ISShfr6.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Genoscope -,C.E.A. (2008) Direct submission to GenBank
2] Genoscope -,C.E.A. (2008) Direct submission to GenBank