ISfinder Mirror Site

ISPaen1

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_GG695980	ND	Paenibacillus sp.	Paenibacillus sp. oral taxon 786 D14

DNA section

IS Length : 1851 bp

Ends

IR Length : 20/22

IRL : ATTGAACATGAAATCGGAATGTAGCCTAGCCAAAATCTGAGAGATTCACG
IRR : CTTCAACATGAAATCGGAATGTGGATAACTCAGGCCTATGGATAACCCGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATAACGAGA	TA	AATCAATGAT	2

DNA sequence

ATTGAACATGAAATCGGAATGTAGCCTAGCCAAAATCTGAGAGATTCACGTTGTGACAAGGCATTTTCGTCATAAAAAGTTGCCAATTTTTTTGCAAAAT
AAACAGGACACTGCAAGCCTCTTGTCAAAGGTTAAACCACCACGAATAACCAACGAGAGGAAGTGTCCCTAGCAACATGGTACCACAAGCAACGAACCAT
GCGCCAGAACGAAAATCACGCCGCGAACGCCGAATTGAAATTCTTGCGGAGCGTCGCCGAATCAAGCAGATCATGAAGAAGTTGAGACCGGTCACATTCG
ACAACAACACCGTCACGGAAGCCGGGAACTTCCAGTTCCTTGCCAGGTTCAAGGAACTCATCGGTCTGGACACCCTTATCGAGAGTCACGTTCATCTGGA
ACAAACAGCCAACTGTGTCTACACGGCCGAAAAGCTCGTCGATCACCTGCTGGACTGCGCGCTACTCGGCCACACACGTTTTTCACATATGAATGCCCTG
CAGTCGGACCCTGGTTACCAGATCGTTACCGGCATCGAGCGTTTTCCTGACGAAAGCACCTTTCGCAATTTCCTGGGCAAACTCACCTGGAGCCATCTGA
TTCAGCTTGTCGCGTTAAACCGGGATTTGCTGGCACATAAGGCGCGGACGGGCGAAAAGCGCCTGGTCTGGATCGACATCGACGACACGGTCATCACGCT
GTTCGGGGAGCAGGAAGGCGCGACAAACGGCTACAATCCGCGTTACCACGGACGTCCCTCGTACAAGATCCGGGTTGCTTTCATCGCCGGAAGCGGCGAG
CTGATCCACTTGCAACTGAATCCCGGCAACATGAACGGCATGAAGGATTTGCTCTCGTTCGTTAAAGAAGTCGAAGCGATGCTGCCGCCGGAATGGATCG
TCGAAGGCATCCGGGCCGACTGCGGTTTTGCCGACGAAGCTGTCATGGCGTACGCCGAAGAACGCGGCTGGCAGTACATCTTCAAGCTCCCGAAGAAAGC
GACCGTCAAGAAGGCCATCGCTTATCTGCAGCAGCATCCCGCCTTTTGGGAGACGCTGAACGAAAAGGAAGAGCACATTACGTTCGCAGACGAGGATGAT
CGTTGGGCTGCTGCCGACATCCCGATTCTCCTTACCAACTGGGAGAAAGCCAGGCGCTGTGTCATCTACCGGGAAGCCCACGAAACCGAGCGTACAACCG
ATGGCCAATTGACGATGGCTCTCACGACCTATTCCTTCCAAGCCATTGTCACCAATACCGATCTCAAGCCGCTCATGCTGCTTCGCTCGTACAACAAGCG
CGCCAACATCGAGAATCGCATCGATGAATTGAAAGAAGGTTATGCGGTGGAACAAAACAGCCAACATTCCATGCTCCGCAACCTGCTGTTCAGCTGGATC
AAGGCCATTGCCTACAACGTGATGGTCTGGTTCAAACAGACGCTGATGCCGGAAACCTTGCAGCGGTGCGAAGTCCAAACGATTCGCCGAGCGGTGATCC
GGGTACCCGGCAATGTGGTCGGCTCCGGTCGCTACCAGCATATCCGGCTTGCGCCGAATCCGACGCTCGCTGGAATCGTCGATGCGATGCAGCAAAAGCT
CAGAGCATTGGCCATAAGCTTCATTCCGACTCTACATGCGGCCTAACGGTTCGATTCATCCCTGGCCATAACGGCATTTCGACCTGTAAAAATCAGGTCT
TCTTTGGTTGTGCAAAAAAACGCAGGATCACATCAGAACGTAAGAAGACTTAAAAGCCCGGCTCCAAAAACGCATTGAAACAGGCATTCCGTAGTGCGAT
GGCGGGTTATCCATAGGCCTGAGTTATCCACATTCCGATTTCATGTTGAAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1470 bp	489 aa	177	1646	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVPQATNHAPERKSRRERRIEILAERRRIKQIMKKLRPVTFDNNTVTEAGNFQFLARFKELIGLDTLIESHVHLEQTANCVYTAEKLVDHLLDCALLGHT
RFSHMNALQSDPGYQIVTGIERFPDESTFRNFLGKLTWSHLIQLVALNRDLLAHKARTGEKRLVWIDIDDTVITLFGEQEGATNGYNPRYHGRPSYKIRV
AFIAGSGELIHLQLNPGNMNGMKDLLSFVKEVEAMLPPEWIVEGIRADCGFADEAVMAYAEERGWQYIFKLPKKATVKKAIAYLQQHPAFWETLNEKEEH
ITFADEDDRWAAADIPILLTNWEKARRCVIYREAHETERTTDGQLTMALTTYSFQAIVTNTDLKPLMLLRSYNKRANIENRIDELKEGYAVEQNSQHSML
RNLLFSWIKAIAYNVMVWFKQTLMPETLQRCEVQTIRRAVIRVPGNVVGSGRYQHIRLAPNPTLAGIVDAMQQKLRALAISFIPTLHAA

Blast result :

Comments

ISPaen1 is 61% aa similar to ISDre3.

References

1] ISfinder annotation (2009)
2] Ward,D., Young,S.K., Kodira,C.D., Zeng,Q., Koehrsen,M., Alvarado,L., Berlin,A., Borenstein,D., Chen,Z., Engels,R., Freedman,E., Gellesch,M., Goldberg,J., Griggs,A., Gujja,S., Heiman,D., Hepburn,T., Howarth,C., Jen,D., Larson,L., Lewis,B., Mehta,T., Park,D., Pearson,M., Roberts,A., Saif,S., Shea,T., Shenoy,N., Sisk,P., Stolte,C., Sykes,S., Walk,T., White,J., Yandava,C., Allen-Vercoe,E., Strauss,J., Sibley,C., White,A., Lander,E., Nusbaum,C., Galagan,J. and Birren,B. (2009) Direct submission GenBank.